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EC number: 238-270-9
CAS number: 14324-55-1
Table: Effects of the test item on
Nitrate-Content and Nitrate Rate (Mean Values)
[mg/kg soil dw]
Interval day 0-28
mg/kg dry weight
mg/d/kg dry weight
(1) Dev., deviation from control
(2) sig.: significance according to
Student-t-test procedure, one sided, α = 0.05;
n.s.: not significant
The 28-d NOEC value was determined to be
1000 mg/kg soil dw, as the test item had no impact on nitrogen
transformation (nitrate content and nitrate formation rate) of soil
toxicity to soil microorganisms was determined in a study according to
OECD 216 and in compliance with GLP criteria (Ibacon GmbH, 2017). In
this study, soil (silty sand) was incubated for 28 days with test
substance concentrations of 0 (control), 5, 20, 80, 320 and 1000 mg/kg
soil dw. The test substance was mixed with quartz sand and lucerne meal
(0.5%) and subsequently mixed with the soil. The soil contained
approximately 2.6% clay, 35% silt and 62.4% sand. For the determination
of nitrate-N content, soil samples were taken within 6 hours after
application and at test end at day 28. Nitrate content and the nitrate
formation rate were subsequently compared to that of the controls. No
adverse effects of the test item on nitrate content in soil were
observed. The soil nitrate formation rates did not exceed the trigger
range of ± 25% set by OECD guideline 216 at the 0 - 28 day
determination. Differences to the control were -0.33%, 4.62%, 11.77%,
19.03% and 17.05% for the lowest test concentration (5 mg test item / kg
soil dry weight) up to the highest test concentration (1000 mg test
item/kg soil dry weight). The differences were statistically not
significant at day 28 for all test rates. Based on these results, the
28-d NOEC value (based on the nitrate formation rate) is determined to
be 1000 mg/kg soil dw.
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