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EC number: 268-083-8 | CAS number: 68002-70-0 This substance is identified by SDA Substance Name: C16-C22 trialkyl glyceride and SDA Reporting Number: 21-001-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- From Nov. 04, 2009 to Dec. 02, 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
Test material
- Reference substance name:
- Glycerides, C16-22
- EC Number:
- 268-083-8
- EC Name:
- Glycerides, C16-22
- Cas Number:
- 68002-70-0
- Molecular formula:
- Triglyceride containing a glycerol backbone esterified to fatty acids with a chain length varying from C16 to C22
- IUPAC Name:
- Tri (C16-22 fatty acyl) glycerol
- Details on test material:
- - Name of test material (as cited in study report): Fully hydrogenated high erucic acid rapeseed oil (CAS N° 84681-71-0, EC N° 283-532-8); under the SDA nomenclature, the name of this substance is ‘Glycerides, C16-22’
- Physical state: Solid (white yellow)
- Expiration date of the lot/batch: December, 2009
- Storage condition of test material: In original container, at room temperature (10-30 °C), in the dark
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant (Rossdorf, Germany)
- Preparation of inoculum for exposure: The activated sludge was washed by repeated centrifugation and re-suspension in tap water. The sediment of the last washing re-suspended in test medium and aerated until use.
- Pretreatment: The washed activated sludge was pre-conditioned in test medium for a maximum of 7 d
- Concentration of sludge: 1.5 g dry material per litre of test medium
- - Duration of test (contact time):
- ca. 28 d
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 85 mg KH2PO4, 217.5 mg K2HPO4, 334 mg Na2HPO4 x 2 H2O, 5 mg NH4Cl, 22.5 mg MgSO4 x 7 H2O, 36.4 mg CaCl2 x 2 H2O, FeCl3 x 6H2O and deionised water up to 1000 mL volume
- Test temperature: 22 ± 1 °C
- pH: 7.4 ± 0.2 (pH-electrode WTW pH 340i)
- Continuous darkness: Yes
- Other: Continuous stirring of test flasks in climatic chamber
TEST SYSTEM
- Culturing apparatus: Manometric test system with test flasks containing a volume of approximately 500 mL
- Number of culture flasks/concentration: Duplicate
- Measuring equipment: Pressure decrease (to determine oxygen consumption) measured using BSB Sensomat system, Aqualytic Dortmund
- Test performed in closed vessels: Yes (closed gas-tight by a measuring head)
- Details of trap for CO2 if used: Potassium hydroxide solution (45%) was used for trapping the produced carbone dioxide
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes (duplicate)
- Abiotic sterile control: Yes (single)
- Toxicity control: Yes (single)
- Other: Procedure control (single)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- ThOD: 1.666 mg oxygen per mg
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 29
- Sampling time:
- 1 d
- Remarks on result:
- other: None
- Details on results:
- - The 10 day window failed.
- Since the biodegradation did not reach the pass criterion of 60% degradation and no 10 day-window could be determined, the validity criterion was not applicable. At the end of the experiment, the difference in replicate variation was 0%.
- The test item contains little amounts of nitrogen, therefore the evaluation of biodegradation has to be based on the assumption that nitrification occurred, in the following expressed as ThODNO3.
However, the nitrification was not determined by means of an analytical method because the biodegradation rate was not higher than 60% based on ThODNH4
If no nitrification is considered, the mean biodegradation was also 65% after 28 days of incubation (ThODNH4), still failing the 10-d window.
BOD5 / COD results
- Results with reference substance:
- The reference item, sodium benzoate was degraded to more than 60% after 3 days of incubation. It was degraded to 90% after 14d and to 98% after 28d of incubation.
Any other information on results incl. tables
Inoculum control: The oxygen demand of the inoculum control (medium and inoculum) was 25 mg O2/L and thus not greater than 60 mg O2/L within 28 d as required by the test guideline.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, fully hydrogenated high erucic acid rapeseed oil was determined to be not readily biodegradable.
- Executive summary:
A study was conducted to determine the ready biodegradability of fully hydrogenated high erucic acid rapeseed oil according to OECD Guideline 301F and EU Method C4 -D.
Fully hydrogenated high erucic acid rapeseed oil was exposed to aerobic activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
Considering that fully hydrogenated high erucic acid rapeseed oil contains little amount of nitrogen, the nitrate concentration was measured. However, the nitrification was not determined by means of an analytical method because the biodegradation rate was not higher than 60% based on ThODNH4.
The reference item was degraded to more than 60% after 3 d of incubation.
Under the test conditions, fully hydrogenated high erucic acid rapeseed oil was determined to be not readily biodegradable.
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