Carbonic acid, C,C'-[4-(2-bromoacetyl)-1,3-phenylene] C,C'-dimethyl ester

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28.05. - 28.07.2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Details on sampling:

not applicable

Vehicle:

no

Details on test solutions:

Elendt M4 medium was used as test medium (composition in accordance with OECD 202). At test initiation the pH-value of the control (untreated test medium) was 8.33, the dissolved oxygen concentration was 8.9 mg/L and the total hardness was 14°dH (250 mg/L as CaCO3).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Strain: Daphnia magna Straus, Clone V
- Source: Federal Environment Agency (Umweltbundesamt in Berlin/Germany).
- Age at study initiation (mean and range, SD): Freshly hatched daphnids max. 24 hours old were used for the test.
- Method of breeding: Daphnia magna was bred as group culture (5 to 20 daphnids per litre) in Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, former Scenedesmus subspicatus) at least three times a week.
- Feeding during test: None
- Food type: single cell green algae (Desmodesmus subspicatus, former Scenedesmus subspicatus)
- Frequency: at least three times a week.

ACCLIMATION
Not necessary because the daphnids were bred in the laboratory under the same conditions as in the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

not applicable

Hardness:

14°dH (250 mg/L CaCO3)

Test temperature:

20.1 – 21.4 °C

pH:

6.70 – 8.33

Dissolved oxygen:

8.1 – 8.9 mg/L

Salinity:

not applicable

Nominal and measured concentrations:

0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: four 100 mL glass beakers per concentration, each filled with 50 mL
- Aeration: none
- Test type: static
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod / 8 hours darkness daily

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0

RANGE FINDING TEST:
The non-GLP range finding test was conducted with the following concentrations: 0, 0.10, 1.00 and 10.0 mg/L. As no immobilisation above the allowed control immobilisation of 10 % was observed up to 10.0 mg/L, the main test concentrations were chosen to be 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

Potassium Dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

31 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

In order to check the validity of the results, the toxicity of the reference item potassium dichromate was tested at 1.0 and 2.0 mg/L with 20 test organisms per test concentration on 26 May 2015 around the same time period as the study with the test item.
The results indicate an EC50 (24 h) of the reference item potassium dichromate between 1.0 and 2.0 mg/L. Since the results fall within the range recommended by the guideline (OECD 202), the daphnids were suitable for the determination of the toxicological effects of the test item.

After 24 and 48 hours no immobilisation was observed in controls and at 6.25 25.0 mg/L. After 24 hours of exposure 10 % immobilisation was observed at 12.5 mg/L, 50.0 mg/L had 75 % and 100 mg/L 90 % immobilisation.

After 48 hours of exposure immobilisation was still 10 % at 12.5 mg/L. At 25.0 mg/L the immobilisation was 15 %, at 50.0 mg/L 90 %, and at 100 mg/L 100 %. The results are presented inTable 1 andTable 2.

 

Table 1:   Results of the main test, 24 h values

Concentration Test Item	Control	6.25	12.5	25.0	50.0	100
		[mg/L]
	immobilised daphnids after 24 h
Group 1	0	0	1	0	3	4
Group 2	0	0	0	0	5	5
Group 3	0	0	0	0	5	5
Group 4	0	0	1	0	2	4
Σ	0	0	2	0	15	18
%	0	0	10	0	75	90

Table 2:     Results of the main test, 48 h values

Concentration Test Item	Control	6.25	12.5	25.0	50.0	100
		[mg/L]
	immobilised daphnids after 48 h
Group 1	0	0	1	0	4	5
Group 2	0	0	0	1	5	5
Group 3	0	0	0	1	5	5
Group 4	0	0	1	1	4	5
Σ	0	0	2	3	18	20
%	0	0	10	15	90	100

Validity criteria fulfilled:

yes

Conclusions:

According to the results of the test, the EC50 (48 h) for 2',4-Bis(methoxycarbonyloxy)-2-brom-acetophenone was determined to be 31.0 mg/L (nominal). The corresponding NOEC (48 h) was 12.5 mg/L (nominal).

Executive summary:

The toxicity of on Daphnia magna 2',4'-Bis(methoxycarbonyloxy)-2-brom-acetophenone was tested in a 48 hour acute immobilisation test. The test was performed static according to OECD Guideline 202 ("Daphnia sp., Acute Immobilisation Test") with concentrations of 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L and twenty organisms per test concentration (4 replicates of 5 animals each) were used.

According to the results of the test, the EC50 (48 h) for immobilisation was determined to be 31.0 mg/L. Tjhe corresponding NOEC (48 h) was 12.5 mg/L.

Description of key information

According to the results of the test, the EC50 (48 h) for 2',4-Bis(methoxycarbonyloxy)-2-brom-acetophenone was determined to be 31.0 mg/L (nominal). The corresponding NOEC (48 h) was 12.5 mg/L (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

31 mg/L

Additional information

The toxicity of on Daphnia magna 2',4'-Bis(methoxycarbonyloxy)-2-brom-acetophenone was tested in a 48 hour acute immobilisation test. The test was performed static according to OECD Guideline 202 ("Daphnia sp., Acute Immobilisation Test") with concentrations of 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L and twenty organisms per test concentration (4 replicates of 5 animals each) were used.

According to the results of the test, the EC50 (48 h) for immobilisation was determined to be 31.0 mg/L. Tjhe corresponding NOEC (48 h) was 12.5 mg/L.