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EC number: 212-079-0 | CAS number: 760-23-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Genotoxicity (in vitro)
3,4-dichlorobut-1-ene has been tested for bacterial reverse mutation in Salmonella typhimurium and Escherichia coli with and without an exogenous metabolic activation by OECD TG 471 test. This chemical shows mutagenicity only in TA1535 at 1000 µg/plate, but not in other Salmonella typhimurium strains or Escherichia coli strain. In the case of TA1535, the number of induced colonies/plate, 20- 30/mg-dose, indicates that this chemical is weakly mutagenic [MHW, Japan (1996)]. This study was identified to be a key study because it was well conducted and reported. The in vitro mammalian cell gene mutation test (HPRT assay) was conducted using Chinese Hamster Ovary cells with and without metabolic activation (OECD TG 476). The positive result was observed only without metabolic activation. [Du Pont, Haskell Laboratory, USA (1980)]. In the chromosomal aberration test (OECD TG 473), this chemical induced structural chromosome aberrations and polyploidy in Chinese hamster lung cells (CHL/IU) with and without metabolic activation at the 50% growth inhibition - concentrations of 0.01 and 0.2 mg/mL, respectively [MHW, Japan (1996)]. Among these studies MHW study was identified to be a key study because it was well conducted and reported. On the basis of these data, it can be concluded that 3,4-dichlorobut-1-ene is mutagenic in bacterial and mammalian cells and induced chromosomal aberrations in mammalian cells in vitro assays.
Genotoxicity (in vivo)
There are not studies available which have been performed according to the OECD test guidelines but there studies which give sufficient information to evaluate this endpoint.
3,4-dichlorobut-1-ene induced chromosomal aberrations in rat bone marrow cells after inhalation exposure for 30 and 120 days (4 hours/day, 5 days/week). The concentration of 13.7 and 81.3 mg/m³ caused chromosome damages, mainly of the chromatid type. [Nalbandyan, T. I. et al. (1985)] Another positive cytogenetic study is reported at the concentration of 13.9 and 107.8 mg/m³ in the bone marrow. [Gizhalaryan, M. S. et al. (1984)].
At concentration of 0.01 or 0.1 mg/kg 3,4 -dichlorobut-1-ene was found positive in an in vivo chromosome aberration test.
[Gizhalaryan, M. S. et al. (1984)]
On the basis of these data, it can be concluded that 3,4-dichlorobut-1-ene is clastogenic in rat bone marrow in vivo assays.
Short description of key information:
-Bacterial Test (gene mutation; S. typhimurium and E.coli; OECD 471 and Japanese guide line): + with and without metabolic activation;
-Non-Bacterial In Vitro Test (Chromosomal aberrations; CHL cells, OECD 473 and Japanese guideline): + with and without metabolic activation;
- In vitro cell gene mutation test (HPRT, CHO cells, OECD TG 406): positive result was observed only with metabolic activation;
-3 Cytogenetic (chromosome aberration assay in rat bone marrow: after inhalation and oral exposure.
Endpoint Conclusion: Adverse effect observed (positive)
Justification for classification or non-classification
On the basis of these results 3,4 -dichlorobut-1 -ene is to be classified as Xn, R68 according to DSD classification criteria and Muta. 2 (H341), according to the CLP classification criteria.
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