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EC number: 942-639-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11 February 2016 - 11 February 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Name: EPICLON EXA-7250
Batch/Lot No.: B006
Description: Brown solid (in smaller amount the colour is yellow)
Purity: >99%
Molecular formula: C28H28O6 + (C19H18O4)n
Manufacture date: 02 October 2014
Expiry date: 02 March 2017
Storage condition: Controlled room temperature (15-25oC, below 70 RH%)
Safety precautions: Routine safety precautions (gloves, goggles, face mask, lab coat) for unknown materials were applied to assure personnel health and safety. - Analytical monitoring:
- not specified
- Test organisms (species):
- activated sludge, domestic
- Test type:
- not specified
- Water media type:
- other: Deionised water
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- Temperature controlled laboratory
20.5 – 21.1 °C - pH:
- Based on this ratio, calculated amounts of wet sludge were suspended in chlorine-free tap water to yield a concentration equivalent to 3 g per litre (on dry weight basis). The pH of the activated sludge inoculum was determined to be pH 7.21. The activated sludge was used directly after conditioning.
- Dissolved oxygen:
- For the measurement of the respiration rate a well-mixed sample of each treatment was poured into a BOD flask after about 3 hours incubation time, and was not further aerated. The oxygen concentration was measured for about fifteen minutes, with an O2 electrode and was recorded in every half minute. The oxygen consumption (in mg O2 L-1 minute-1) was determined from the most linear part of the respiration curve.
- Nominal and measured concentrations:
- 10, 100, and 1000 mg/L
- Details on test conditions:
- Aeration: With compressed air (about 2 L/min)
Recording: Test conditions were measured with suitable instruments and documented in the raw data. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The following nominal concentrations of the reference control 3,5-Dichlorophenol were tested on the same activated sludge and under identical conditions as in the case of the test item: 1.0, 3.2, 10.0 and 32.0 mg/L.
In comparison to the controls the inhibition of the respiration rate of the activated sludge was 75.48 % at the highest nominal concentration of 32.0 mg/L.
At the nominal concentrations of 1.0, 3.2 and 10.0 mg/L 18.97 %, 30.85 % and 37.41 % inhibition of the respiration rate was calculated respectively.
The 3-hour EC50 of 3,5-Dichlorophenol was calculated to be 10.79 mg/L with 95% confidence limits of 7.69 – 15.15 mg/L. - Conclusions:
- Under the conditions of this Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation) the observed endpoint for the effect of EPICLON EXA-7250 was the following:
The 3-hour EC50: > 1000 mg/L
Based on the results of this study, the test item EPICLON EXA-7250 had no inhibition effect on the total respiration rate of the microorganisms of the activated sludge, therefore further testing for nitrification inhibition is not necessary.
Reference
TEST ITEM
In comparison to the inoculum controls the inhibition of the respiration rate in the case of the activated sludge was between 0.73 % and 3.20 % in the examined range of 10 – 1000 mg/L of EPICLON EXA-7250 test item.
The average specific respiration rate (RS) of the untreated (Blank) controls (B1-B5) was 26.79 mg O2/L/h/g solid.
The variation coefficient of oxygen uptake rate in above untreated replicates was 2.21 %.
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
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