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EC number: 700-316-5 | CAS number: 1155405-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 199-01-25 to 1999-01-29
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tri (hexyl, octyl, decyl) citrate
- IUPAC Name:
- Tri (hexyl, octyl, decyl) citrate
- Reference substance name:
- -
- EC Number:
- 430-290-8
- EC Name:
- -
- IUPAC Name:
- 430-290-8
- Reference substance name:
- 2-Hydroxypropane-1,2,3-tricarboxylic acid, tri (hexyl, octyl, decyl) ester
- IUPAC Name:
- 2-Hydroxypropane-1,2,3-tricarboxylic acid, tri (hexyl, octyl, decyl) ester
- Details on test material:
- - Name of test material (as cited in study report): Tri (hexyl, octyl, decyl) citrate
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: in water for at least 2 hours
- Storage condition of test material: in original container, at room temperature, in the dark
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: -29°C (freezing point: -35.5°C)
- Boiling point: test substance showed decomposition above 260°C
- Vapour pressure: 8.5 x 10E-3 p at 25°C
- Water solubility (under test conditions): < 1 mg/L
- Solubility in organic solvents: soluble
- log Pow: > 6
- pKa: not applicable
- Base or acid catalysis of test material: no data
- UV absorption: no data
- Stability of test material at room temperature: stable
- pH dependance on stability: no data
OTHER PROPERTIES (if relevant for this endpoint)
- Adsorption characteristics: no data
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- In a pre-experiment (without GLP) the dissolved organic carbon contents (DOC) of the test substance solution (same preparation as in the test) was determined. In the solution the same amount of DOC was determined as in the blank control. Thus, no dissolved test substance could be verified in test water. Therefore, no analytical dose verification in the test media of the acute toxicity test with fish could be done.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A supersaturated stock suspension of the test substance with a nominal concentration of 100 mg/L was prepared by weighing 503 mg of the test substance into 5 L test water, following ultrasonic treatment for 10 minutes and intense stirring. The supersaturated stock suspension was stirred on a magnetic stirrer at room temperauter in the dark over 3 days . Thereafter the suspension was filtered through a cellulose-nitrate filter with a defined pore size of 0.2 µm just before the start of the test.
- Controls: reconstituted water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no, suspension was filtered
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: Brachydanio rerio
- Source: City Zoo, Darmstadt, Germany
- Age at study initiation (mean and range, SD): juveniles (no further details mentioned)
- Length at study initiation (length definition, mean, range and SD): 3.11 ± 0.16 cm (mean of 10 fish from the test fish batch)
- Weight at study initiation (mean and range, SD): 0.27 ± 0.06 g (mean of 10 fish from the test fish batch)
- Method of breeding: not mentioned
- Feeding during test: none
ACCLIMATION
- Acclimation period: 7 weeks
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: TETRA MIN Hauptfutter, TETRA-Werke, Melle, Germany (commercial fish diet)
- Feeding frequency: not mentioned
- Health during acclimation (any mortality observed): no fish died and all fish were healthy
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 2.5 mmol/L (= 250.0 mg/L) as CaCO3
- Test temperature:
- 21 - 23 °C
- pH:
- 7.6 - 7.9
- Dissolved oxygen:
- 8.1 - 8.9 mg/L
- Nominal and measured concentrations:
- filtrated WAF
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 9 L glass aquaria with 5 L test medium
- Aeration: slightly aerated during test
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.378 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1 (based on molarity)
- Conductivity: not mentioned
- Culture medium different from test medium: no
- Intervals of water quality measurement: not mentioned
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light
- Light intensity: 712 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The test fish were observed after approx. 2, 24, 48, 72 and 96 hours test duration for symptoms of intoxication and mortality. The water temperature, pH-values and the dissolved oxygen concentrations were determined daily in test concentration and control. The behaviour of the test substance in test water was determined once every day during the test. - Reference substance (positive control):
- no
Results and discussion
- Details on results:
- - Behavioural abnormalities: not observed
- Observations on body length and weight: not made
- Other biological observations: no signs of intoxication were observed
- Mortality of control: no mortality occured
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No remarkable observations were made concerning the behaviour of the test substance in the test medium
- Effect concentrations exceeding solubility of substance in test medium: not applicable, filtrated test solution - Reported statistics and error estimates:
- The NOEC and the LC0 were determined directly from the raw data. The LOEC, the LC50 and the LC100 could not be quantified due to the absence of toxicity of the test substance.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table: Mortality and symptoms of intoxication
Treatment Number of fish with intoxication symptoms and intoxication symtpoms / number of dead fish / number of fish tested after 2 h 24 h 48 h 72 h 96 h control 0 / 0 / 7 0 / 0 / 7 0 / 0 / 7 0 / 0 / 7 0 / 0 / 7 filtrate of test substance 0 / 0 / 7 0 / 0 / 7 0 / 0 / 7 0 / 0 / 7 0 / 0 / 7 Possible intoxication symptoms with abbreviations: strongly extended gills (AK), apathy (AP), distended abdomen (BA), fins frayed out at the border (FV), Exopthalmus (GA), convulsions (KR), fish mainly at the water surface (OB), mucus secretion (SA), fish lying on side of back (SR), strong ventilation (SV), tumbling during swimming (TS)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- no fish died in the control and oxygen saturation was always > 60%
- Conclusions:
- Due to low water solubility limit of the test substance a filtrate of a supersaturated stock suspension of nominal 100 mg/L was tested. Thus no concentrations above the solubility limit of the test substance in the used test water were tested. Due to the low water solubility limit no analytical dose verfication could be done in the present test. Therefore, the biological results could not be related to a specific concentration but to the solubility limit of Tri (hexyl, octyl, decyl) citrate in the test medium.
In the cotnrol and in the undiluted filtrate of the supersaturated stock suspension all fishes survived until the end of the test and no signs of intoxication were observed. Therefore, the 96-hour NOEC and the 96-hour LC0 of Tri (hexyl, octyl, decyl) citrate to Zebra fish were determined to be at least up to the solubility limit. The NOEC and the LC0 might even be higher, but concentrations in excess of the solubility limit had not been tested. The 96-hour LOEC, the 96-hour LC50 and the 96-hour LC100 were clearly higher than the solubility limit of the test substance in the used test water.
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