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EC number: 278-636-5 | CAS number: 77182-82-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Ammonium 2-amino-4-(hydroxymethylphosphinyl)butyrate
- EC Number:
- 278-636-5
- EC Name:
- Ammonium 2-amino-4-(hydroxymethylphosphinyl)butyrate
- Cas Number:
- 77182-82-2
- Molecular formula:
- C5H12NO4P.H3N
- IUPAC Name:
- ammonium 2-amino-4-[hydroxy(methyl)phosphoryl]butanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- 2000 mg test item (content of: glufosinate-ammonium 50.8 %
w/w) were filled up to 200 mL with deionized water to achieve
a concentration of nominal 5 g/L of the active ingredient glufosinate-
ammonium.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Species: activated sludge, micro organisms from a domestic waste water
treatment plant.
Origin: supplied by the sewage plant Grofi-Zimmern, Germany
Conditioning:
The sludge was washed by centrifugation and the supernatant
liquid phase was decanted. The solid material was resuspended
in tap water and again centrifuged. The latter procedure was
repeated twice. An aliquot of the final sludge suspension was
weighed, dried and the ratio of wet sludge (g) to its dry weight
(g) determined. Based on this ratio, calculated aliquots of
washed sludge suspension, corresponding to 3 g dry material
per litre, were made up with tap water. The pH of the activated
sludge was determined to be pH 7.8. The activated sludge was
used directly for the test without storage.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- tab water
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18 - 19 °C
- pH:
- 7.8 (sludge)
7.3 - 8.3 (test) - Dissolved oxygen:
- 6.9 - 9.1 mg/L O2 / L
- Nominal and measured concentrations:
- 10, 32, 100, 320, 1000 mg/L nominal
- Details on test conditions:
- Surrounding Type:
controlled environment room
Aeration:
with compressed air (approximately 0.6 litre per minute)
Stock Solution of 3,5-Dichlorophenol:
2000 mg test item (content of: glufosinate-ammonium 50.8 % w/w) were filled up to 200 mL with deionized water to achieve a concentration of nominal 5 g/L of the active ingredient glufosinate- ammonium. 3,5-Dichlorophenol was used as a reference item (positive control): 0.5 g of 3,5-Dichlorophenol was dissolved according to the OECD Guideline No. 209 in 10 mL 1 mol/L NaOH and diluted to about 30 mL with deionized water. Excess of NaOH was neutralized with 0.5 mol/L H2SO4 to the point of incipient precipitation. Thereafter, the mixture was made up to one litre with deionized water. The final pH was measured to be 7.8 and the final concentration amounted to 500 mg/L.
Preparation of Test Flasks:
One test solution with a final volume of 500 mL was tested per treatment in a glass flask (>500 mL). Samples were prepared in about 15-minute intervals.
Measurement of Respiration Rate:
For the measurement of the respiration rate a well-mixed sample of each test medium was poured into a Karlsruher flask after exactly 3 hours incubation time and was not further aerated. The oxygen concentration was measured with an oxygen electrode and was recorded for about ten minutes. During measurement, the samples were continuously stirred on a magnetic stirrer. The oxygen consumption (in mg O2 / L minute) was determined from the most linear part of the respiration curve.
Measurement of pH, Dissolved Oxygen and water temperature:
The pH and the oxygen concentrations were determined at the start and at the end of the incubation period in all treatments. The water temperature was measured in one control medium at the start and the end of the incubation period. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Based on measured inhibition rates, the 3-hour EC 20 was determined to be 359 mg glufosinate-ammonium/L with 95 % confidence limits from 210 to 731 mg/L. The EC 50 could not be quantified because up to the highest nominal test concentration of 1000 mg glufosinate-ammonium/L less than 50 % inhibition was noted after three hours incubation. Nevertheless, the 3-hour EC 50 is clearly higher than 1000 mg glufosinate-ammonium/L under the present test conditions.
- Results with reference substance (positive control):
- The 3-hour EC 50 for the positive control 3,5-Dichlorophenol, which was tested in the same way as the test item, was found to be 5.4 mg/L and is within the range of 5 - 30 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC 50 could not be quantified because up to the highest nominal test concentration of 1000 mg glufosinate-ammonium/L less than 50 % inhibition was noted after three hours incubation. Nevertheless, the 3-hour EC 50 is clearly higher than 1000 mg glufosinate-ammonium/L under the present test conditions.
- Executive summary:
3h-EC50 > 1000 mg/L
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