N,N-diethyl-2-propynylamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Version / remarks:

September, 1984

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (as cited in study report): Golpanol DP

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Duration of test (contact time):

28 d

Initial conc.:

>= 10 - <= 40 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

The test batches contain inorganic salts dissolved in distilled water and the test substance in a concentration of about 10 to max. 40 mg/L based on their carbon content. At certain intervals, a sample is taken and the dissolved organically bound carbon (= DOC) is determined. The experiment was performed in duplicate.

Reference substance:

not specified

Key result

Parameter:

% degradation (DOC removal)

Value:

73

Sampling time:

28 d

Remarks on result:

other: calculated from 2 experiments

Details on results:

Biodegradation of the test substance was observed: After 7 days, the amount of DOC was decreased by 53% and 41% in replicate 1 and 2, respectively. After 28 days, DOC-content was decreased by 81% and 65% for replicate 1 and 2, respectively. After 28 days, the test substance was degraded for 73% (mean value). See 'Any other information on results incl. tables'.

Measured DOC and inhibition throughout the test

Time	Test substance replicate 1	Decrease (% degradation)	Test substance replicate 2	Decrease (% degradation)
Study initiation	DOC = 21.6 mg/L	---	DOC = 21.6 mg/L	---
7 Days	DOC = 10.3 mg/L	53%	DOC = 12.8 mg/L	41%
14 Days	DOC = 6.6 mg/L	70%	DOC = 9.9 mg/L	54%
21 Days	DOC = 4.7 mg/L	78%	DOC = 7.7 mg/L	64%
25 days	DOC = 4.7 mg/L	78%	DOC = 7.7 mg/L	64%
28 Days	DOC = 4.2 mg/L	81%	DOC = 7.6 mg/L	65%

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable, but failing 10-day window

Description of key information

The substance is readily biodegradable but failing 10-day window as observed in a Modified OECD Screening test according to OECD TG 301E.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable but failing 10-day window

Additional information

The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301E (Modified OECD Screening (mod. 301A)) (GLP not specified) (BASF, 1987). In this study, 10 to 40 mg/L test substance (expressed as DOC) was inoculated with non-adapted activated sludge from a predominantly domestic sewage treatment plant for 28 days under aerobic conditions. The DOC-content was determined after 7, 14, 21, 25 and 28 days incubation. The observed decrease in measured DOC-content was expressed as % degradation. After 7 days, the DOC-content was decreased by 53% and 41% in replicate 1 and 2, respectively. After 28 days, the DOC-content had decreased by 81% and 65%, respectively. The mean biodegradation after 28 days was calculated to be 73%. Based on the results, the substance is classified as readily biodegradable. The adaptation phase of the inoculum cannot be accurately determined due to the limited amount of determinations of DOC-content. Consequently, it cannot be determined if the 10-day window criterion was met.