Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 942-582-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- transformation / dissolution of metals and inorganic metal compounds
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14-07-2017 to 11-09-2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Series on Testing and Assessment No. 29 (23-Jul-2001): Guidance document on transformation/dissolution of metals and metal compounds in aqueous media
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- flask method
- Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 97.1 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate
- Loading of aqueous phase:
- 100 other: mg/L of test item
- Incubation duration:
- 2 h
- Test conditions:
- - Reconstituted standard based on ISO 6341;
- The aqueous medium solution sterilized by filtration (0.2 µm)
- pH=7;
- 20 +/- 1 °C;
- dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 97.7 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate
- Loading of aqueous phase:
- 100 other: mg/L of test item
- Incubation duration:
- 6 h
- Test conditions:
- - Reconstituted standard based on ISO 6341;
- The aqueous medium solution sterilized by filtration (0.2 µm)
- pH=7;
- 20 +/- 1 °C;
- dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 97.5 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate
- Loading of aqueous phase:
- 100 other: mg/L of test item
- Incubation duration:
- 12 h
- Test conditions:
- - Reconstituted standard based on ISO 6341;
- The aqueous medium solution sterilized by filtration (0.2 µm)
- pH=7;
- 20 +/- 1 °C;
- dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 97.7 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate
- Loading of aqueous phase:
- 100 other: mg/L of test item
- Incubation duration:
- 24 h
- Test conditions:
- - Reconstituted standard based on ISO 6341;
- The aqueous medium solution sterilized by filtration (0.2 µm)
- pH=7;
- 20 +/- 1 °C;
- dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 96.9 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate
- Loading of aqueous phase:
- 100 other: mg/L of test item
- Incubation duration:
- 72 h
- Test conditions:
- - Reconstituted standard based on ISO 6341;
- The aqueous medium solution sterilized by filtration (0.2 µm)
- pH=7;
- 20 +/- 1 °C;
- dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 98.6 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate
- Loading of aqueous phase:
- 100 other: mg/L of test item
- Incubation duration:
- 168 h
- Test conditions:
- - Reconstituted standard based on ISO 6341;
- The aqueous medium solution sterilized by filtration (0.2 µm)
- pH=7;
- 20 +/- 1 °C;
- dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 54.1 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate; Calcium
- Loading of aqueous phase:
- 10 g/L
- Incubation duration:
- 0 h
- Test conditions:
- - Purified water adjusted to pH 2;
- 37 +/- 1°C;
- Dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 98.1 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate; Calcium
- Loading of aqueous phase:
- 10 g/L
- Incubation duration:
- 0.5 h
- Test conditions:
- - Purified water adjusted to pH 2;
- 37 +/- 1°C;
- Dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 99.3 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate; Calcium
- Loading of aqueous phase:
- 10 g/L
- Incubation duration:
- 2 h
- Test conditions:
- - Purified water adjusted to pH 2;
- 37 +/- 1°C;
- Dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 97.8 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate; Calcium
- Loading of aqueous phase:
- 10 g/L
- Incubation duration:
- 4 h
- Test conditions:
- - Purified water adjusted to pH 2;
- 37 +/- 1°C;
- Dark conditions. - Key result
- Type of test:
- full transformation/dissolution test - metals and sparingly soluble metal compounds
- Mean dissolved conc.:
- ca. 97.5 other: Total recovery (%)
- Element analysed:
- Sodium; Potassium; Sulphate; Calcium
- Loading of aqueous phase:
- 10 g/L
- Incubation duration:
- 6 h
- Test conditions:
- - Purified water adjusted to pH 2;
- 37 +/- 1°C;
- Dark conditions. - Conclusions:
- The transformation/dissolution of the test item in reconstituted standard water based on ISO6341 at pH 7 at 100 mg/L at 20± 1 °C has been determined by ion chromatography. The results demonstrate that the test item is effectively completely transformed/dissolved within 2 hours.
The transformation/dissolution of the test item in purified water adjusted to pH 2 at 10 g/L at 37 °C ± 1 °C has been determined by ion chromatography. The results demonstrate that the test item is effectively completely transformed/dissolved within 30 minutes. - Executive summary:
The transformation/dissolution of the test item has been determined using a GLP-compliant procedure designed to be compatible with Annex 10, Guidance on Transformation/Dissolution of Metals and Metal Compounds in Aqueous Media, OECD Environment, Health and Safety Publications, Series on Testing and Assessment, No.29, Environment Directorate,Organisationfor Economic Co-operation and Development, 23 July 2001.
