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EC number: 944-710-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-05-30 to 2018-07-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: Reaction mass of disodium 4-[[4-acetylmethylamino)-2-sulfonatophenyl]amino]-1-amino-9,10-dihydro-9,10-dioxoanthracene-2-sulfonate and sodium sulphate and sodium chloride
Batch No.: CHA0 122490
Certificate of Analysis / Date: February 12, 2015
EC 944-710-7
Physical Appearance / Colour: Dark blue powder
Expiry Date: February 12, 2025
Storage Conditions at Test Facility: At 20°C ± 5°C, in the dark - Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Preliminary Test (Tier 1)
Test Duration: The incubation was terminated after 5 days.
Sampling of the Test Samples: pH 4, 7 and 9: 0, 3, 24, 120 h
At each sampling point, samples were taken in duplicate. - Buffers:
- Sterile aqueous solutions buffered at pH 4, 7 and 9.
The pH of each buffer solution was measured with a calibrated pH meter.
pH 4: 0.05 M phthalate buffer
20 mL NaOH (0.1 M) was added to 250 mL potassium dihydrogenphosphate (0.1 M). The solution was filled up to 500 mL with pure water.
pH 7: 0.05 M phosphate buffer
148 mL NaOH (0.1 M) was added to 250 mL potassium dihydrogenphosphate (0.1 M). The solution was filled up to 500 mL with pure water.
pH 9: 0.05 M boric acid buffer
106.5 mL NaOH (0.1 M) was added to 250 mL of a solution of boric acid (0.1 M) in potassium chloride (0.1 M). The solution was filled up to 500 mL with pure water. - Details on test conditions:
- 50 °C ± 0.1 °C
Anoxic Conditions: To avoid oxidation the buffer solutions were purged with inert gas (nitrogen) prior to sterilisation.
Sterile Conditions: Degased buffer solutions and the used glassware were sterilised using an autoclave (20 min at 121°C) prior to application. - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 20 mg/L
- Remarks:
- 99.5% recovery
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 20 mg/L
- Remarks:
- 99.3% recovery
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 20 mg/L
- Remarks:
- 99.3% recovery
- Number of replicates:
- Two samples of solutions of each pH value were taken at each sampling point.
- Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- Samples were incubated at three different pH-values and at one temperature. The test was carried out for 5 days. During incubation the concentration of the test item remained stable at each tested pH-value of 4, 7 and 9. Recoveries (mean) were in the range of 99.3-100.3% of the nominal applied concentration. Thus the test item can be stated as hydrolytically stable. The main test (Tier 2) was not performed.
- Transformation products:
- no
- % Recovery:
- 99.6
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- 100
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 5 d
- % Recovery:
- 100.3
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 5 d
- Key result
- Remarks on result:
- hydrolytically stable based on preliminary test
- Validity criteria fulfilled:
- yes
- Remarks:
- Immediately after application (0 h) recoveries (mean) of the samples were as follows: 99.3-99.5% of the nominal applied concentration.
- Conclusions:
- The present study investigated the hydrolytic behaviour of the test item in aqueous solutions buffered at pH 4, 7 and 9 and at one elevated temperature. Samples were incubated in the dark. The test was carried out for 5 days. At the end of incubation, 99.6-100.3% of the nominal applied concentration were found and thus the test item can be stated as hydrolytically stable (t0.5 > 1 year at 25°C).
- Executive summary:
Test Item: Acid Blue 182
Guidelines/Recommendations:This study was based on the procedures indicated by the following internationally accepted guidelines and recommendations:
OECD Guideline for Testing of Chemicals No. 111: "Hydrolysis as a function of pH", adopted April 13, 2004.
GLP: Yes (certified laboratory)
Purpose: The purpose of the study was to determine the rate of hydrolysis of Acid BLue 182 at different environmentally relevant pH-values and at different temperatures.
Test Setup:
Test Vessels: Glass flasks were used for the test.
Aqueous Solution: Sterile aqueous solutions buffered at pH 4, 7 and 9.
Test Conditions: In the dark at,Preliminary test (Tier 1): 50°C ± 0.1°C
Treatment Rate: Preliminary test (Tier 1): 20.1 and 20.3 mg/L (two individual replicates)
Results:
The present study investigated the hydrolytic behaviour of the test item in aqueous solutions buffered at pH 4, 7 and 9 and at one elevated temperature. Samples were incubated in the dark. The test was carried out for 5 days. At the end of incubation, 99.6-100.3% of the nominal applied concentration were found and thus the test item can be stated as hydrolytically stable (t0.5 > 1 year at 25°C).
This study is classified acceptable and satisfies the guideline requirements for hydrolysis studies.
Reference
Description of key information
OECD 111
The present study investigated the hydrolytic behaviour of the test item in aqueous solutions buffered at pH 4, 7 and 9 and at one elevated temperature. Samples were incubated in the dark. The test was carried out for 5 days. At the end of incubation, 99.6-100.3% of the nominal applied concentration were found and thus the test item can be stated as hydrolytically stable (t0.5 > 1 year at 25°C).
Key value for chemical safety assessment
Additional information
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