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EC number: 206-581-9
CAS number: 355-37-3
Table 2. Mean absorption in the in vitro skin irritation test with Asahiklin™ AC-2000
= optical density
= Standard deviation
exposures are indicated by A, B and C.
this table, the values are corrected for background absorption (0.0408).
Isopropanol was used to
the background absorption.
Table 3. Mean tissue viability in the in vitro skin irritation test with Asahiklin™ AC-2000
Mean tissue viability
(percentage of control)
a key in vitro skin irritation test using a human skin model, the
test material (Asahiklin™ AC-2000; purity 100%) was applied undiluted
(25 µL) directly on top of the skin tissue (human three dimensional
epidermal model (EPISKIN Small Model (EPISKIN-SMTM))) for 15
± 0.5 minutes. After a 42-hour
post-incubation period, determination of the cytotoxic (irritancy)
effect was performed.
is expressed as the reduction of mitochondrial dehydrogenase activity
measured by formazan
production from MTT at the end of the treatment. Skin irritation is
expressed as the remaining cell viability after exposure to the test
relative mean tissue viability obtained after 15 ± 0.5 minutes treatment
with Asahiklin™ AC-2000 compared to the negative control tissues was
119%. Since the mean relative tissue viability for Asahiklin™ AC-2000
was above 50% after 15 ± 0.5 minutes treatment, Asahiklin™ AC-2000 is
considered to be non-irritant.
positive control had a mean cell viability of 44% after 15 ± 0.5 minutes
exposure. The absolute mean
OD570 (optical density at 570 nm) of the negative control
tissues was within the laboratory historical
control data range. The standard deviation value of the percentage
viability of three tissues treated
identically with the positive and negative control was less than 8%,
indicating that the test system
was concluded that Asahiklin™ AC-2000 is non-irritant in the in
vitro skin irritation test under the experimental conditions
described in this report.
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