The results are summarized below:
The transformation/dissolution of the test item in reconstituted standard water based on ISO6341 at pH 7 at 100 mg/L at 20 ± 1 °C has been determined by ion chromatography. The results of the analysis by ion chromatography are shown in the following table:
Shaking time at 20°C (hours)
Sodium
(g/kg)Potassium
(g/kg)Sulphate
(g/kg)Residue
(g/kg)Total recovery (%)
2
67.2
330
574
-
97.1
6
69.8
331
577
-
97.7
12
68.3
331
576
-
97.5
24
70.9
331
575
-
97.7
72
63.6
331
574
-
96.9
168
68.6
340
577
None detected
98.6
The results demonstrate that the test item is effectively completely transformed/dissolved within 2 hours into Na+, K+, SO4-- ions when added in ISO 6341 medium at pH 7 and 20°C (calcium was not analysed because its level in EXP was too low compared to the concentration of calcium in the tested medium).
The transformation/dissolution of the test item in pH 2 purified water at 10 g/L at 37 °C ± 1 °C has been determined by ion chromatography. The results of the analysis by ion chromatography are shown in the following table:
Shaking time at 37°C (hours)
Sodium
(g/kg)Potassium
(g/kg)Calcium
(g/kg)Sulphate (g/kg)
Residue
(g/kg)
Total recovery (%)
0
25.7
179
2.59
334
-
54.1
0.5
52.4
329
3.28
596
-
98.1
2
54.0
332
3.41
603
-
99.3
4
52.9
328
3.29
594
-
97.8
6
52.3
327
3.23
592
4.0
97.4
The results demonstrate that the test item is effectively completely transformed/dissolved within 30 minutes into Na+, K+, Ca++, SO4-- ions when added in purified water at pH 2 and 37°C.
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05-May-2010 to 16-Jun-2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of Signature: November 12, 2008
- Type of method:
- flask method
- Remarks:
- EXAMPLE: The determination was carried out using the flask method, Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008.
- Water solubility:
- 445.5 g/L
- Temp.:
- 20 °C
- pH:
- 5.23
- Conclusions:
- Interpretation of results : very soluble (> 10000 mg/L)
During this study the water solubility of Sodium Sulphate was determined and found to be 445.47 g/L at 20.0 °C and a pH of 5.23 using the flask shaking method. - Executive summary:
During this study the water solubility of Sodium Sulphate was determined. Vessels containing water and test item were shaken for 24 hours, 48 hours and 72 hours, respectively, at 30 °C. Afterwards the samples were equilibrated for another 24 hours at 20.0 °C. After dilution with water the concentrations in the aqueous solutions were determined by an IC-method.
The water solubility of Sodium Sulphate was determined to be
445.5 g/L at 20.0 °C and a pH of 5.23
using the flask shaking method.
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-04-12 to 2010-04-16
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Conducted to current accepted guidelines. No GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Deviations:
- yes
- Remarks:
- In place of centrifugation, the solution was filtered with filter paper prior to determination of the mass concentration of the substance in aqueous solution.
- GLP compliance:
- no
- Type of method:
- flask method
- Water solubility:
- 2.4 g/L
- Temp.:
- 20 °C
- pH:
- 7.7
- Details on results:
- In the first preliminary test, the sample remained essentially undissolved. At the end of the second preliminary test, the sample appeared totally dissolved. From the results of the preliminary testing, that the water solubility of calcium sulphate dihydrate was between 1 and 10 g per litre of water. In the definitive test, no tendency to concentration increase could be detected, so the average value of the 3 samples was taken to determine the solubility. It was observed that magnetic stirring increased the temperature slightly. During pH measurement, the temperature was measured from the filtrates allowing direct measurement during magnetic stirring. The temperature of the samples was found to increase from 20 to 21-22°C, which was not thought to produce noticeable effects on the solubility of the test material.
- Conclusions:
- Interpretation of results : soluble (1000-10000 mg/L)
The water solubility of CaSO4 was determined to be 2.4 g/L at 20°C and with a pH value of 7.7. - Endpoint:
- water solubility
- Type of information:
- other: handbook
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Data have been taken from many sources, including both compilations and the primary literature - where conflicts were found, the value deemed most reliable was chosen. The reference was peer-reviewed. In addition, the reference sources are indicated although not leading to sufficient information on the material & method used.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method followed unknown; data from handbook.
- GLP compliance:
- not specified
- Water solubility:
- 120 g/L
- Remarks on result:
- other: pH and T° not provided
- Conclusions:
- Interpretation of results : very soluble (> 10000 mg/L)
The water solubility of potassium sulfate is 120 g/L (no temperature). - Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- not specified
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Principles of method if other than guideline:
- No method specified
- GLP compliance:
- not specified
- Key result
- Water solubility:
- 120 g/L
- Conc. based on:
- test mat.
