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EC number: 604-638-6 | CAS number: 148520-82-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
No experimental study to investigate skin or eye irritation potential of the target substance is available. Data generated with the supporting substance LABS Na is used to cover this endpoint. In the key, in vivo skin irritation study in rabbits (performed according to OECD guideline 404), the test substance is irritant to the skin. In a key, reliable in vivo eye irritation study (performed according to OECD guideline 405), the test substance was demonstrated to cause irreversible effects to the eye and thus is classified as category 1.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 18, 1986 - March 31, 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- GLP compliance:
- no
- Specific details on test material used for the study:
- Alkylbenzene sulfonate, sodium salt (designated as P-500 N-Na). Activity 47%. Average alkyl chain length = C11.2. Clear yellow liquid stored at ambient temperature.
- Species:
- rabbit
- Strain:
- other: New Zealand albino
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Froxfield Rabbits and Buckmasters
- Age at study initiation: 12-14 weeks
- Weight at study initiation: 2.7-3.3 kg
- Housing: individually housed in metal cages, identified by aluminum ear tags
- Diet (e.g. ad libitum): SDS Standard Rabbit Diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation: yes
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 degree C
- Humidity (%): 30-70
- Air changes (per hr): 19
- Photoperiod (hrs dark / hrs light): 12 hrs light/12 hrs dark
IN-LIFE DATES: From: March 18, 1986 To: March 31, 1986 - Type of coverage:
- semiocclusive
- Preparation of test site:
- other: clipped
- Vehicle:
- not specified
- Controls:
- not specified
- Amount / concentration applied:
- 0.5 ml aliquot of P-500 N-Na
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 14 days
- Number of animals:
- 3
- Details on study design:
- Hair was clipped from a 10 x 10 area of the dorsal-lumbar region. Test substance was applied under a gauze pad, and then covered with elastic adhesive dressing. Examination of the treated skin was made approximately 30 minutes after removal of the patch and daily through 14 days. Grading and scoring of the dermal reactions was performed using the standard numerical scoring system.
- Irritation parameter:
- primary dermal irritation index (PDII)
- Basis:
- mean
- Time point:
- other: 24, 48, and 72 hrs
- Score:
- 2.17
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- erythema score
- Basis:
- animal: 1417
- Time point:
- 24/48/72 h
- Score:
- 2.67
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- edema score
- Basis:
- animal: 1417
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- erythema score
- Basis:
- animal: 1491
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 3
- Reversibility:
- fully reversible within: 11 days
- Irritation parameter:
- edema score
- Basis:
- animal: 1491
- Time point:
- 24/48/72 h
- Score:
- 1.67
- Max. score:
- 3
- Reversibility:
- fully reversible within: 10 days
- Irritation parameter:
- erythema score
- Basis:
- animal: 1726
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 14 days
- Irritation parameter:
- edema score
- Basis:
- animal: 1726
- Time point:
- 24/48/72 h
- Score:
- 2.67
- Max. score:
- 3
- Reversibility:
- fully reversible within: 12 days
- Irritant / corrosive response data:
- Well defined to moderate skin reactions were observed in all three animals following removal of the bandages. Desquamation of the stratum corneum was observed in all three animals. The reaction in all three animals gradually ameliorated from Days 5, 10 and 11, respectively, and had all resolved completely in one animal by Day 12.
- Interpretation of results:
- Category 2 (irritant)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test substance was moderately to severely irritating to skin, but effects were not fully reversible within 14 days. The test substance is a Category 2 irritant according to EU GHS guidelines based on persistent irritation.
- Executive summary:
This study examined the potential for the test substance to cause dermal irritation. Three rabbits were exposed to 0.5 ml of the test substance dermally for 4 hrs. The test substance was covered with an occlusive dressing and removed by washing 4 hrs later. Animals were examined at 30 minutes after removal, and daily thereafter for 14 days. Irritation was noted in all animals at the first observation (maximum score of 2). Symptoms worsened, and desquamation, necrosis, and hyperkeratinization was noted by day 4. Symptoms resolved in one animals by day 12, but in the other two animals, symptoms were seen through the end of the observation period. The primary dermal irritation index was 2.17. The test substance is considered a Category 2 irritant according to EU GHS guidelines based on persistent irritation.
