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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 Jun - 10 Jul 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Testing Methods for New Chemical Substances
- Version / remarks:
- (March 31, 2011, No.0331-7, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare; March 29, 2011, No.5, Manufacturing Industries Bureau, Ministry of Economy, Trade and Industry; No. 110331009, Environmental Policy Bureau, Ministry of the Environment, Japan.) IV. Algae growth inhibition test
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 1.0; 3.2; 10; 32; 100 mg/L
- Sampling method: At initiation of exposure: an aliquot of remaining unused test solution of each test group was used for measurement. At the termination of exposure: the same quantity of the sample taken from each replicate was mixed and analyzed per each test group. The test solution was centrifuged (2000 g, 10 min, 22 °C) and the aqueous layer without algae was analyzed for test substance concentration (n = 1).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 0.1183 g of the test substance was weighed with the electronic balance. A stock solution was prepared by mixing the test substance with the medium to 100 mL and then stirred for 48 h with a magnetic stirrer at 22 ± 2 °C. Each test solution was prepared by diluting an appropriate volume of the stock solution with the medium to a volume of 300 mL.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: ATCC 22662
- Source: American Type Culture Collection (date received: 01 Aug 1996)
- Age of inoculum (at test initiation): 3 d
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.3 - 23.7 ° C
- pH:
- 7.6 - 7.8 at initiation of exposure
7.8 - 7.9 at termination of exposure - Nominal and measured concentrations:
- nominal: control, 1.0, 3.2, 10, 32, 100 mg/L
mean measured concentration: control, 1.04, 3.34, 10.4, 33.5, 104 mg/L - Details on test conditions:
- TEST SYSTEM
- Type: closed with an air-permeable silicone stopper
- Material, size, headspace, fill volume: glass; 300 mL; headspace: 250 mL; fill volume: 50 mL
- Initial cell density: 0.5 x 1E+04 cells/mL
- Control end cell density: Mean = 557500 cells/mL, S.D. = 62659 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous fluorescent illumination: cool-white light
- Light intensity and quality: Wave length: 400 - 700 nm; Light intensity: 60 - 120 μE/m²/s ± 15% (at the surface of the cultures)
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: electronic particle counter at 24, 48 and 72 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: yes
- Test concentrations: 1.0, 3.2, 10, 32 and 100 mg/L of test substance
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 104 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.34 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control: yes
- Colour differences: test solution was slighty yellow at nominal concentrations of 32 and 100 mg/L - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- ErC50 (0 - 72 h): 1.14 mg/L
- Historical ErC50 (0 - 72 h): 1.1 mg/L ± 0.26 mg/L (Mean ± S.D.)
Any other information on results incl. tables
Table 1. Concentrations of test substance in the test solutions.
Nominal concentration of test substance (mg/L) |
Measured concentration of test substance (mg/L) |
||
0 h |
72 h |
Mean |
|
Medium control |
< 0.15#1 |
< 0.15#1 |
- |
1.0 |
1.03 [103] |
1.05 [105] |
1.04 [104] |
3.2 |
3.31 [103] |
3.37 [105] |
3.34 [104] |
10 |
10.3 [103] |
10.6 [106] |
10.4 [104] |
32 |
33.1 [103] |
33.9 [106] |
33.5 [105] |
100 |
103 [103] |
105 [105] |
104 [104] |
[ ] : percentage to the nominal concentration (%)
#1: less than quantification limit
Table 2. Percent growth inhibition (Iµ) for growth rate of P. subcapitata exposed to test substance.
|
vessel n° |
growth rate (µ/d) |
|
Nominal concentration of test substance (mg/L) |
0 - 72 h |
Percent inhibition#1 Iµ(%) |
|
Medium control |
1 |
1.5241 |
- |
2 |
1.5506 |
- |
|
3 |
1.5628 |
- |
|
4 |
1.6210 |
- |
|
5 |
1.5525 |
- |
|
6 |
1.6068 |
- |
|
Mean |
1.5696 |
- |
|
SD |
0.0369 |
- |
|
CV |
2.3 |
- |
|
1.0 [1.04] |
1 |
1.5502 |
1.2 |
2 |
1.5000 |
4.4 |
|
3 |
1.5180 |
3.3 |
|
Mean |
1.5227 |
3.0 |
|
SD |
0.0255 |
- |
|
3.2 [3.34] |
1 |
1.5177 |
3.3 |
2 |
1.5993 |
-1.9 |
|
3 |
1.5986 |
-1.8 |
|
Mean |
1.5719 |
-0.1 |
|
SD |
0.0469 |
- |
|
10 [10.4] |
1 |
1.4246 |
9.2 |
2 |
1.4579 |
7.1 |
|
3 |
1.4912 |
5.0 |
|
Mean |
1.4579 |
7. 1† |
|
SD |
0.0333 |
- |
|
32 [33.5] |
1 |
1.4401 |
8.3 |
2 |
1.5487 |
1.3 |
|
3 |
1.5057 |
4.1 |
|
Mean |
1.4981 |
4.6 |
|
SD |
0.0547 |
- |
|
100 [104] |
1 |
1.4509 |
7.6 |
2 |
1.4373 |
8.4 |
|
3 |
1.4487 |
7.7 |
|
Mean |
1.4456 |
7.9† |
|
SD |
0.0073 |
- |
[ ]: Mean measured concentration of test substance
†:Statistically different (Multiple comparison (Dunnett test, significance level=5%))
#1: Percent inhibition relative to the medium control
SD: Standard deviation
CV: Coefficient of variation (%)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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