- Temp.:
- 25 °C
- Remarks on result:
- other: pH not provided
Referenceopen allclose all
2-RESULTS
2.1-TRANSFORMATION/DISSOLUTION AT PH 7
2.1.1-ION CHROMATOGRAPHY
The mean concentrations (mg/L, corrected for matrix blank), concentration per unit mass (mg/kg) and as a percentage recovery for the sodium, potassium and sulphate analyses are shown in the following tables
Sodium
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 2hr |
6.9337 |
68043.8 |
6.80 |
SPL 2 2hr |
7.1597 |
65624.8 |
6.56 |
SPL 3 2hr |
6.6097 |
67791.5 |
6.78 |
SPL 1 6hr |
7.3312 |
71944.7 |
7.19 |
SPL 2 6hr |
7.6312 |
69946.5 |
7.00 |
SPL 3 6hr |
6.5822 |
67509.4 |
6.75 |
SPL 1 12hr |
7.2342 |
70992.8 |
7.10 |
SPL 2 12hr |
7.2722 |
66656.0 |
6.67 |
SPL 3 12hr |
6.5552 |
67232.5 |
6.72 |
SPL 1 24hr |
7.2432 |
71081.1 |
7.11 |
SPL 2 24hr |
7.6817 |
70409.4 |
7.04 |
SPL 3 24hr |
6.9537 |
71319.7 |
7.13 |
SPL 1 72hr |
6.8797 |
67513.9 |
6.75 |
SPL 2 72hr |
6.5607 |
60134.4 |
6.01 |
SPL 3 72hr |
6.1462 |
63037.6 |
6.30 |
SPL 1 168hr |
7.6570 |
75142.0 |
7.51 |
SPL 2 168hr |
6.8207 |
62517.6 |
6.25 |
SPL 3 168hr |
6.6382 |
68083.8 |
6.81 |
Potassium
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 2hr |
33.8808 |
332491 |
33.25 |
SPL 2 2hr |
35.8413 |
328518 |
32.85 |
SPL 3 2hr |
32.0138 |
328347 |
32.83 |
SPL 1 6hr |
34.0973 |
334616 |
33.46 |
SPL 2 6hr |
35.8568 |
328660 |
32.87 |
SPL 3 6hr |
32.1033 |
329265 |
32.93 |
SPL 1 12hr |
34.0898 |
334542 |
33.45 |
SPL 2 12hr |
35.8863 |
328931 |
32.89 |
SPL 3 12hr |
32.0808 |
329034 |
32.90 |
SPL 1 24hr |
34.0133 |
333791 |
33.38 |
SPL 2 24hr |
35.8743 |
328821 |
32.88 |
SPL 3 24hr |
32.1443 |
329686 |
32.97 |
SPL 1 72hr |
34.2493 |
336107 |
33.61 |
SPL 2 72hr |
35.8843 |
328912 |
32.89 |
SPL 3 72hr |
32.0253 |
328465 |
32.85 |
SPL 1 168hr |
36.2023 |
355273 |
35.53 |
SPL 2 168hr |
35.8283 |
328399 |
32.84 |
SPL 3 168hr |
32.7953 |
336362 |
33.64 |
Sulphate
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 2hr |
58.8786 |
577807 |
57.78 |
SPL 2 2hr |
62.9819 |
577286 |
57.73 |
SPL 3 2hr |
55.2756 |
566929 |
56.69 |
SPL 1 6hr |
59.2475 |
581428 |
58.14 |
SPL 2 6hr |
62.9803 |
577271 |
57.73 |
SPL 3 6hr |
55.6540 |
570811 |
57.08 |
SPL 1 12hr |
59.1072 |
580051 |
58.01 |
SPL 2 12hr |
62.6056 |
573837 |
57.38 |
SPL 3 12hr |
55.9918 |
574275 |
57.43 |
SPL 1 24hr |
58.9411 |
578421 |
57.84 |
SPL 2 24hr |
62.5150 |
573003 |
57.30 |
SPL 3 24hr |
55.7930 |
572236 |
57.22 |
SPL 1 72hr |
59.1190 |
580167 |
58.02 |
SPL 2 72hr |
61.9963 |
568252 |
56.83 |
SPL 3 72hr |
55.9563 |
573911 |
57.39 |
SPL 1 168hr |
59.2752 |
581700 |
58.17 |
SPL 2 168hr |
61.6852 |
565400 |
56.54 |
SPL 3 168hr |
56.9305 |
583903 |
58.39 |
2.1.2-ICP-MS (confirmatory analysis)
From the analysis of the sample solutions, the counts per second for each analyte were interpolated from the respective calibrations to give the concentration of analyte (mg/L). The mean concentrations (mg/L), concentration per unit mass (mg/kg) and as a percentage recovery for the sodium, potassium and sulphate analyses are shown in the following tables:
Sodium
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 2hr |