- Endpoint:
- skin irritation: in vitro / ex vivo
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- an in vitro skin irritation study does not need to be conducted because adequate data from an in vivo skin irritation study are available
Referenceopen allclose all
Results of Skin Irritation Study
Day |
Animal |
|||||
1417 |
1491 |
1726 |
||||
Erythema |
Edema |
Erythema |
Edema |
Erythema |
Edema |
|
0 |
2 |
1 |
2 |
2 |
2 |
1 |
1 |
2 |
2 |
2 |
3 |
2 |
3 |
2 |
2 |
2 |
2 |
1 |
2 |
2B |
3 |
4 |
2A |
2 |
1C |
2 |
3B |
4 |
4 |
2A |
1 |
1C |
2 |
3B |
5 |
4 |
2A |
1 |
1C |
2 |
3B |
6 |
4 |
3A |
1 |
1C |
2 |
3B |
7 |
4 |
3A |
1 |
1C |
2 |
2B |
8 |
4A |
3C |
1 |
1C |
2 |
2B |
9 |
2 |
2 |
1 |
1C |
2B |
2B |
10 |
1 |
2 |
1 |
0 |
1 |
2 |
11 |
1 |
1 |
0 |
0 |
1 |
2 |
12 |
1 |
1 |
- |
- |
1 |
0 |
13 |
1 |
1 |
- |
- |
1 |
0 |
A: Necrosis B : Hyperkeratinization C : Desquamation
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 24, 1986 - May 12, 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Froxfield Rabbits and Buckmasters
- Age at study initiation: 9-13 weeks
- Weight at study initiation: 2.1-3.1 kg
- Housing: individually housed in metal cages, identified by aluminum ear tags
- Diet (e.g. ad libitum): SDS Standard Rabbit Diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation: yes
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 °C
- Humidity (%): 30-70%
- Air changes (per hr): 19
- Photoperiod (hrs dark / hrs light): 12 hrs light/12 hrs dark
IN-LIFE DATES: From: March 24, 1986 To: May 12, 1986 - Vehicle:
- unchanged (no vehicle)
- Controls:
- not specified
- Amount / concentration applied:
- 0.1 mL aliquot of P-500 N-Na
- Duration of treatment / exposure:
- For three rabbits the eyelids were then gently held together for one second before releasing.
For three other rabbits the eyes were irrigated with water for 5 minutes following a 4-second exposure.
For the remaining three rabbits the eyes were irrigated for 5 minutes following a 30-second exposure. - Observation period (in vivo):
- Eyes were examined after 1 hour and 1, 2, 3, 4, 7, 14 and 21 days after exposure.
- Number of animals or in vitro replicates:
- 9
- Details on study design:
- SCORING SYSTEM: Draize scale
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Remarks:
- animal 1
- Time point:
- other: 24, 48, and 72 hrs
- Score:
- 1.75
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 21 days
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Remarks:
- animal 2
- Time point:
- other: 24, 48, and 72 hrs
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: rinse after 4 seconds
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Remarks:
- animal 3
- Time point:
- other: 24, 48, and 72 hrs
- Score:
- 1.06
- Max. score:
- 2
- Reversibility:
- fully reversible within: 14 days
- Remarks on result:
- other: rinsed after 30 seconds
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2.33
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1.67
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.67
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: rinsed after 4 sec
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 2.33
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: rinsed after 4 sec
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.67
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: rinsed after 4 sec
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0.33
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: rinsed after 30 sec
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: rinsed after 30 sec
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1.33
- Max. score:
- 4
- Reversibility:
- not specified
- Remarks on result:
- other: rinsed after 30 sec
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1.33
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 3
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 3
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 1.67
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not specified
- Remarks on result:
- other: not rinsed
- Irritant / corrosive response data:
- The following results were noted:
1) Three animals without any rinsing: averaged irritation scores (24, 48, 72 hours) for each animal: cornea 2.3, 1.7, 2; iris: 1.3, 0, 0; conjunctivae redness: 3, 1.7, 2; conjunctivae chemosis: 3, 2, 2. In the first animal the effects were persistent at day 21.
