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EC number: 208-796-3
CAS number: 542-02-9
STATISTICAL RESULTS:-The highest dose (300 mg/kg for males and 200 mg/kg b.w. for females) was estimated by five
pre-experiments to the suitable since higher concentrations were lethal. After treatment with
the test item the number of NCEs was not substantially increased as compared to the mean value
of NCEs of the vehicle control thus indicating that this substance at the indicated concentrations
had no cytotoxic effectiveness in the bone marrow. There was no biologically and statistically relevant enhancement in the frequency of the detected
micronuclei after administration of the test item at any dose level or sampling time as compared
to vehicle controle. 40 mg/kg b.w. cyclophosphamide administered orally was used as positive
control which showed a substantial increase of induced micronucleus frequency.-micronucleus test results(A)Male animalsTest group Dose Sampling Sampling NCEs per--- mg/kg b.w. time (hr) micronuclei(%) 2000 PCEsvehicle 0 24 0.02 1566BG 75 24 0.08 1562BG 150 24 0.09 1814BG 300 24 0.02 1582CP 40 24 1.11 2033Vehicle 0 48 0.01 1694BG 75 48 0.02 1639BG 150 48 0.08 1795BG 300 48 0.07 1755Vehicle 0 72 0.03 1610BG 75 72 0.04 1417BG 150 72 0.03 1549BG 300 72 0.02 1645(B)Female animalsTest group Dose Sampling Sampling NCEs per--- mg/kg b.w. time (hr) micronuclei(%) 2000 PCEsvehicle 0 24 0.03 1652BG 50 24 0.03 1563BG 100 24 0.09 1463BG 200 24 0.04 1889CP 40 24 0.98 1755Vehicle 0 48 0.01 2113BG 50 48 0.04 1727BG 100 48 0.01 1628BG 200 48 0.02 1945Vehicle 0 72 0.02 1577BG 50 72 0.02 1408BG 100 72 0.04 1638BG 200 72 0.01 1492 BG = benzoguanamine (2,4-diamino-6-phenyl-1,3,5-triazine)CP = cyclo phosphamide
In a CD-1 mouse
bone marrow micronucleus assay, 5
male and female were treated oral
gavage with this substance at
doses of 0, 75, 150, 300 mg/kg bw in male mice and 0, 50, 100, 200
mg/kg/bw. Bone marrow cells were
harvested at 24, 48, 72Hr post-treatment.
The vehicle was corn oil.
were no signs of toxicity during
the study. The positive control
induced the appropriate response. There was not a
significant increase in the frequency of micronucleated polychromatic
erythrocytes in bone marrow after any treatment time.
This study satisfies the
requirement for Test Guideline OECD 474 forin vivocytogenetic
As there is no data available on "in vitro mammalian chromosome
aberration" or "in-vitro micronucleus" genetic toxicity studies
(required by Annex VIII of REACH regulation) with Acetoguanamine this
endpoint was read-acrossed from Benzoguanamine to Acetoguanamine. This
was done in order to avoid unnecessary animal testing and to fulfill
the claim of REACH regulation Annex VIII ("data from structurally
related substances (read-across approach) should be assessed first
before new tests are carried out). Justification for Read-Across see
above in "Rationale for Reliability".
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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