7.17 |
70345 |
7.03 |
SPL 2 2hr |
8.59 |
78709 |
7.87 |
SPL 3 2hr |
7.44 |
76328 |
7.63 |
SPL 1 6hr |
8.24 |
80848 |
8.08 |
SPL 2 6hr |
7.44 |
68186 |
6.82 |
SPL 3 6hr |
7.41 |
75967 |
7.60 |
SPL 1 12hr |
7.78 |
76379 |
7.64 |
SPL 2 12hr |
7.65 |
70107 |
7.01 |
SPL 3 12hr |
9.11 |
93424 |
9.34 |
SPL 1 24hr |
8.36 |
82054 |
8.21 |
SPL 2 24hr |
9.12 |
83582 |
8.36 |
SPL 3 24hr |
8.47 |
86842 |
8.68 |
SPL 1 72hr |
10.2 |
100362 |
10.0 |
SPL 2 72hr |
8.87 |
81275 |
8.13 |
SPL 3 72hr |
8.39 |
86031 |
8.60 |
SPL 1 168hr |
10.1 |
99181 |
9.92 |
SPL 2 168hr |
8.53 |
78216 |
7.82 |
SPL 3 168hr |
8.08 |
82886 |
8.29 |
Potassium
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 2hr |
37.2 |
364925 |
36.5 |
SPL 2 2hr |
32.4 |
297010 |
29.7 |
SPL 3 2hr |
30.8 |
315478 |
31.6 |
SPL 1 6hr |
32.1 |
314940 |
31.5 |
SPL 2 6hr |
34.1 |
312152 |
31.2 |
SPL 3 6hr |
29.8 |
305441 |
30.5 |
SPL 1 12hr |
31.7 |
310825 |
31.1 |
SPL 2 12hr |
34.3 |
314660 |
31.5 |
SPL 3 12hr |
30.3 |
310682 |
31.1 |
SPL 1 24hr |
34.3 |
336110 |
33.6 |
SPL 2 24hr |
35.3 |
324221 |
32.4 |
SPL 3 24hr |
31.4 |
321639 |
32.2 |
SPL 1 72hr |
34.6 |
339226 |
33.9 |
SPL 2 72hr |
35.5 |
325232 |
32.5 |
SPL 3 72hr |
32.5 |
333472 |
33.4 |
SPL 1 168hr |
34.9 |
342158 |
34.2 |
SPL 2 168hr |
34.8 |
318893 |
31.9 |
SPL 3 168hr |
31.6 |
323864 |
32.4 |
Sulphate
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 2hr |
52.0 |
509811 |
51.0 |
SPL 2 2hr |
57.1 |
523100 |
52.3 |
SPL 3 2hr |
52.2 |
535301 |
53.5 |
SPL 1 6hr |
56.7 |
556379 |
55.6 |
SPL 2 6hr |
61.3 |
561922 |
56.2 |
SPL 3 6hr |
46.0 |
471789 |
47.2 |
SPL 1 12hr |
50.4 |
495081 |
49.5 |
SPL 2 12hr |
59.0 |
541034 |
54.1 |
SPL 3 12hr |
47.7 |
488835 |
48.9 |
SPL 1 24hr |
51.7 |
507445 |
50.7 |
SPL 2 24hr |
61.1 |
559708 |
56.0 |
SPL 3 24hr |
52.6 |
539425 |
53.9 |
SPL 1 72hr |
55.6 |
545592 |
54.6 |
SPL 2 72hr |
64.2 |
588460 |
58.9 |
SPL 3 72hr |
49.8 |
511104 |
51.1 |
SPL 1 168hr |
55.6 |
545856 |
54.6 |
SPL 2 168hr |
61.0 |
559220 |
55.9 |
SPL 3 168hr |
50.7 |
519631 |
52.0 |
2.1.3-DISCUSSION
The definitive results obtained by ion chromatography were assessed for reproducibility from the triplicate flasks for all time points. This demonstrated mean relative standard deviation of 4.06% for sodium, 1.50% for potassium, 0.99% forsulphate and therefore the test was considered to be consistent across the flasks at all the time points.
Comparison of the results obtained in the test with the theoretical concentrations from the Certificate of Analysis (sodium 66.0 g/kg, potassium 313 g/kg,sulphate564 g/kg), demonstrates that the test item was essentially fully dissolved in the test media at all time points.
The pH of the test solutions was stable (<0.2 pH unit deviation) over the duration of the study.
The dissolved oxygen level in all the test solutions was greater than 70% saturation (6.0 mg/L) and thus satisfied the guideline.
The calcium content of the solutions was not determined since there was a significant amount of calcium present in the test medium, and therefore it was considered that it would not be possible to obtain reliable results.
