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EC number: 817-766-4 | CAS number: 91891-42-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 Oct - 22 Dec 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene
- EC Number:
- 817-766-4
- Cas Number:
- 91891-42-8
- Molecular formula:
- C6F12O
- IUPAC Name:
- 1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: MA-3-HO-ANL 1600356
- Expiration date of the lot/batch: 31 Aug 2020
- Purity test date: 05 Feb 2018
- Purity: 93.1%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature container flushed with nitrogen
- Stability under test conditions: Yes
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All concentrations sampled
- Volume: 42.5 mL, a complete vial (see sampling method). Generally only one replicate at each concentration was measured.
- Sampling method: Extra sample vials without daphnia were made to be sacrificed for analytic monitoring (one for t=0h and one for t=24h) and incubated under similar conditions to the vials containing daphnia. The vials containing daphnia were sampled for t=48 h.
- Sample storage conditions before analysis: Samples were analyzed on the day of sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Method: The test substance is volatile. Therefore, 42.5mL air-tight vials were completely filled with medium and closed with a septum-sealed screw cap. MA-3 (2.5 µL) was then injected through the septum with gas-tight syringe to achieve a loading rate of 100 mg/L. Thereafter the septum was sealed using parafilm. Vials were rotated slowly for four days, and then centrifuged at 500g for 90 minutes to pull dissolved test substance into a single drop at the bottom of the vial. A dilution series was also made of additional 100% saturated samples.
- Controls: Blank only
- Evidence of undissolved material: Test solutions were clear and colorless. Undiluted (100% saturated) samples contained a visible small droplet of undissolved test substance at the bottom of the vials.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna (Crustacea, Cladocera) (Straus, 1820).
- Source: In-house laboratory culture with a known history, at least third generation, obtained by
acyclical parthenogenesis under specified breeding conditions.
- Age of parental stock (mean and range, SD): > 2 weeks
- Age at study initiation: < 24 hrs
- Feeding during test: No
-Culturing: Adult daphnids were cultured in M7 medium. Adult were not more than 4 weeks old prior to collection of the juveniles for testing.
During the period preceding the test, water temperatures were 18 - 22°C. Daphnids in the cultures were fed daily a suspension of freshwater algae. The adults were fed prior to test initiation, but neonates were not fed during the test. Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg CaCO3 in the medium
- Test temperature:
- 19 - 21 °C
- pH:
- 7.9 - 8.1
- Dissolved oxygen:
- 8.3 - 9.2 mg/L
- Nominal and measured concentrations:
- See table 1
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass VOA vials closed with faced septa, completely filled. Fill volume ca. 42.5 mL
- Aeration: no
- No. of organisms per vessel:five
- No. of vessels per concentration (replicates): four
- No. of vessels per control (replicates):four
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO medium prepared using reverse-osmosis purified water (GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
- Intervals of water quality measurement: At the start of the test (0-hour), and at the end (48-hours)
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light; 8 hours dark - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2CrO7)
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 73 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI, 53-99 µg/L
- Details on results:
- Microscopic examination on Replicate C (Table 2) of the 100% saturated solution group showed no undissolved material adhering to immobilized organisms.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Dose-response test: Yes
- ECx: 48-hr EC50 = 0.40 mg/L (95% CI, 0.33 mg/L to 0.48 mg/L)
- Other: The sensitivity of Daphnia magna originating from the batch used in this test, for potassium dichromate fell within the expected range of sensitivity observed during previous years (48-hr EC50 between 0.28 and 0.90 mg/L.) The reference substance test was conducted between the rangefinder and final test (42 days before final testing) - Reported statistics and error estimates:
- The 24 and 48h-EC50-value was calculated from the percentages of affected daphnids and the logarithms of the corresponding test substance concentrations using the Spearman-Karber method (non-linear, without trimming).
ToxRat Professional v 3.2.1 (ToxRat Solutions® GmbH, Germany) was used to perform the analyses.
Any other information on results incl. tables
Table 2., Immobilization of daphnids as a function of dilution (percent of saturated solution)
Time (hr) |
Replicate |
Control (0% saturated solution) |
4.6% saturated solution |
10% saturated solution |
22% saturated solution |
46% saturated solution |
100% saturated solution |
24 |
A |
0 |
5¹ |
0 |
0 |
0 |
4 |
B |
0 |
0 |
0 |
0 |
0 |
5 |
|
C |
0 |
0 |
0 |
1² |
0 |
4 |
|
D |
0 |
1¹ |
0 |
0 |
0 |
0 |
|
Total |
0 |
6¹ |
0 |
1 |
0 |
13 |
|
% effect |
0 |
30 |
0 |
5 |
0 |
65 |
|
48 |
A |
0 |
5¹ |
0 |
0 |
0 |
5 |
B |
0 |
0 |
0 |
0 |
0 |
5 |
|
C |
0 |
0 |
0 |
0 |
0 |
5 |
|
D |
0 |
1¹ |
0 |
0 |
0 |
0³ |
|
Total |
0 |
6¹ |
0 |
0 |
0 |
15 |
|
% effect |
0 |
30 |
0 |
0 |
0 |
75 |
1, Immobile daphnia were stuck to the septum of the vial. Value not used for determination of effects concentration
2, Organism recovered mobility by end of test
3, Additional sample analysis showed considerably lower concentration in this vial v. another measurement (Table 1).
Table 3, Average concentrations
Percentage of 100 mg/L loading solution |
Average concentration (µg/L) |
0 |
not detected |
4.6 |
7.4 |
10 |
19 |
22 |
31 |
46 |
14 |
100 |
169 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- <10% immobilization in controls, DO >3mg/L in all test vessels
- Conclusions:
- The EC50 of MA-3 to D. magna is 73 µg/L (95% CI, 53 - 99 µg/L) (OECD202).
- Executive summary:
Toxicity of MA-3 to D. magna was assessed in a test conducted according to OECD 202. The test substance was not soluble at the limit of 100 mg/L and is extremely volatile. Water soluble fractions at 100 mg/L were made in closed VOA vials with septum closures. Dilution series were made using gas-tight syringes into further VOA vials. Four replicates were prepared of negative controls and at each concentration. Vials at the full loading rate intentionally contained undissolved test material, which was consolidated into a droplet by centrifugation before addition of daphnids. Test substance concentrations were determined at test initiation, 24 hrs, and 48 hrs. Initial and 24-hour concentrations were taken in separate abiotic vials set up for that purpose, while 48-h concentrations were taken from a single replicate. No significant immobilization was observed in any of the dilutions, with complete immobilization occuring in three of the four replicates of undiluted WAF. No test substance was found attached to immobilized organisms by microscopic examination of the third replicate. No immobilization was observed in the fourth replicate, although test substance concentration was less than observed in a different replicate of undiluted medium. The EC50 based on arithmatic mean concentration was 73 µg/L (95% CI, 53 - 99 µg/L).
The test was conducted according to an internationally accepted guideline and was GLP compliant. Test substance concentrations were analytically confirmed. However, the test substance could not be maintained consistently in dilutions, and one replicate with undissolved test material showed inconsistent effects and measured concentration. This test is therefore considered reliable with restrictions. It is suitable for Risk Assessment, Classification & Labeling, and PBT Analysis.
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