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EC number: 254-074-6 | CAS number: 38668-46-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Dec - 27 Jan 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 Apr 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Württemberg, Germany (Karlsruhe, 10 Dec 2015)
Test material
- Reference substance name:
- 6-[2-(2-methyl-1H-imidazol-1-yl)ethyl]-1,3,5-triazine-2,4-diamine
- EC Number:
- 254-074-6
- EC Name:
- 6-[2-(2-methyl-1H-imidazol-1-yl)ethyl]-1,3,5-triazine-2,4-diamine
- Cas Number:
- 38668-46-1
- Molecular formula:
- C9H13N7
- IUPAC Name:
- 6-[2-(2-methyl-1H-imidazol-1-yl)ethyl]-1,3,5-triazine-2,4-diamine
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC-MS/MS
- Details on sampling:
- - Concentrations: All test item concentrations and the control at test start and after 24 h from fresh and aged solutions and after 48 h from aged solutions.
- Sampling method: Samples were taken from fresh media at 0 h and 24 h and from aged media at 24 and 48 h.
- Sample storage conditions before analysis: All samples were deep frozen (≤ -18 °C) until they were transferred to the analytical laboratory.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 100 mg/L stock solution was prepared by homogenising an appropriate amount of test item with an appropriate amount of test medium by treatment with 10 min ultrasonication. Afterwards, the solution was clear and transparent. Lower test solutions were prepared by dilution of the stock solution with test medium.
- Differential loading: No
- Controls: Untreated test medium
- Evidence of undissolved material: The stock solution was clear and transparent.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Clone V
- Age of daphnids at test start: Freshly hatched daphnids, less than 24 h old
- Source: In-house culture of testing facility, originally purchased from the Federal Environment Agency in Berlin, Germany.
- Culture conditions: Single culture (1 daphnid per 100 mL) in aerated Elendt M4 medium, at 20 ± 2 °C and pH 6.0 - 9.0. The dissolved oxygen was > 60% saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14 °dH. The daphnids were fed with unicellular green algae (Desmodesumus subspicatus) at least 3x/week. The medium was changed 3x/week.
- Feeding during test: No feeding during test
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 214 mg/L (CaCO3) = 12 °dH (in test medium)
- Test temperature:
- 18.8 - 20.4 °C
- pH:
- 7.60 - 8.44
- Dissolved oxygen:
- ≥ 8.4 mg/L
- Nominal and measured concentrations:
- Control, 0.01, 0.10, 1.00, 10.0, and 100.0 mg/L (nominal)
Control, -- , --, --, --, and 100 - 114 mg/L (measured) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers filled with 50 mL test solution and covered with a glass plate
- Aeration: None
- Renewal rate of test solution: After 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 daphnid/10 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium according to guideline
- Culture medium different from test medium: Culture medium same as test medium
- Intervals of water quality measurement: Test temperature, pH and oxygen concentrations were measured in one separate replicate of each test item level (without daphnids) in fresh test solutions at t = 0 and 24 h and in aged solutions at t = 24 and 48 h
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/ 8 h dark
EFFECT PARAMETERS MEASURED:
- Immobilization: After 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: A semi-static GLP range-finder test was performed in a limit test design with nominal test solution concentrations of 0.01, 0.10, 1.00, 10.0, and 100.0 mg/L and a control.
- Results used to determine the conditions for the definitive study: Yes, no immobilisation was observed after 48 h in the control and at test item concentrations from 0.10 to 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate, tested periodically
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No behavioural changes were observed.
- Other biological observations: After 24 h no immobilization was observed in the control and up to and including the 100 mg/L treatment level. After 48 h no immobilization occurred in the control and at test item concentrations of 0.10 - 100 mg/L. At 0.01 mg/L no immobilization higher than the permitted control immobilization was observed.
- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- EC50 (24 h): 1.00 mg/L (potassium dichromate)
- Other: Reference test dating 12 Dec 2017 - Reported statistics and error estimates:
- Due to lack of immobilization, no statistical analysis was performed.
Any other information on results incl. tables
VALIDITY CRITERIA
The study fulfilled the validity criteria laid down by the guideline (Table 1).
Table 1. Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
In this study the control immobilisation was 0 %. |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
In this test, the dissolved oxygen concentration at the end of the test was≥8.4 mg/L. |
Yes |
ANALYTICAL RESULTS
The measured test item concentrations in fresh samples (0 h) ranged between 100 – 109% of nominal concentrations, with a mean initial concentration of 105% of nominal. In aged samples (24 h) the measured test item concentrations ranged from 107 to 114% of nominal, with a mean measured concentration of 111% of nominal (Table 2). Therefore, the effect values were based on nominal test item concentrations.
Table 2. Measured test item concentrations.
nominal test item concentration |
sampling time
|
measured concentration |
|
[mg/L] |
[h] |
[mg/L] |
% of nominal |
100 |
0 h, fresh |
100 |
100 |
24 h, fresh |
109 |
109 |
|
|
|
|
|
24 h aged |
107 |
107 |
|
48 h, aged |
114 |
114 |
BIOLOGICAL RESULTS
After 24 h, no immobilization was observed in all test levels and the control.
After 48 h, no significant immobilization was observed in all test levels and the control. At the 0.01 mg/L treatment level, 5% immobilization (1/20 daphnids) was observed, which is below the permitted control immobilization of 10%.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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