The ICP-MS confirmatory analysis demonstrated no significant difference in the levels of sodium, potassium andsulphate in the samples solutions.
The study results reported from the ion chromatography analysis were considered acceptable.
2.2-TRANSFORMATION/DISSOLUTION AT PH 2
2.2.1-ION CHROMATOGRAPHY
Sodium
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
0.236 |
2.31 x 104 |
2.31 |
SPL 2 0hr |
0.276 |
2.65 x 104 |
2.66 |
SPL 3 0hr |
0.285 |
2.75 x 104 |
2.75 |
SPL 1 0.5hr |
0.537 |
5.26 x 104 |
5.26 |
SPL 2 0.5hr |
0.546 |
5.26 x 104 |
5.26 |
SPL 3 0.5hr |
0.537 |
5.19 x 104 |
5.19 |
SPL 1 2hr |
0.590 |
5.78 x 104 |
5.78 |
SPL 2 2hr |
0.534 |
5.14 x 104 |
5.14 |
SPL 3 2hr |
0.547 |
5.29 x 104 |
5.29 |
SPL 1 4hr |
0.528 |
5.18 x 104 |
5.18 |
SPL 2 4hr |
0.550 |
5.30 x 104 |
5.30 |
SPL 3 4hr |
0.559 |
5.40 x 104 |
5.40 |
SPL 1 6hr |
0.541 |
5.30 x 104 |
5.30 |
SPL 2 6hr |
0.535 |
5.15 x 104 |
5.15 |
SPL 3 6hr |
0.541 |
5.23 x 104 |
5.23 |
Potassium
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
1.69 |
1.66 x 105 |
16.6 |
SPL 2 0hr |
1.97 |
1.90 x 105 |
19.0 |
SPL 3 0hr |
1.88 |
1.81 x 105 |
18.1 |
SPL 1 0.5hr |
3.36 |
3.29 x 105 |
32.9 |
SPL 2 0.5hr |
3.42 |
3.30 x 105 |
33.0 |
SPL 3 0.5hr |
3.40 |
3.29 x 105 |
32.9 |
SPL 1 2hr |
3.48 |
3.41 x 105 |
34.1 |
SPL 2 2hr |
3.40 |
3.28 x 105 |
32.8 |
SPL 3 2hr |
3.40 |
3.28 x 105 |
32.8 |
SPL 1 4hr |
3.34 |
3.27 x 105 |
32.7 |
SPL 2 4hr |
3.42 |
3.29 x 105 |
32.9 |
SPL 3 4hr |
3.38 |
3.27 x 105 |
32.7 |
SPL 1 6hr |
3.33 |
3.26 x 105 |
32.6 |
SPL 2 6hr |
3.43 |
3.30 x 105 |
33.0 |
SPL 3 6hr |
3.36 |
3.25 x 105 |
32.5 |
Calcium
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
2.60 x 10-2 |
2.55 x 103 |
0.255 |
SPL 2 0hr |
2.37 x 10-2 |
2.28 x 103 |
0.228 |
SPL 3 0hr |
3.04 x 10-2 |
2.94 x 103 |
0.294 |
SPL 1 0.5hr |
3.47 x 10-2 |
3.40 x 103 |
0.340 |
SPL 2 0.5hr |
3.41 x 10-2 |
3.29 x 103 |
0.329 |
SPL 3 0.5hr |
3.24 x 10-2 |
3.14 x 103 |
0.314 |
SPL 1 2hr |
3.71 x 10-2 |
3.64 x 103 |
0.364 |
SPL 2 2hr |
3.49 x 10-2 |
3.36 x 103 |
0.336 |
SPL 3 2hr |
3.33 x 10-2 |
3.22 x 103 |
0.322 |
SPL 1 4hr |
3.32 x 10-2 |
3.25 x 103 |
0.325 |
SPL 2 4hr |
3.48 x 10-2 |
3.35 x 103 |
0.335 |
SPL 3 4hr |
3.37 x 10-2 |
3.25 x 103 |
0.325 |
SPL 1 6hr |
3.08 x 10-2 |
3.01 x 103 |
0.301 |
SPL 2 6hr |
3.44 x 10-2 |
3.32 x 103 |
0.332 |
SPL 3 6hr |
3.47 x 10-2 |
3.35 x 103 |
0.335 |
Sulphate
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
3.18 |
3.11 x 105 |
31.1 |
SPL 2 0hr |
3.65 |
3.52 x 105 |
35.2 |
SPL 3 0hr |
3.51 |
3.39 x 105 |
33.9 |
SPL 1 0.5hr |
6.08 |
5.96 x 105 |
59.6 |
SPL 2 0.5hr |
6.20 |
5.97 x 105 |
59.7 |
SPL 3 0.5hr |
6.15 |
5.95 x 105 |
59.5 |
SPL 1 2hr |
6.31 |
6.18 x 105 |
61.8 |
SPL 2 2hr |
6.19 |
5.96 x 105 |
59.6 |
SPL 3 2hr |
6.16 |
5.95 x 105 |
59.5 |
SPL 1 4hr |
6.05 |
5.93 x 105 |
59.3 |
SPL 2 4hr |
6.19 |
5.96 x 105 |
59.6 |
SPL 3 4hr |
6.14 |
5.94 x 105 |
59.4 |
SPL 1 6hr |
6.04 |
5.92 x 105 |
59.2 |
SPL 2 6hr |
6.16 |
5.93 x 105 |
59.3 |
SPL 3 6hr |
6.11 |
5.91 x 105 |
59.1 |
2.2.2-ICP-MS (confirmatory analysis)
