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EC number: 447-010-5 | CAS number: 670241-72-2 ISONONYLBENZOAT
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
NOEC = 42.8 µg/L, based on analytical results; NOEC = 125 µg/L, based on nominal concentrations
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 42.8 µg/L
Additional information
The effects of the test item Isononyl benzoate to the early-life stage of fish (Zebrafish Danio rerio) were determined according to OECD guideline 210. A test was conducted under flow-through conditions with the nominal concentrations 62.5 - 125 - 250 - 500 - 1000 µg/L, corresponding to mean measured concentrations of 19.3 – 42.8 – 90.5 – 297 – 538 µg/L. The test was started by placing fertilised eggs in the test vessels and lasted 33 days (28 days post-hatch). 60 eggs of Danio reriowere exposed to the test concentrations and the control (4 replicates with 15 eggs each). Water quality parameters pH-value, oxygen concentration, temperature, hardness were determined to be within the acceptable limits. On day five 95 % of the control larvae had hatched. Therefore, study day 5 had been defined as post hatch day 0 (= PHD 0). Different toxic endpoints have been determined: egg hatch, time to hatch, fry growth (expressed as length and weight), morphological and behavioural effects, post hatch survival and fry survival. Chemical analysis (HPLC) of the test solutions was carried out in at least weekly intervals. Due to the test item properties adsorption and biodegradation were expected to occur. Therefore a flow rate equivalent to twenty test chamber volumes per 24 hours (guideline recommendation at least 5) had been set up in order to minimize absorbance and biodegradation effects. However, the mean recovery rates were in the range of 31 - 59 % of the nominal concentrations. For the lowest concentration most of the measurements were below the LOQ (0.03 mg Isononyl benzoate/L). With regard to the test item properties the analytical results were considered not to invalidate the test. All effect levels will be stated on base of both nominal and mean measured concentrations.
Findings and Observations
Based on the observation and statistical analysis of egg hatch, time to hatch, growth (expressed as weight and length), morphological and behavioural effects, post hatch survival and fry survival, the test revealed the following results:
Table1: Hatch, Growth, Morphological Effects, Fry survival: NOEC, LOEC
Based on nominal Isononyl benzoate concentrations and mean measured concentrations in brackets, respectively.
Hatch: |
Hatch on study days 3 to 5 resulted in a NOEC of ³1000 (538) µg/L and a LOEC of > 1000 (538) µg/L. |
Growth1) |
Length on PHD 28 resulted in a NOEC of ³ 250 (³ 90.5) µg/L and a LOEC of > 250 (> 90.5) µg/L1). |
Dry weight on PHD 28 resulted in a NOEC of ³ 250 (³ 90.5) µg/L and a LOEC of > 250 (> 90.5) µg/L.1) |
|
Morphological and behavioural effects |
Morphological and behavioural effects resulted in a NOEC of³ 250(³ 90.5) µg/L and a LOEC > 250 (> 90.5) µg/L. |
Post hatch survival |
Post hatch survival on PHD 28 resulted in a NOEC of 250 (90.5) µg/L and a LOEC 500 (90.5) µg/L. |
Overall survival |
Overall survival on PHD 28 resulted in a NOEC of 125 (42.8) µg/L and a LOEC of 250 (90.5) µg/L. |
Overall NOEC and LOEC |
Based on the parameters hatch, growth (expressed as length and dry weight), post hatch and overall survival the overall NOEC (post hatch day 0 – 28) was 125 µg/L (42.8µg/L) and the overall LOEC was 250 µg/L (90.5 µg/L). |
1) The concentrations 500 µg/L and 1000 µg/L were not considered to statistical evaluation due high mortality and reduced fish density in these replicates (number of surviving fish 80 % of the control).
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