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Diss Factsheets
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EC number: 204-909-5 | CAS number: 128-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- sub-chronic toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Experimental data from study report
Data source
Referenceopen allclose all
- Reference Type:
- review article or handbook
- Title:
- Unnamed
- Year:
- 1 962
- Reference Type:
- other: secondary source
- Title:
- Water Quality Criteria for Colored Smokes: Solvent Green 3
- Author:
- Kowetha A. Davidson and Patricia S. Hovatter
- Year:
- 1 986
- Bibliographic source:
- U.S. Army Medical Research and Development Command, Fort Detrick Frederick, Maryland 21701-5012, Army Project Order No. 86PP6810, December 1987, page no 1-95.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Subchronic repeated dose oral toxicity study of test chemical in rats.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- 1,4-bis(p-tolylamino)anthraquinone
- EC Number:
- 204-909-5
- EC Name:
- 1,4-bis(p-tolylamino)anthraquinone
- Cas Number:
- 128-80-3
- Molecular formula:
- C28H22N2O2
- IUPAC Name:
- 1,4-bis[(4-methylphenyl)amino]-9,10-anthraquinone
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material: 1,4-bis(p-tolylamino)anthraquinone (Solvent Green 3)- Substance type: Organic- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of the test chemical: D and C green 6
- IUPAC name: 1,4-bis[(4-methylphenyl)amino]-9,10-anthraquinone
- Molecular formula: C28H22N2O2
- Molecular weight: 418.4938 g/mol
- Substance type: Organic
Test animals
- Species:
- rat
- Strain:
- not specified
- Details on species / strain selection:
- No data
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- No data
Administration / exposure
- Route of administration:
- oral: feed
- Details on route of administration:
- No data
- Vehicle:
- other: Diet
- Details on oral exposure:
- No data
- Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- No data
- Duration of treatment / exposure:
- 6 weeks
- Frequency of treatment:
- Daily
Doses / concentrationsopen allclose all
- Dose / conc.:
- 100 other: mg/kg bw
- Remarks:
- Concentration : 0.1%
- Dose / conc.:
- 230 other: mg/kg bw
- Remarks:
- Concentration: 0.23%
- Dose / conc.:
- 550 other: mg/kg bw
- Remarks:
- Concentration: 0.55%
- Dose / conc.:
- 1 290 other: mg/kg bw
- Remarks:
- Concentration: 1.29%
- Dose / conc.:
- 3 000 other: mg/kg bw
- Remarks:
- Concentration: 3.0%
- No. of animals per sex per dose:
- No data available
- Control animals:
- not specified
- Details on study design:
- No data
- Positive control:
- No data
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes, survival were observed .
DETAILED CLINICAL OBSERVATIONS: Yes
BODY WEIGHT: Yes
FOOD CONSUMPTION AND COMPOUND INTAKE (if feeding study): Yes
- Food consumption for each animal determined and mean daily diet consumption calculated as g food/kg body weight/day: Yes
FOOD EFFICIENCY: No data available
- Body weight gain in kg/food consumption in kg per unit time X 100 calculated as time-weighted averages from the consumption and body weight gain data: No data
WATER CONSUMPTION AND COMPOUND INTAKE (if drinking water study): No data
OPHTHALMOSCOPIC EXAMINATION: No data
HAEMATOLOGY: No data
CLINICAL CHEMISTRY: No data
URINALYSIS: No data
NEUROBEHAVIOURAL EXAMINATION: No data - Sacrifice and pathology:
- GROSS PATHOLOGY: No data
HISTOPATHOLOGY: Yes - Other examinations:
- No data
- Statistics:
- No data
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- no effects observed
- Food consumption and compound intake (if feeding study):
- no effects observed
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- effects observed, treatment-related
- Neuropathological findings:
- not specified
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Histopathological findings: neoplastic:
- no effects observed
- Other effects:
- not specified
- Details on results:
- Mortality:
No effect on survival of treated rats were observed.
Clinical sign:
No signs of toxicity were observed in treated rats.
Body weight:
Body weights were within normal range.
Food consumption:
Food consumption were within normal range.
Gross pathology:
When treated with 550 and 3000 mg/kg/day, small thyroid gland and gross degenerative changes in the liver were observed in the treated rats.
Histopathology:
When treated with 550 and 3000 mg/kg/day, increase in vacuolated cells around the hepatic central vein of liver were observed in treated rats.
Effect levels
- Dose descriptor:
- NOAEL
- Effect level:
- 230 other: mg/kg bw
- Based on:
- test mat.
- Sex:
- not specified
- Basis for effect level:
- other: No effect on survival, body weight, food consumption and gross pathology
- Remarks on result:
- other: No toxic effects were observed
Target system / organ toxicity
- Critical effects observed:
- not specified
- System:
- other: not specified
- Organ:
- not specified
Applicant's summary and conclusion
- Conclusions:
- The No observed adverse effect level (NOAEL) was considered to be 230 mg/kg bw/day when rats were treated with test chemical orally in feed for 6 weeks.
- Executive summary:
In a 6-week dose range-finding study in rats, test chemical was administered in the diet at concentrations of 0.1, 0.23, 0.55, 1.29, or 3,0 percent (100, 230, 550, 1290 or 3000 mg/kg bw). There were no deaths or gross signs of toxicity. Food consumption and body weights were within normal range. The thyroid gland was small in animals fed the 0.55 (550 mg/kg bw/day) and the 3.0 (3000 mg/kg bw/day) percent diets, but histopathological evaluation did not reveal abnormalities in the thyroid gland. Gross degenerative changes in the liver were observed and confirme the by histopathological examination, which showed an increase in vacuolated cells around the hepatic central vein. No other effects were observed. Therefore, the No observed adverse effect level (NOAEL) was considered to be 230 mg/kg bw/day when rats were treated with test chemical orally in feed for 6 weeks.
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