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EC number: 231-324-2 | CAS number: 7492-67-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From July 12, 2019 to October 29, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- [(3,7-dimethyl-6-octenyl)oxy]acetaldehyde
- EC Number:
- 231-324-2
- EC Name:
- [(3,7-dimethyl-6-octenyl)oxy]acetaldehyde
- Cas Number:
- 7492-67-3
- Molecular formula:
- C12H22O2
- IUPAC Name:
- 2-[(3,7-dimethyloct-6-en-1-yl)oxy]acetaldehyde
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Batch: VE00601569
Appearance: Colorless to pale yellow liquid
Expiration Date: January 12, 2020
Purity: 92.9% (sum of the two peaks)
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- GC-FID
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain No. 61.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, 37073 Göttingen / Germany).
Study design
- Test type:
- static
- Water media type:
- other: The algae were cultivated and tested in synthetic test water (AAP Medium), prepared according to the test guidelines, but modified according to the International Standard ISO 14442 [2] as a closed test system was applied.
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- The test flasks were incubated in a temperature controlled orbital shaker (Multitron-Pro, Infors HT, Bottmingen/Switzerland) at a temperature of 21°C.
- pH:
- The pH was measured and recorded in each treatment at the start and end of the test.
- Nominal and measured concentrations:
- Dilutions tested: 1:2.2, 1:4.6, 1:10 and 1:22. Additionally, a control (test water without test item) was tested in parallel.
- Details on test conditions:
- The test flasks were positioned randomly and repositioned daily. They were continuously illuminated by LED light installed above the test flasks. The light intensity at the level of the test solutions was approximately 75 µE s-1 m-2 (range: 70 to 79 µE s-1 m-2 , measured at nine places in the experimental area).
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Remarks:
- 95% Confidence Interval
- Effect conc.:
- 15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Remarks:
- 95% Confidence Interval
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- 95% Confidence Interval
- Effect conc.:
- 22 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.4 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 9.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- For evaluation of the algal quality and experimental conditions, potassium dichromate is tested as a positive control twice a year to demonstrate satisfactory test conditions. The 72-hour EC50 for growth rate in the reference test IES Study Number 20190094 was 1.1 mg/L (May 2019) and showed that the sensitivity of the test system was within the range recommended by the guideline (72-hour EC50 for the growth rate 0.9-1.5 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A clear concentration-response relationship was observed for both biological endpoints growth rate and yield after the exposure period of 72 hours. A statistically significant inhibitory effect on the growth rate and yield of the algae was observed at the end of the test at the mean measured concentration of 9.2 mg/L and at all higher test concentrations.
- Executive summary:
The impact of the test item CITRONELLYL OXYACETALDEHYDE on the growth of the freshwater green algal species Pseudokirchneriella subcapitata was investigated in a 72-hour static test according to the OECD Guideline 201 (2006, corrected 2011) and the Commission Regulation (EU) No 2016/266, C.3.
As the test item is a volatile substance, the test was performed using glass Erlenmeyer completely filled (without headspace) with test medium that were tightly sealed with glass stoppers to avoid losses of test item by evaporation (closed system).
As the test item is a liquid with low water solubility, the slow-stirring method (to avoid formation of micro-droplets) was applied for preparation of a saturated test item solution. For preparation of the highest concentration of test medium, the test item was carefully applied (pipetted) onto the surface of the test water at a loading rate of 100 mg/L. Thereafter slow-stirring was applied for 24 hours in a closed vessel.
After this treatment the lower part of the equilibrated test medium was carefully harvested from the stirring vessel through a tap at the bottom of the vessel. This equilibrated aqueous phase with a loading rate of 100 mg/L, containing dissolved test item only, was used as the highest concentration and was diluted with test water to obtain the dilutions 1:2.2, 1:4.6, 1:10 and 1:22. Additionally, a control (test water without test item) was tested in parallel.
The preparation of the test media was based on the OECD Guidance Document No. 23 on Aqueous-Phase Aquatic Toxicity Testing of Difficult Test Chemicals, 2019.
The daily measured concentrations of the test item CITRONELLYL OXYACETALDEHYDE in the test media are shown in the table below. The analytical measurements from test media at the start of the test shown the correct preparation of the test media, i.e. the theoretical spacing factor of 2.2 between the different test concentrations was met. Since the test item concentrations decreased over the test period, the mean measured concentrations were calculated as the time-weighted geometric mean of the concentrations measured at all sampling dates (0, 24, 48, and 72 hours).
Treatment / Dilution# Analytically Measured Concentration of the Test Item [mg/L] Mean Measured Concentration (Geometric Mean) [mg/L] 0 Hours 24 Hours 48 Hours 72 Hours 1:22 2.52 2.36 1.43 0.624 1.6 1:10 5.03 5.03 4.33 3.06 4.4 1:4.6 10.1 9.85 8.88 8.08 9.2 1:2.2 20.7 19.9 17.6 18.1 19 Undiluted Test Medium* 40.4 41.7 43.7 39.6 42 *: Equilibrated test medium with a loading rate of 100 mg/L.
#: Dilutions of the equilibrated test medium with a loading rate of 100 mg/L.
The biological results are based on the mean measured test item concentrations.
A clear concentration-response relationship was observed for both biological endpoints growth rate and yield after the exposure period of 72 hours. A statistically significant inhibitory effect on the growth rate and yield of the algae was observed at the end of the test at the mean measured concentration of 9.2 mg/L and at all higher test concentrations.
Endpoint after 72 Hours Growth Rate [mg/L] Yield [mg/L] EC10
(95% Confidence Interval)15
(14-16)5.9
(5.6-6.3)EC20
(95% Confidence Interval)17
(16-18)7.6
(7.3-8.0)EC50
(95% Confidence Interval)22
(21-23)12
(12.0-12.7)NOEC 4.4 4.4 LOEC 9.2 9.2 Validity Criteria: The validity criteria for increase of biomass, mean coefficient of variation of the daily growth rates and coefficient of variation of the average specific growth rates were fulfilled for the control.
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