2) Three animals with rinsing for five minutes following a 30 second exposure: averaged scores: cornea 0.7, 1, 1.3; iris: 0, 0.7, 0.3; conjunctivae redness: 1.7, 2, 1.3; conjunctivae chemosis: 2, 1.3, 2. The eyes were normal 7 or 14 days after instillation.
3) Three animals with rinsing for five minutes following a 4 second exposure: averaged scores: cornea 0.7, 2.3, 0.7; iris: 0, 0, 0; conjunctivae redness: 1.7, 1.7, 1; conjunctivae chemosis: 1.3, 2, 1. The eyes were normal 7 days after instillation.
Findings lead to a definition of irritating for LAS at 47% applied without rinsing, irritating (even if with lower effects, mainly as cornea opacity and conjunctivae redness) with rinsing after 30 second of exposure, and not irritating with rinsing after 4 second of exposure. - Interpretation of results:
- Category 1 (irreversible effects on the eye)
- Conclusions:
- When not rinsed, damage to the eyes due to the test substance was not reversed within 21 days. According to EU GHS guidelines, the test substance is a category 1 eye irritant.
- Executive summary:
This study examined the potential of the test substance to cause irritation to eyes. Groups of three rabbits had 0.1 ml of test substance placed in each of their eyes. In one group, the eyes were not rinsed. In the second group, the eyes were rinsed after 4 seconds of exposure. In the third group, the eyes were rinsed after 30 seconds of exposure. Observations were made one hour and 1, 2, 3, 4, 7, 14, and 21 days after exposure. Severe irritation was noted in the animals whose eyes were not rinsed. This irritation was not resolved in one of these animals at the end of 21 days. Irritation was also seen in the animals rinsed after 30 seconds, although the irritation was not as severe, and the effects were fully reversible within 14 days. Mild irritation was seen in the animals rinsed after 4 seconds. These effects were fully reversible within 7 days. Since OECD guideline 405 for eye irritation studies calls for an exposure of at least 24 hrs, the results for unrinsed eyes were used for classification. According to these results, the test substance is a category 1 eye irritant because it caused damage that was not reversible within 21 days.
- Endpoint:
- eye irritation: in vitro / ex vivo
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- an in vitro eye irritation study does not need to be conducted because adequate data from an in vivo eye irritation study are available
Referenceopen allclose all
Results of Eye Irritation Study : No Rinsing
Hours |
Days |
||||||||
Animal |
1 |
24 |
48 |
72 |
4 |
7 |
14 |
21 |
|
1 |
Cornea |
2 |
1 |
2 |
4 |
3B |
3B |
3BC |
3BC |
Iris |
0 |
1 |
1 |
2 |
1 |
1 |
1 |
0 |
|
Conjunctiva - Erythema |
1 |
3 |
3 |
3 |
3 |
3 |
2 |
1 |
|
Conjunctiva - Chemosis |
2 |
3 |
3 |
3 |
3 |
2 |
2 |
1 |
|
2 |
Cornea |
1 |
1 |
2 |
2 |
2 |
1 |
0 |
|
Iris |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
||
Conjunctiva - Erythema |
1 |
1 |
2 |
2 |
1 |
0 |
0 |
||
Conjunctiva - Chemosis |
1 |
2 |
2 |
2 |
1 |
0 |
0 |
||
3 |
Cornea |
1 |
2 |
2 |
2 |
2 |
1 |
0 |
|
Iris |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
||
Conjunctiva - Erythema |
1 |
2 |
2 |
2 |
2 |
1 |
0 |
||
Conjunctiva - Chemosis |
1 |
2 |
2 |
2 |
2 |
0 |
0 |
Results of Eye Irritation Study : Rinsing after 4 Seconds
Hours |
Days |
||||||
Animal |
1 |
24 |
48 |
72 |
4 |
7 |
|
1 |
Cornea |
0 |
0 |
1 |
1 |
1 |
0 |
Iris |
0 |
0 |
0 |
0 |
1 |
0 |
|
Conjunctiva - Erythema |
1 |
2 |
2 |
1 |
2 |
0 |
|
Conjunctiva ¿ Chemosis |
2 |
2 |
1 |
1 |
1 |
0 |
|
2 |
Cornea |
0 |
2 |
3 |
2 |
0 |
0 |
Iris |
0 |
0 |
0 |
0 |
0 |
0 |
|
Conjunctiva - Erythema |
1 |
2 |
2 |
1 |
2 |
0 |
|
Conjunctiva - Chemosis |
