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EC number: 234-014-5 | CAS number: 10489-46-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- Few deviations occurred (i.e. T°C and measurement period differed from the study plan). Both reported to be uncritical, normal respiration activity of the control was observed and correlation of the inhibition values within the test replicates was good.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Diammonium sodium hexakis(nitrito-N)rhodate
- EC Number:
- 264-713-0
- EC Name:
- Diammonium sodium hexakis(nitrito-N)rhodate
- Cas Number:
- 64164-17-6
- Molecular formula:
- H4N.1/2N6O12Rh.1/2Na
- IUPAC Name:
- Diammonium sodium hexakis(nitro-N)rhodate
- Reference substance name:
- Diammonium sodium hexakis (Nitrito-N) rhodate
- IUPAC Name:
- Diammonium sodium hexakis (Nitrito-N) rhodate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - Method: In the control vessels, 16 mL nutrient solution was mixed with both 234 and 231 mL dilution water (i.e. tap water), without and with the addition of the nitrification inhibitor N-allylthiourea (ATU), respectively. Both the positive control vessels and the treatments were prepared by putting the appropriate amount of positive control solution, test item nominal concentrations, and ATU into the respective test vessel (concentrations of the test item and positive control were calculated using the concentration of the respective stock solution and the dilution factor). Then, 16 mL nutrient solution and water to 250 mL were added. Lastly, 250 mL inoculum was added in 5 minute intervals and the mixtures were aerated.
After 3 hours, the content of the first vessel was poured in a 250 mL narrow-deck bottle and the respiration rate was determined by measurement of the oxygen concentration over a period of max 5 minutes. The following vessels were measured in five minute intervals.
- Differential loading: Test item nominal concentrations were 1000, 100, 10, 1 mg/L in the first experiment, and 1000 mg/L in the second one.
- Controls: Both blank and positive controls were used. Blank controls contained 16 mL nutrient solution, 250 mL inoculum, and either 234 mL or 231 mL, according to the absence or addition of ATU, respectively. 3,5-Dichlorophenol (CAS 591-35-5) was used as positive control. A stock solution in deionised water containing 500 mg/L (nominal) was freshly prepared for each experiment.
- Nutrient solution: Synthetic sewage that was frozen immediately after preparation.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from a predominantly domestic sewage treatment plant was collated on the day before treatment. Sludge was filtrated, washed in tap water, resuspended in tap water, aerated until usage in the test, and fed daily with 50 mL synthetic sewage feed/L. The dry matter was determined, and volume was adapted to the desired content of dry matter. The dry matter content of the inoculum was also determined on the day of each experiment, and the following results were obtained, respectively:
Exp. 1) The dry matter was determined as 3.06 g suspended solids/L, giving a concentration of 1.53 g suspended solids/L in the test;
Exp. 2) The dry matter was determined as 2.74 g suspended solids/L, giving a concentration of 1.37 g suspended solids/L in the test.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- -
- Test temperature:
- First experiment: range from 20.0 to 22.6 °C
Second experiment: range from 20.7 to 21.8 °C - pH:
- First experiment: pH range from 7.7 to 8.0
Second experiment: pH range from 8.1 to 8.3 - Dissolved oxygen:
- -
- Salinity:
- -
- Nominal and measured concentrations:
- Test item nominal concentrations of 1000, 100, 10, 1 mg/L were used in the first experiment, and the sole test item nominal concentration of 1000 mg/L was used in the second experiment as a limit test.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Aeration: Purified air, using Pasteur pipettes
- No. of vessels per concentration (replicates): 1 replicate per treatment (first experiment), and 5 replicates per treatment (second experiment).
- No. of vessels per control (replicates): 2 replicates before and at the end of the exposure period, both for the test item and positive control treatments.
- No. of vessels per positive control (replicates): 1 replicate per treatment.
- Biomass loading rate: 250 mL activated sewage inoculum
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water
TEST CONCENTRATIONS
- Spacing factor for test concentrations: A spacing factor of 10 was used
- Test concentrations: Nominal test concentrations of 1000, 100, 10, 1 mg/L were used for the first experiment. A nominal concentration of 1000 mg/L was used in the second experiment, as a limit test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (CAS 591-35-5)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: without ATU
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: without ATU
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: with ATU
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: with ATU
- Details on results:
- The oxygen consumption rate was calculated from the slope of the linear part of the oxygen consumption curve using linear regression.
- Results with reference substance (positive control):
- The calculated 3h-EC50 values for the positive control were:
First experiment without ATU - 12 mg/L (95% C.I. 9.2 - 16 mg/L), and with ATU - 12 mg/L (95% C.I. 8.4 - 16 mg/L)
Second experiment without ATU - 12 mg/L (95% C.I. 8.4 - 16 mg/L), and with ATU – 12 mg/L (95% C.I. 7.3 - 19 mg/L)
All values lay within the recommended range of 2 - 25 mg/L (total respiration without ATU), and 5 - 40 mg/L (heterotrophic respiration with ATU). - Reported statistics and error estimates:
- A statistical determination of the NOEC was carried out, in order to test whether the differences between the related effect level of 1000 mg/L and the control were significant. For this determination, the values of the oxygen consumption were used. Firstly, it was checked whether equality of variance was given, and afterwards a t-test was applied. No significant differences were found between the treatment of 1000 mg/L and the control.
A linear fit on a probability-logarithmic scale was used for evaluating EC50 values.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC value for both total respiration and heterotrophic respiration was determined to be ≥ 1000 mg/L, corresponding to >=146 mg Rh/L.
- Executive summary:
This study evaluated the effect of Diammonium sodium hexakis (Nitrito-N) rhodate on the respiration of activated sludge (Muckle 2015). The study is reliable without restrictions, being GLP compliant and having followed the standard guidelines OECD 209, and EU-Method C.11. The duration of the test was 3 hours. Activated sludge, which was collected from a predominantly domestic sewage treatment plant, was used as inoculum. The substance 3,5-dichlorophenol was used as a positive control. Two experiments were carried out, a range-finding test followed by a limit test. All validity criteria were met.
At the nominal concentration of 1000 mg/L, no significant inhibition was observed in treatments with and without the nitrification inhibitor N-allylthiourea (ATU). The respective NOEC and EC10 values for both total respiration and heterotrophic respiration was determined to be ≥ 1000 mg/L nominal concentration (corresponding to >=235 mg Rh/L), and the EC50 value was >1000 mg/L, corresponding to >=146 mg Rh/L..
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