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EC number: 206-581-9
CAS number: 355-37-3
Table 4. Main Study - Body Weights and Skin Reactions
Body wt (g)
- Grading erythema and eschar formation (Left = dorsal surface of left
ear; right = dorsal surface of right ear): 0 = No erythema
Table 5. Main Study - Relative Size Lymph Nodes, Radioactivity Counts (DPM) and Stimulation Index (SI)
DPM ± SEM3
SI ± SEM
691 ± 88
1.0 ± 0.2
545 ± 84
0.8 ± 0.2
720 ± 119
1064 ± 159
1.5 ± 0.3
- Relative size auricular lymph nodes (-, -- or ---: degree of
reduction, +, ++ or +++: degree of enlargement, n: considered to be
- DPM = Disintegrations per minute
- SEM = Standard Error of the Mean
Table 6. Reliability Check Results for Alpha- Hexylcinnamaldehyde (HCA; CAS no. 101-86-0)
DPM ± SEM
603 ± 123
1.0 ± 0.3
868 ± 111
1.4 ± 0.3
900 ± 97
2566 ± 300
4.3 ± 1.0
- Five females per group
- Acetone/Olive oil
a key in vivo guideline (OECD 429) study, the skin sensitisation
potential of the test material (1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane;
was evaluated in a Mouse Local Lymph Node Assay (LLNA).
material concentrations selected for the study were based on the results
of a pre-screen test. In this LLNA study, three experimental groups of
five female CBA/J mice were treated with the test material at
concentrations of 25, 50 or 100% w/w on three consecutive days, by open
application on the ears. Five vehicle control animals were similarly
treated, but with the vehicle alone (N,N-Dimethyl
formamide).Three days post the last exposure, all animals were injected
with 3H-methyl thymidine and after five hours the draining (auricular)
lymph nodes were excised and pooled for each animal.
precipitating the DNA of the lymph node cells, radioactivity
measurements were performed. The activity was expressed as the number of
disintegrations per minute (DPM) and a stimulation index (SI) was
subsequently calculated for each group.
irritation was observed in any of the animals. All auricular lymph nodes
of the control animals and animals treated with test material at
concentrations of 25% and 50% were considered normal in size. The nodes
of the animals treated with 100% test material were considered to be
enlarged. No macroscopic abnormalities of the surrounding area were
noted for any of the animals.
DPM/animal values for the experimental groups treated with the test
material at concentrations of 25, 50 and 100% were 545, 720, and 1064
DPM, respectively. The mean DPM/animal value for the vehicle control
group was 691 DPM. The SI values calculated for the test material at
concentrations of 25, 50 and 100% were 0.8, 1.0, and 1.5, respectively.
there was no indication that the test material elicited a SI ≥ 3 when
tested up to 100%, it was not considered to be a skin sensitizer.
six-month reliability check with Alpha-hexylcinnamaldehyde indicates
that the Local Lymph Node Assay as performed is an appropriate model for
testing for contact hypersensitivity.
on these results,1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane(AsahiklinTM
would not be regarded as a skin sensitizer according to the
recommendations made in the test guidelines. The test material does not
have to be classified and has no obligatory labelling requirement for
sensitization by skin contact according to the Globally Harmonized
System of Classification and Labelling of Chemicals (GHS) of the United
Nations (2015) (including all amendments) and the Regulation (EC) No
1272/2008 on classification, labelling and packaging of items and
mixtures (including all amendments).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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