α-acetyl-γ-butyrolactone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001-01-25 to 2001-03-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

-

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

α-acetyl-γ-butyrolactone

EC Number:

208-235-2

EC Name:

α-acetyl-γ-butyrolactone

Cas Number:

517-23-7

Molecular formula:

C6H8O3

IUPAC Name:

3-acetyloxolan-2-one

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 d

Results and discussion

Effect concentrations

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

300 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 120 - 800 mg/l

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of 2-acetylbutyrolactone to Daphnia magna was determined in a 24 hour static test according to OECD guideline 202. Based on mean measured concentration, the 24-h EC50 was determined to be 300 mg/L with a 95% confidence interval ranging from 120 to 800 mg/L. The results of the test can be considered reliable without restriction.

Executive summary:

A range-finding test was followed by a final EC50 test performed under static conditions for 24 hours of exposure. The range-finding test showed a 24 EC50 value between 100 and 1000 mg/l.

In the final EC50 test Daphnia were therefore exposed for a maximum of 24 hours to nine different concentrations (5000, 2500, 1250, 625, 320, 160, 80, 40, 20, 0 mg/l)

The study included a blank-control. The test was performed in quadruplicate with 5 daphnia

per vessel. The 24h EC50 was determined to > 300 mg/l (95% CL: 120 - 800 mg/l)

24 h EC50 (reference substance) = 1.82 mg/l