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EC number: 481-740-5 | CAS number: 848301-67-7
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
- key information: (96h) LL50 for freshwater fish (Pimephales promelas): >1000 mg/l [OECD 203; test mat. (WAFs) (nominal) based on: mortality];
- supporting information: (96h) LL50 for freshwater fish (Brachydanio rerio): >1000 mg/l [OECD 203; test mat. (WAFs) (nominal) based on: mortality].
Key value for chemical safety assessment
Additional information
The acute toxicity of a sample of GTL Gasoil (Shell SMDS-2) was determined by Palmer (2002) in a test with the fishPimephales promelas(Fathead minnow). The test was conducted in accordance with OECD Test Guideline 203.
Water-accommodated fractions (WAFs) of the sample were prepared in sealed vessels with minimum headspace, by stirring for approximately 72 hours. The contents of the vessels were left to stand for 1-2 hours before drawing off the aqueous phase – the WAF – for testing. Semi-static exposures were then carried out in completely full, sealed vessels. The tests were not subject to GLP and the test media were not analysed for stability or for exposure concentration of the test substance. However in other respects they were considered to fulfil the requirements of current best practice.
The test results, expressed as the LL50value, showed that the sample was not acutely toxic to fish at a loading rate of 1000 mg/l.
Albertus and Phillips (2005) have reported results for anacute toxicity test carried out ona second sample of the substance (SASOL SPD™ Diesel)with the fish Brachydanio rerio (Zebrafish).The test was conducted in accordance with OECD Test Guideline 201. The test media were WAFs of the test sample that were prepared in sealed vessels with a small headspace according to the CONCAWE methodology for the ecotoxicological testing of petroleum products (CONCAWE report, 1993).
The test results, expressed as the LL50value, showed that the sample was not acutely toxic to fish at a loading rate of 1000 mg/l.
The following information is taken into account for short-term toxicity to freshwater fish:
(96-h) LL50>1000 mg/l (P. promelas)
Value used for CSA:
96-h LL50for freshwater fish:>1000 mg/l.
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