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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study with analytical monitoring. The study was terminated since none of the analytical values were in the required range.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report Date:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Batch number: 7346-05167 Hauptlauf
- Purity: 98.2 area-% (report No.: 05L00169)

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified
Details on test solutions:
- Preparatio of test solution: The test substance (100 mg/L) was stirred for approx. 10 min at 20 ± 2°C. The stock solution was clear and colorless. The test substance concentrations were prepared by dilution of a stock solution of 100 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Age at study initiation: < 24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Nominal and measured concentrations:
- Nominal concentrations: 0 (control), 0.781, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L based on test substance mass without correction for purity.
Details on test conditions:
TEST SYSTEM
- Test vessel fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4

EFFECT PARAMETERS MEASURED: Mobility

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: Yes, the concentrations for the study were selected based on a range finding test where the 48-h EC50 was 10 - 100 mg/L.
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
12.5 - 25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

The analytically determined concentration of the test substance in the stock solution was 4.58 mg/L (4.6% of the nominal value) at start of exposure, the analytical values in the test concentrations at start of exposure were in a range of 0 - 5.7% of the nominal concentration.

Applicant's summary and conclusion