From the analysis of the sample solutions, the counts per second for each analyte were interpolated from the respective calibrations to give the concentration of analyte (mg/L). The mean concentrations (g/L), concentration per unit mass (mg/kg) and percentage recovery for the sodium, potassium, calcium and sulphate analyses are shown in the following tables:
Sodium
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
0.401 |
3.93 x 104 |
3.93 |
SPL 2 0hr |
0.547 |
5.27 x 104 |
5.27 |
SPL 3 0hr |
0.369 |
3.56 x 104 |
3.56 |
SPL 1 0.5hr |
0.596 |
5.84 x 104 |
5.84 |
SPL 2 0.5hr |
1.04 |
1.00 x 105 |
10.0 |
SPL 3 0.5hr |
0.917 |
8.87 x 104 |
8.87 |
SPL 1 2hr |
0.707 |
6.92 x 104 |
6.92 |
SPL 2 2hr |
1.01 |
9.78 x 104 |
9.78 |
SPL 3 2hr |
0.971 |
9.39 x 104 |
9.39 |
SPL 1 4hr |
0.663 |
6.50 x 104 |
6.50 |
SPL 2 4hr |
1.11 |
1.07 x 105 |
10.7 |
SPL 3 4hr |
0.844 |
8.16 x 104 |
8.16 |
SPL 1 6hr |
0.721 |
7.07 x 104 |
7.07 |
SPL 2 6hr |
1.06 |
1.02 x 105 |
10.2 |
SPL 3 6hr |
0.963 |
9.31 x 104 |
9.31 |
Potassium
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
2.10 |
2.06 x 105 |
20.6 |
SPL 2 0hr |
2.73 |
2.63 x 105 |
26.3 |
SPL 3 0hr |
2.70 |
2.61 x 105 |
26.1 |
SPL 1 0.5hr |
3.75 |
3.67 x 105 |
36.7 |
SPL 2 0.5hr |
4.82 |
4.64 x 105 |
46.4 |
SPL 3 0.5hr |
4.89 |
4.73 x 105 |
47.3 |
SPL 1 2hr |
3.82 |
3.75 x 105 |
37.5 |
SPL 2 2hr |
4.57 |
4.40 x 105 |
44.0 |
SPL 3 2hr |
4.93 |
4.77 x 105 |
47.7 |
SPL 1 4hr |
3.77 |
3.70 x 105 |
37.0 |
SPL 2 4hr |
5.24 |
5.05 x 105 |
50.5 |
SPL 3 4hr |
5.02 |
4.85 x 105 |
48.5 |
SPL 1 6hr |
3.94 |
3.86 x 105 |
38.6 |
SPL 2 6hr |
4.72 |
4.55 x 105 |
45.5 |
SPL 3 6hr |
4.99 |
4.82 x 105 |
48.2 |
Calcium
Sample |
Meanconcentration (g/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
2.51 x 10-2 |
2.46 x 103 |
0.246 |
SPL 2 0hr |
2.44 x 10-2 |
2.35 x 103 |
0.235 |
SPL 3 0hr |
2.48 x 10-2 |
2.40 x 103 |
0.240 |
SPL 1 0.5hr |
3.28 x 10-2 |
3.22 x 103 |
0.322 |
SPL 2 0.5hr |
4.07 x 10-2 |
3.93 x 103 |
0.393 |
SPL 3 0.5hr |
3.61 x 10-2 |
3.49 x 103 |
0.349 |
SPL 1 2hr |
3.63 x 10-2 |
3.56 x 103 |
0.356 |
SPL 2 2hr |
3.90 x 10-2 |
3.76 x 103 |
0.376 |
SPL 3 2hr |
3.47 x 10-2 |
3.35 x 103 |
0.335 |
SPL 1 4hr |
3.31 x 10-2 |
3.25 x 103 |
0.325 |
SPL 2 4hr |
3.73 x 10-2 |
3.60 x 103 |
0.360 |
SPL 3 4hr |
3.10 x 10-2 |
3.00 x 103 |
0.300 |
SPL 1 6hr |
3.48 x 10-2 |
3.41 x 103 |
0.341 |
SPL 2 6hr |
4.24 x 10-2 |
4.09 x 103 |
0.409 |
SPL 3 6hr |
3.51 x 10-2 |
3.39 x 103 |
0.339 |
Sulphate
Sample |
Meanconcentration (mg/L) |
Meanconcentration (mg/kg) |
Recovery (%) |
SPL 1 0hr |
2.60 x 103 |
2.55 x 105 |
25.5 |
SPL 2 0hr |
3.34 x 103 |
3.27 x 105 |
32.7 |
SPL 3 0hr |
3.40 x 103 |
3.33 x 105 |
33.3 |
SPL 1 0.5hr |
4.68 x 103 |
4.58 x 105 |
45.8 |
SPL 2 0.5hr |
5.58 x 103 |
5.47 x 105 |
54.7 |
SPL 3 0.5hr |
5.46 x 103 |
5.35 x 105 |
53.5 |
SPL 1 2hr |
4.52 x 103 |
4.42 x 105 |
44.2 |
SPL 2 2hr |
5.65 x 103 |
5.54 x 105 |
55.4 |
SPL 3 2hr |
5.66 x 103 |
5.55 x 105 |
55.5 |
SPL 1 4hr |
4.65 x 103 |
4.56 x 105 |
45.6 |
SPL 2 4hr |
5.44 x 103 |
5.33 x 105 |
53.3 |
SPL 3 4hr |
5.47 x 103 |
5.36 x 105 |
53.6 |
SPL 1 6hr |
4.57 x 103 |
4.48 x 105 |
44.8 |
SPL 2 6hr |
5.76 x 103 |
5.64 x 105 |
56.4 |
SPL 3 6hr |
