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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: According to the German national standard test procedures, basic data given.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Principles of method if other than guideline:
Oxygen consumption test according to ROBRA which is comparable to the German National Standard, DIN 38412 Part 27 (draft), 1992.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3,7,11,15-tetramethylhexadec-1-en-3-ol
EC Number:
208-008-8
EC Name:
3,7,11,15-tetramethylhexadec-1-en-3-ol
Cas Number:
505-32-8
Molecular formula:
C20H40O
IUPAC Name:
3,7,11,15-tetramethylhexadec-1-en-3-ol
Details on test material:
- Name of test material (as cited in study report): 3,7,11,15-Tetramethyl-1-hexadecen-3-ol (Isophytol)

Purity: no data
Water solubility: insoluble

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution of the test substance (nominal 10,000 mg/l) was prepared using 100 mg/l Tween 80 as an emulsifier.

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Nominal and measured concentrations:
Nominal test concentrations: control, 50, 100 and 1000 mg/l.

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
EC10
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC90
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion