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EC number: 230-991-7 | CAS number: 7397-62-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 23. Aug. 2007 - 24. Apr. 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 301B
- Version / remarks:
- The results of the test on ready biodegradability were used in a weight of evidence approach for the derivation of the PNEC STP
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Batch No.of test material: Probe vom 07.08.2007
- Expiration date of the batch: 09.08.2008
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored at room temperature in the dark.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: no - Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture:
- Name and location of sewage treatment plant where inoculum was collected: The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, 1m Altenschemel, NW-Lachen-Speyerdorf
- Pretreatment: The sludge was filtrated , washed with tap water twice, then washed with and re-suspended in test medium. It was then aerated until use.
- Initial biomass concentration: The dry matter was determined with 4860 mg suspended solids/litre. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Test temperature:
- 22 ± 2°'C
- pH:
- 7.4 +/- 0.1
- Nominal and measured concentrations:
- 37.1 mg/L (mean of two concentrations), corresponding to an organic carbon content of 20 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1500 ml glass flasks
- Type (delete if not applicable): open
Inoculum concentration:
Temperature:
Duration: 28 days
- No. of vessels per concentration (replicates): 2, containing test item, mineral medium and inoculum
- No. of vessels per control (replicates): 2, containing mineral medium and inoculum
- No. of vessels per toxicity control (replicates): 1, containing test item, reference item, mineral medium and inoculum
- No. of vessels per abiotic control (replicates): 1, containing test item, mineral medium and HgCI2
- Sludge concentration (weight of dry solids per volume): 24.9 mg suspended solids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Particulate matter:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:
- Details on termination of incubation:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using fewer concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- anilin
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 37.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: CO2 evolution
- Validity criteria fulfilled:
- yes
- Conclusions:
- Polysolvan O is readily biodegradable according to the definition of the OECD 301B. As no toxic effects of the test substance to the inoculum were observed during the exposure time of 28 days, the results of this test can be used for assessing the toxicity to microorganisms in a weight of evidence approach.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards, well documented and acceptable for assessment.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- Remarks:
- The exposure period was 18 hours, not 16 hours as mentioned in the guideline
- Principles of method if other than guideline:
- Toxicity to Pseudomonas putida was determined in two different ways: In the first approach, toxic effects were determined by means of the commonly used growth inhibition test according to DIN 38 412 as described in doc no T01697. In the second approach, toxic effects on Pseudomonas putida were determined by means of ATP measurement.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- as described in T01697
- Vehicle:
- not specified
- Details on test solutions:
- The nutrient media were prepared according to the method described in T01697, however, the pre-culture medium contained 50 mg/L yeast extract instead of trace element and vitamin solution.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- as described in T01697
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 18 h
- Nominal and measured concentrations:
- Nominal concentrations are given
- Details on test conditions:
- Type: aquatic
- Reference substance (positive control):
- not specified
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 454 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: standard test
- Duration:
- 18 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: standard test
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 347 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: ATP measurement
- Remarks on result:
- other: ATP test
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 240 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: ATP measurement
- Remarks on result:
- other: ATP test
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- no reference substance was tested
- Reported statistics and error estimates:
- Effect concentrations were determined graphically by means of a dose-response curve. For the standard growth inhibition test, a coefficient of variation of 0.5 - 1% is given for the parallel replicates.
- Validity criteria fulfilled:
- not applicable
- Executive summary:
The possible toxic effects of Polysolvan O to bacteria was tested with Pseudomonas putida in two different test approaches. In the first, the standard growth inhibition test according to DIN 38 412 part 8 was conducted were the effects were determined by photometrical determination of opacity. The second test was
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1 day
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study is very poorly described, but it provides valuable information and meets accepted scientific standards. This present report was newly generated in 2009 from archived laboratory manuscripts of the year 1986.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ETAD Fermentation Tube Method
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- anaerobic bacteria from a domestic water treatment plant
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Details on test conditions:
- Type: aquatic
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 2 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: reduction of gas production
- Validity criteria fulfilled:
- not specified
- Remarks:
- A validation of the results is not possible, as neither validation criteria were documented, nor the absolute values of the gas production activities were recorded.
- Conclusions:
- The EC0 of Polysolvan O in the fermentation tube test was 2500 mg/L.
Referenceopen allclose all
Description of key information
Pseudomonas putida: EC10 (18 hr): 454 mg/l
Pseudomonas putida: EC50 (18 hr): 2320 mg/l
ETAD fermentation tube test: EC0 (24 hr): 2500 mg/l
Ready biodegradability: 37.1
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 37.1 mg/L
Additional information
No respitration inhibition test is available for Polysolvan O. But three studies are available which can be used for the assessment of the toxicity to microorganisms in a weight of evidence approach.. In the so-called ETAD fermentation tube test, the toxicity to microorganisms under anaerobic conditions was assessed. The EC0 was determined to be 2500 mg/L after an exposure period of 24 hours. The second study was conducted under aerobic conditions using Pseudomonas putida as model organism. The reported EC10 in this test was 454 mg/l after an exposure period of 18 h. The third study is a test on ready biodegradability of Polysolvan O. No toxic effects of the substance could be shown at a test concentration of 37.1 mg/L (i.e 20 mg organic carbon/L) which is the lowest value of any of the available study on toxicity to microorganisms. This study is a guideline study (OECD 301B) and was performed according to GLP and was hence considered to be the most reliable one. According to chapter 10 of the IR&CSR, a study on ready biodegradability can be used for the derivation of the PNEC for STP if not study on toxicity to microorganisms is available. Therefore, the NOEC derived from this study can be used for the development of the PNEC for microorganisms.
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