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EC number: 202-803-3 | CAS number: 99-94-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Remarks:
- Intraperitoneal injection
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The test substance was injected intraperitoneally in a volume of 10 ml/kg bw .
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- p-toluic acid
- EC Number:
- 202-803-3
- EC Name:
- p-toluic acid
- Cas Number:
- 99-94-5
- Molecular formula:
- C8H8O2
- IUPAC Name:
- 4-methylbenzoic acid
- Test material form:
- solid: crystalline
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Lot no. 447334
- Expiration date of the lot/batch:
- Purity test date:
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
- Stability under test conditions:
- Solubility and stability of the test substance in the solvent/vehicle:
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:
FORM AS APPLIED IN THE TEST (if different from that of starting material)
OTHER SPECIFICS:
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Chemicals Evaluation and Research Institute (CERI) Tokyo
- Age at study initiation:
- Weight at study initiation:
- Fasting period before study:
- Housing: Individually
- Diet (e.g. ad libitum): During experiments, animals were allowed to access food and water ad libitum.
- Water (e.g. ad libitum): During experiments, animals were allowed to access food and water ad libitum.
- Acclimation period:
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23 +/- 1°C
- Humidity (%): 55 +/-5%
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light):
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- other: Gum arabic saline
- Details on exposure:
- The test substance was injected intraperitoneally. Ten male rats were assigned to doses of 0 (vehicle control), 751, 826, 909, 1000, and 1100 mg/kg. Ten female rats were assigned to doses of 0 (vehicle control), 683, 751, 826, 909, 1000, and 1100 mg/kg.
- Doses:
- Ten male rats were assigned to doses of 0 (vehicle control), 751, 826, 909, 1000, and 1100 mg/kg.
Ten female rats were assigned to doses of 0 (vehicle control), 683, 751, 826, 909, 1000, and 1100 mg/kg. - No. of animals per sex per dose:
- 10
- Control animals:
- not specified
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 944 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 859 - <= 1 037
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 874 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 790 - <= 967
- Mortality:
- Acute toxicity of p-toluic acid in rats (i.p.)
==================================
Dose Mortality
Sex (mg/kg) (Dead / tested)
----------------------------------
751 0/10
826 1/10
909 3/10
male 1000 7/10
1100 10/10
----------------------------------
683 0/10
751 1/10
826 2/10
female 909 7/10
1000 9/10
1100 10/10 - Clinical signs:
- No clinical signs were observed in the control animals.
In the treated animals, clinical signs, such as sedation, a decrease in spontaneous locomotion, prone position, limb weakness, abasia, ptosis, lacrimation, nasal hemorrhage, coma, loss of righting reflex, loss of hearing, touch and pain reflex, loss of pinna and corneal reflex, a decrease heart rate, subnormal temperature, deep respiration, bradypnea, respiratory arrest and death, were observed shortly after the injection of p-toluic acid. In the surviving animals, all clinical signs disappeared by Day 5. - Body weight:
- Dose-depended suppression of the body weight gain was observed in the females at 751-909 mg/kg bw. The body weight of the other treated animals was approximately equal to that of the control animals.
- Gross pathology:
- In half of the animals, nasal hemorrhage and reddish tear were observed. In the animals that died within 4 hours after injection, some amount of the test substance still remained at an injection site. In most animals that died within 24 hours after injection, ascites, petechial hemorrhages in the mucosa of small intestine, congestion of the lung, liver and mucosa of small intestine were observed. In the animals that died on the second day after injection and in surviving ones, slight to mild thickening of the quadrate lobe of liver, dull margin of the quadrate lobe and left hepatic lobe, the adhesion between the quadrate and right lobe of the liver and the adhesion between love of liver and the peritoneum were observed
Applicant's summary and conclusion
- Conclusions:
- Although this intraperitoneal injection study in the rat returned results of LD50 male 944 mg/kg bw and female 874 mg/kg bw, which implies a classification of Acute Toxicity 4, it will not be used for classifcation as this is not an accepted route for human exposure.
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