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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: cytogenicity / chromosome aberration
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data have been obtained from secondary source.

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
Toxicological Profile for dinitrophenol
Author:
M. Olivia Harris, M.A; James J. Corcoran, Ph.D. (peer rewied by Dr. martin Alexander; Dr.Leon Koller; Dr.Norman Trieff)
Year:
1995
Bibliographic source:
U.S Department of Health and Human Services, Public Health Service Agency for Toxic Substances and Disease Registry
Reference Type:
review article or handbook
Title:
Effect of some phenolic compounds on chromosomes of bone marrow cells in mice
Author:
Mitra AB, Manna GK.
Year:
1971
Bibliographic source:
Indian J Med Res 59:1442-1447.

Materials and methods

Principles of method if other than guideline:
The method is based on a chromosome aberration assay in vivo. An intraperitoneal injection of three saturated solution concentrations was made on mice. After 24 hours bone marrow cells were analyzed. No further details were specified.
Type of assay:
chromosome aberration assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: saturated solution

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
intraperitoneal
Post exposure period:
Mice were sacrified 24 hours posttreatment for analysis of bone marrow cells.
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
0.25 ml
Basis:
other: saturated solution
Remarks:
Doses / Concentrations:
0.5 ml
Basis:
other: saturated solution
Remarks:
Doses / Concentrations:
1 ml
Basis:
other: saturated solution

Examinations

Tissues and cell types examined:
Bone marrow cells was observed for chromosomal aberration analysis.

Results and discussion

Test results
Sex:
not specified
Genotoxicity:
positive
Toxicity:
not specified

Any other information on results incl. tables

The authors concluded that 2,4-DNP was clastogenic under the assay conditions and attributed the effect to the compound’s electrophilic properties.

However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): positive
2,4-DNP was clastogenic under the assay conditions due to compound’s electrophilic properties. However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.
Executive summary:

Data have been obtained from secondary source that mentions Mitra AB, Manna GK. 1971. Effect of some phenolic compounds on chromosomes of bone marrow cells in mice. Indian J Med Res 59:1442-1447.

The method is based on a chromosome aberration assay in vivo. Mice were injected intraperitoneally with 0.25, 0.50, and 1 mL of a saturated solution of 2,4-DNP, then sacrificed 24 hours posttreatment for analysis of bone marrow cells for chromosomal aberrations . No further details were specified.

The authors concluded that 2,4-DNP was clastogenic under the assay conditions and attributed the effect to the compound’s electrophilic properties.

However there was no linear relationship between the frequency of chromosome aberrations and the dose of 2,4-dinitrophenol.