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EC number: 220-599-4 | CAS number: 2832-30-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: 16 CFR 1500.41
- GLP compliance:
- no
- Remarks:
- Study conducted prior to implementation of GLP
Test material
- Reference substance name:
- 1,4-dichloro-5,8-dihydroxyanthraquinone
- EC Number:
- 220-599-4
- EC Name:
- 1,4-dichloro-5,8-dihydroxyanthraquinone
- Cas Number:
- 2832-30-6
- Molecular formula:
- C14H6Cl2O4
- IUPAC Name:
- 1,4-dichloro-5,8-dihydroxy-9,10-dihydroanthracene-9,10-dione
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Lippische Versuchstierzucht / Extertal 1
- Weight at study initiation: 2.1 kg (average)
- Housing:individual cages
- Diet (e.g. ad libitum): "mümmel z" (ssniff / Soest)
- Water (e.g. ad libitum): yes
- Acclimation period: 7-14 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 16-18
- Humidity (%): 40 r.h.
- Photoperiod (hrs dark / hrs light): 12 / 12
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- other: both sides of trunk clipped, one side with additional scarification
- Vehicle:
- other: oil
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: 500 mg/ gauze
VEHICLE
- oil - Duration of treatment / exposure:
- 24 hrs
- Observation period:
- 30 minutes after end of application followed by 48 and 72 hrs after start of application, observation control after 8 d
- Number of animals:
- 6
- Details on study design:
- TEST SITE
- Area of exposure: 2.5x2.5 cm on both sides of the trunk (one side intact, the other side scarified)
- Type of wrap if used: double layer of surgical gauze, covered with impermeable PVC foil, fixed with Leukoflex on both sides of the trunk, elastic bandage, animals were immobilized during exposure.
OBSERVATION TIME POINTS
30 minutes after end of application followed by 48 and 72 hours after start of application (observation results after 48 hours not stated in report), observation control after 8 days
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72
- Remarks:
- h
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72
- Remarks:
- h
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72
- Remarks:
- h
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #4
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #5
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #6
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72
- Remarks:
- h
- Remarks on result:
- other:
- Remarks:
- scarificed skin
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72
- Remarks:
- h
- Remarks on result:
- other:
- Remarks:
- scarificed skin
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarificed skin
- Irritation parameter:
- edema score
- Basis:
- animal #4
- Time point:
- 24 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72
- Remarks:
- h
- Remarks on result:
- other:
- Remarks:
- scarificed skin
- Irritation parameter:
- edema score
- Basis:
- animal #5
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarificed skin
- Irritation parameter:
- edema score
- Basis:
- animal #6
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarificed skin
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 48 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- not determined
- Remarks on result:
- other:
- Remarks:
- not determined
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #4
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #5
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #6
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- intact skin
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarified skin
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarified skin
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarified skin
- Irritation parameter:
- edema score
- Basis:
- animal #4
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarified skin
- Irritation parameter:
- edema score
- Basis:
- animal #5
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarified skin
- Irritation parameter:
- edema score
- Basis:
- animal #6
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: score = 0
- Remarks on result:
- other:
- Remarks:
- scarified skin
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 24 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring of skin by testing material.
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring of skin by testing material.
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 24 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 24 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #4
- Time point:
- 24 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #5
- Time point:
- 24 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #6
- Time point:
- 24 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 48 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- not determined
- Remarks on result:
- other:
- Remarks:
- not determined
- Irritation parameter:
- erythema score
- Basis:
- animal #1
- Time point:
- 72 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #2
- Time point:
- 72 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #3
- Time point:
- 72 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #4
- Time point:
- 72 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #5
- Time point:
- 72 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritation parameter:
- erythema score
- Basis:
- animal #6
- Time point:
- 72 h
- Score:
- >= 0
- Max. score:
- 4
- Reversibility:
- other:
- Remarks:
- scoring not possible due to colouring
- Remarks on result:
- other:
- Remarks:
- scoring not possible due to colouring
- Irritant / corrosive response data:
- Erythema: due to colouring of the skin by testing material no scoring could be conducted.
Edema: four animals (#1 to #4) showed very slight edemae on intact skin within 24 hours after application start, which were fully reversible within 48 hours. Slight and very slight edemae were observed within 24 hours after application start on scarified skin in animals #2 and #4, respectively, which were fully reversible within 48 hours. No effects were observed in any other animal during the observation period.
Any other information on results incl. tables
Irritant/corrosive response data for each animal at each observation time up to removal of each animal from the test (results from intact skin, only)
Score at time point / Reversibility |
Erythema |
Edema |
Max. score: 4 |
Max. score: 4 |
|
24 h |
** |
1/1/1/1/0/0 |
48 h (not measured) |
** |
-- |
72 h |
** |
0/0/0/0/0/0 |
Average 24h/72h |
** |
0.5/0.5/0.5/0.5/0/0 |
Reversibility*) |
** |
c/c/c/c/--/-- |
*) Reversibility: c. = completely reversible; n.c. = not completely reversible; n. = not reversible
**) scoring not possible due to colouring of skin by testing material
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- 5,8-Dichlorchinizarin was not irritating in the in vivo skin irritation test under the experimental conditions described in this report.
- Executive summary:
In a primary dermal irritation study performed in accordance with the Code of Federal Regulations, Title 16, Section 1500.41, adult albino rabbits (strain New Zealand White) were dermally exposed to 500 mg of 5,8-Dichlorchinizarin (vehicle: oil) under occlusive conditions for 24 hours to 2 x 6.25 cm² body surface area. Animals were then observed for 8 days.
Erythema was not scored due to colouring of skin by testing material. Edema scores of 1 were observed in 4 out of 6 animals (#1-#4) within 24 hours after application start in which animal #2 and #4 also showed effects on scarified skin with a score of 2 and 1, respectively. The 24h/72h average edema score was 0.5 in four animals and 0 in the other two animals. However, both irritation scores lead to classification as non-irritating.
In this study, 5,8-Dichlorchinizarin is not a dermal irritant.
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