6.16 x 103 |
6.04 x 105 |
60.4 |
2.2.3-DISCUSSION
The definitive results obtained by ion chromatography were assessed for reproducibility from the triplicate flasks for all time points. This demonstrated mean relative standard deviations of 3.89% for sodium, 2.07% for potassium, 6.13% for calcium and 1.80% for sulphate. Sodium, potassium and sulphate analysis were considered to be consistent (<5% RSD) across the flasks at all the time points. The higher variability in the calcium results was considered to be due to lower concentrations present in the sample solutions.
Comparison of the results obtained in the test with the theoretical concentrations from the Certificate of Analysis (sodium 66.0 g/kg, potassium 313 g/kg, sulphate 564 g/kg, calcium 0.285 g/kg), demonstrates that the test item was essentially fully dissolved in the test media after 30 minutes.
Although the ICP-MS confirmatory analysis demonstrated minor differences in reported concentrations, the analysis served to confirm the analyte species and approximate levels in the sample solutions.
For the analysis by IC, matrix blanks were demonstrated generally low and inconsistent peak areas, therefore it was not considered necessary to correct the sample results for the contribution from the matrix blanks. In the ICP-MS analysis, matrix blanks were significant for sulphur(sulphate analysis) and therefore matrix blank corrections were applied to all ICP-MS analysis.
At each time point from 0.5 hour onwards, all of the samples were observed to be clear colorless and free from excess undissolved test item.
The pH of all the test solutions increased after addition of test item to pH 2.3 and remained stable throughout the duration of the test.
The dissolved oxygen level in all the test solutions was greater than 70% saturation (6.0 mg/L) and thus satisfied the guideline.
The saturation concentration of Sodium Sulphate at room temperature was estimated by a simplified flask method in the preliminary test to be higher than 10-2g/L. Therefore, the flask shaking test was performed.
After a shaking time of 24 hours, 48 hours and 72 hours, respectively, at 30 °C, the samples were equilibrated for another 24 hours at 20 °C. Afterwards, the samples were centrifuged, diluted and analyzed by IC. The water solubility of Sodium Sulphate at 20.0 °C was determined to be 445.47 g/L. The measured results differ by less than 15%. Therefore, the study is considered to be valid. The results of the individual measurements of the main test are summarized inTable 2. The tabulated values represent rounded results, which were obtained by calculation using the exact raw data.
A typical chromatogram of a standard solution is given in Figure 1. A typical chromatogram of a sample solution after 72 hours of incubation is shown in Figure 2. An IC chromatogram of a blank solution is shown in Figure 3. The calibration curve and data are shown in Figure 4 and in Table 3. The r² fit was 0.9999 (optimum 1.0000). This reflects the linearity of the IC-system within the calibration range of 3.324 µg/mL to 99.72 µg/mL of the test item.
