3,7-dimethyloct-6-enenitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP compliant, non guideline study, limitations in design and/or reporting but otherwise adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The methods described by Hagan served as a guide. (E.C. Hagan, "Acute Toxicity", Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics (The Association of Food and Drug Officials of the United States, 1975), pp. 17-25

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethyloct-6-enenitrile

EC Number:

257-288-8

EC Name:

3,7-dimethyloct-6-enenitrile

Cas Number:

51566-62-2

Molecular formula:

C10H17N

IUPAC Name:

3,7-dimethyloct-6-enenitrile

Details on test material:

- Name of test material (as cited in study report): Citronellal Nitril, #04526, J79-261 (no further data)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 6 to 8 weeks
- Weight at study initiation: 200-266g
- Fasting period before study: 18 hours
- Housing: Galvanized cages with indirect bedding
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: At least two days

ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Doses:

3150, 3960, 4460, 6300, 7940 mg/kg bw

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

The acute oral toxicity of the test material was evaluated in 6-8 week old Wistar strain albino rats weighing 200-266g. The rats were dosed singly in groups of six per dose level. Animals were observed for signs of toxicity and mortality at 1, 3, 6 and 24 hours following dosing. Observations were made at least once daily thereafter for a total of 14 days. All surviving animals were sacrificed at the end of day 14, weighed and gross necropsy was performed.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4 490 mg/kg bw

95% CL:

>= 3 740 - <= 5 390

Mortality:

- 3150 mg/kg bw: 1/3 males, 0/3 females died.
- 3960 mg/kg bw: 1/3 males; 1/3 females died.
- 4460 mg/kg bw: 3/3 males; 2/3 females died.
- 6300 mg/kg bw: 3/3 males; 2/3 females died.
- 7940 mg/kg bw: 2/3 males; 3/3 females died.

Clinical signs:

other: - 3150 mg/kg bw: Clinical signs were normal. One animal died btw. day 7-14. - 3960 mg/kg bw: One animal showed slight depression for 24 hours, but recovered within 2 days. One animal died within two days and one animal died within 5 days. - 4460 mg/kg bw:

Gross pathology:

- 3150 mg/kg bw: The one animal that died had fibrous tissue encasing heart and lungs. In the other animals no gross pathology changes were observed.
- 3960 mg/kg bw: One of the four surviving animals showed fibrous tissue encasing the heart and lungs. In one of two animals that died during the observation period the following observations were made: head partially cannibalized, pyloric mucosa severely reddened, stomach ruptured, all abdominal viscera adhered to body wall and covered with a thin layer of fibrous tissue.
- 4460 mg/kg bw: In one animal which died during the observation period, a extremely pale liver and ruptured intestines were observed. Two other animals which died during the observation period where partially cannibalized.
- 6300 mg/kg bw: In three animals moderate reddened pyloric mucosa was observed. In the animal who died within one hour after treatment, test article was found in the stomach.
- 7940 mg/kg bw: One animal was partially cannibalized. In the other animals no gross pathology changes were observed.