2 |
2 |
2 |
1 |
1 |
0 |
|
3 |
Cornea |
0 |
1 |
0 |
1 |
1 |
0 |
Iris |
0 |
0 |
0 |
0 |
0 |
0 |
|
Conjunctiva - Erythema |
1 |
1 |
1 |
1 |
2 |
0 |
|
Conjunctiva - Chemosis |
2 |
1 |
1 |
1 |
1 |
0 |
Results of Eye Irritation Study : Rinsing after 30 Seconds
Hours |
Days |
|||||||
Animal |
1 |
24 |
48 |
72 |
4 |
7 |
14 |
|
1 |
Cornea |
0 |
1 |
0 |
D |
D |
1 |
0 |
Iris |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
Conjunctiva - Erythema |
1 |
2 |
2 |
1 |
1 |
1 |
0 |
|
Conjunctiva - Chemosis |
2 |
1 |
1 |
0 |
0 |
0 |
0 |
|
2 |
Cornea |
0 |
1 |
1 |
1 |
2 |
0 |
|
Iris |
0 |
0 |
1 |
1 |
0 |
0 |
||
Conjunctiva - Erythema |
1 |
2 |
2 |
2 |
2 |
0 |
||
Conjunctiva - Chemosis |
2 |
1 |
2 |
1 |
1 |
0 |
||
3 |
Cornea |
0 |
1 |
2 |
1 |
2A |
2AB |
0 |
Iris |
0 |
0 |
0 |
1 |
1 |
1 |
0 |
|
Conjunctiva - Erythema |
1 |
1 |
1 |
2 |
2 |
2 |
0 |
|
Conjunctiva - Chemosis |
2 |
2 |
2 |
2 |
1 |
1 |
0 |
A - Loss of Corneal Epithelium
B - Vascularization
C - Pannus
D - Dulling of the Cornea
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irreversible damage)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
No experimental data on irritation potential of the target substance is available. A read-across evaluation was developed to fill data gaps with LABS Na as the source substance.
Skin irritation
In the key, in vivo skin irritation study performed according to OECD guideline 404, three rabbits were exposed to 0.5 ml of the test substance dermally for 4 hrs. The test substance was covered with an occlusive dressing and removed by washing 4 hrs later. Animals were examined at 30 minutes after removal, and daily thereafter for 14 days. Irritation was noted in all animals at the first observation (maximum score of 2). Symptoms worsened, and desquamation, necrosis, and hyperkeratinization was noted by day 4. Symptoms resolved in one animal by day 12, but in the other two animals, symptoms were seen through the end of the observation period. The primary dermal irritation index was 2.17.
No in vitro skin irritation study is required as adequate in vivo data was already available.
Eye irritation
No experimental data on irritation potential of the target substance is available. A read-across evaluation was developed to fill data gaps with LABS Na as the source substance. The study examined the potential of the test substance to cause irritation to eyes. Groups of three rabbits had 0.1 ml of test substance placed in each of their eyes. In one group, the eyes were not rinsed. In the second group, the eyes were rinsed after 4 seconds of exposure. In the third group, the eyes were rinsed after 30 seconds of exposure. Observations were made one hour and 1, 2, 3, 4, 7, 14, and 21 days after exposure. Severe irritation was noted in the animals whose eyes were not rinsed. This irritation was not resolved in one of these animals at the end of 21 days. Irritation was also seen in the animals rinsed after 30 seconds, although the irritation was not as severe, and the effects were fully reversible within 14 days. Mild irritation was seen in the animals rinsed after 4 seconds. These effects were fully reversible within 7 days. Since OECD guideline 405 for eye irritation studies calls for an exposure of at least 24 hrs, the results for unrinsed eyes were used for classification. According to these results, the test substance is a category 1 eye irritant because it caused damage that was not reversible within 21 days.
Justification for classification or non-classification
The test substance is considered classified as category 2 (skin irritant) and category 1 (serious eye damage) according to CLP Regulation.
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