Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: -
CAS number: -
This study was performed to assess E stage 3 intermediate for its ability to cause gene mutations in vitro cultured mammalian cells L5187Y mouse lymphona cells (TK± -3.7.2C) after being exposed for 3 hours with and without metabolic action and 24 hours without metabolic action respectively using microwell format method. The method was designed to be compatible wth PRC-MEP, The Guidelines for the Testing of Chemicals (Health Effect, 2nd Edition), 476 'In vitro Cell Gene Mutation Test (2013)'.
L5178Y cells were exposed at 6 doses of 30, 30, 10, 5, 2.5, and 1.25 μg/ml in 3 treatment conditions. The positive and solvent (DMSO) controls were included at the same time in each treatment. The dose volume of each dose group and solvent control were 5μg/ml medium in duplicate. Plating Efficiency (PE) of the cells after exposure was determined and the Relative Total Growth (RTG) was calculated to evaluate toxicity. The Mutant Frequency (MF), Induced Mutant Frequency (IMF) of each culture and the Induced Mutant Frequency of small clone (IMFSC) of the highest dose (10 μg/ml ) and all controls were determined after the inhibition with Trifluorothymidine (TFT). In this test, the results of the solvent and positive control met all validity criteria so the sensitivity of the assay and efficacy of the S9 mixture were validated.
In three treatment conditions, no test item precipitate was observed in any cell culture at all doses before and after incubation.
The RTG of the cells exposed for 3 hours in the absence of S9 mix at the doses of 20, 10, 5, 2.5, 1.25μg/ml were 26%, 91%, 97%, 100% and 86%. The RTG of the cells exposed for 3 hours in the presence of S9 at the doses of 30, 20, 10, 5, 2.5, 1.25μg/ml were 14%, 54%, 85%, 86%, 84% and 103% .The RTG of the cells exposed for 24 hours in the absence of S9 mix at the doses of mix at the doses of 20, 10, 5, 2.5, 1.25μg/ml were 1%, 22%, 60%, 81% and 91%
The results of mutant frequency showed that the IMF of all cells cultures at all doses exposed for 3 and 24 hours in the presence and absence of S9 were less than 126 x 10 6.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Sellel veebilehel kasutatakse küpsiseid, et tagada lehe parim kasutus.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again