Table 1 Data of the Performance of the Water Solubility Test
Sample Solution No. |
1 |
2 |
3 |
4 |
5 |
6 |
|||||||
Weighed sample of the test item [g] |
7.883 |
7.340 |
8.485 |
7.495 |
8.007 |
7.647 |
|||||||
Added volume of water [mL] |
10 |
10 |
10 |
10 |
10 |
10 |
|||||||
Agitation time at 30 °C [hours] |
72 |
72 |
48 |
48 |
24 |
24 |
|||||||
Equilibration time at 20 °C [hours] |
24 |
24 |
24 |
24 |
24 |
24 |
|||||||
pH value of test item solution |
5.16 |
5.17 |
5.21 |
5.24 |
5.31 |
5.31 |
|||||||
Dilution factor before quantification |
10000 |
10000 |
10000 |
10000 |
10000 |
10000 |
Table 2 Results of the Water Solubility of Sodium Sulphate
Sample No. |
Shaking time at 30 °C |
Concentration analyzed |
Concentration calculated |
Water solubility calculated (rounded) |
|||||
|
|
[mg/L] |
[g/L] |
[g/L] |
|||||
1 |
72 |
45.000 |
450.001 |
445.47 SD = 14.81 |
|||||
2 |
45.788 |
457.876 |
|||||||
3 |
48 |
44.821 |
448.206 |
||||||
4 |
45.075 |
450.754 |
|||||||
5 |
24 |
44.994 |
449.935 |
||||||
6 |
41.603 |
416.031 |
The saturation mass concentration is calculated from the bold values
SD = standard deviation
Table 1: tabulated water solubility data
Sample |
Ca in g/L |
SO4 in g/L |
pH |
||
Measurements |
Mean value |
Measurements |
Mean value |
||
Flask 1 |
0.724 |
0.725 |
1.911 |
1.9 |
7.7 |
0.729 |
1.913 |
7.7 |
|||
0.723 |
1.9 |
7.6 |
|||
Flask 2 |
0.731 |
0.728 |
1.873 |
1.86 |
7.7 |
0.723 |
1.854 |
7.6 |
|||
0.729 |
1.881 |
7.6 |
|||
Flask 3 |
0.724 |
0.723 |
1.735 |
1.73 |
7.7 |
0.724 |
1.724 |
7.6 |
|||
0.721 |
1.719 |
7.7 |
The water solubility was determined from the concentration of calcium as the value calculated by using the solubility of SO4 instead of Ca produces a slightly higher value, this is due to a small interference by small quantities of sodium, potassium, magnesium sulfates explaining this small overestimation.
Value given in the reference: 1 g/8.3 mL No data on temperature.
Description of key information
The transformation/dissolution of the target substance "Sulfates of potassium, sodium and calcium, by-product from fermentation" in reconstituted standard water (ISO6341) at pH 7, at 100 mg/L, and at 20± 1 °C has been determined by ion chromatography. The results demonstrate that the test item is effectively completely transformed/dissolved within 2 hours into sulfate anions and potassium, sodium and calcium cations.
The water solubility of "Sulfates of potassium, sodium and calcium, by-product from fermentation" is 2.4 g/L at 20°C (pH=7.7), which is the lowest water solubility measured on the 3 source substances, Potassium sulfate (CAS 7778 -80 -5), Sodium sulfate (CAS 7757 -82-6) and Calcium sulfate (CAS 7778-18-9).
.
Key value for chemical safety assessment
- Water solubility:
- 2.4 g/L
- at the temperature of:
- 20 °C
Additional information
The transformation/dissolution of the target substance "Sulfates of potassium, sodium and calcium, by-product from fermentation" in reconstituted standard water (ISO6341), at pH 7, at 100 mg/L and at 20± 1°C, has been determined according to the Guidance on Transformation/Dissolution of Metals and Metal Compounds in Aqueous Media (OECD Series on Testing and Assessment, No.29, 23 July 2001). The results demonstrate that the test item is effectively completely transformed/dissolved within 2 hours into sulfate anions and potassium, sodium and calcium cations, measured by by ion chromatography.
As the 3 source substances, Potassium sulfate (CAS 7778-80-5), Sodium sulfate (CAS 7757-82-6) and Calcium sulfate (CAS 7778-18-9) are also completely dissociated in aqueous media into sulfate anions and the respective cations, then the water solubility of the target substance can be assessed through the water solubility of the source substances which are : 120g/L at 25°C (pH not specified) for potassium sulfate, 445 g/L at 20°C (pH=5.23) for sodium sulfate and 2.4 g/L at 20°C (pH=7.7) for calcium sulfate. The water solubility of "Sulfates of potassium, sodium and calcium, by-product from fermentation" is 2.4 g/L at 20°C (pH=7.7), which is the lowest water solubility observed amongst the 3 source substances.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.