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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994-01-31 to 1994-02-02
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: Japanese Industrial Standard (JIS K 0102-1986-71.), 'Testing methods for industrial waste water, Acute toxicity test with fish.
Deviations:
not specified
Principles of method if other than guideline:
The test was performed in accordance with Japanese Industrial Standard (JIS K 0102-1986-71.), 'Testing methods for industrial waste water, Acute toxicity test with fish.
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Asahi Glass Co., Ltd. (Japan); Batch no. 930817
- Expiration date of the lot/batch: not specified
- Purity test date: 1993-12-20 (before initiation of exposure)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: cold dark storage place
- Stability under test conditions: stable during exposure period

FORM AS APPLIED IN THE TEST (if different from that of starting material): Colourless and Transparent Liquid
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 125 mg/L
- Sampling method: Test substance and Dispersant (MEGAFAC F-142D; 10 times amount of the test substance) were mixed. The mixture was then diluted with ion-exchanged water to prepare 1000 mg/L stock solution.
- Sample storage conditions before analysis: Stock solution refrigerated and supplied to test tank
Vehicle:
yes
Remarks:
ion-exchanged water
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance and Dispersant (MEGAFAC F-142D; 10 times amount of the test substance) were mixed. The mixture was then diluted with ion-exchanged water to prepare 1000 mg/L stock solution.
- Controls: not specified
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dispersant: MEGAFAC F-142D
Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM
- Common name: Orange-red killifish
- Strain: not specified
- Source: Nakshima Fish Farm (Japan)
- Age at study initiation (mean and range, SD): Not specified
- Length at study initiation (length definition, mean, range and SD): Average length: 2.9 cm
- Weight at study initiation (mean and range, SD): Average weight: 0.22 grams
- Method of breeding: not specified
- Maintenance of the brood fish: Fish were visually examined upon receipt and those demonstrating any abnormalities were removed. The fish were reared for 3 days in a flow through system following an external disinfection.

ACCLIMATION
- Acclimation period: After rearing, fish were transferred to an acclimatiing aquarium and acclimatized there after the second external disinfection. Fish demonstrating any abnormalities were removed and reared for 12 days in a flow through system at a temperature of 25 ± 2°C. Fish were then checked for health conditions and reared for another 39 days after the external disinfection.
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: not specified
- Feeding frequency during acclimation: not specified
- Health during acclimation (any mortality observed): not specified
Test type:
semi-static
Water media type:
other: Underground water from the premises of Kurume Research Laboratories
Total exposure duration:
48 h
Test temperature:
25 ± 2°C
pH:
At initial exposure = 7.9
At termination of exposure = 7.3
Dissolved oxygen:
At initial exposure = 7.8 mg/L
At termination of exposure = 6.0 mg/L
Nominal and measured concentrations:
125 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass Gallon Bottle
- Type (delete if not applicable): open / closed (not specified)
- Renewal rate of test solution (frequency/flow rate): every 8-16 hours
- No. of organisms per vessel: 10/level

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Underground water from the premises of Kurume Research Laboratories
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 125 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

Cumulative Mortality

Concentration (mg/L)

Cumulative Mortality (%)

2 hours

24 hours

48 hours

Control

-

0

0

125

-

0

0

Validity criteria fulfilled:
not specified
Conclusions:
The 48-hour LC50 of the test material in Oryzias latipes was >125 mg/L.
Executive summary:

The 48-hour LC50 of the test material in Oryzias latipes was >125 mg/L.

Description of key information

The 48-hour LC50 of 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane in Oryzias latipes was determined to be >125 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
125 mg/L

Additional information

In a key short-term toxicity study in fish (Kurume Research Laboratories, 1994b), conducted in accordance with Japanese Industrial Standard (JIS K 0102-1986-71), Orange-red killifish (Oryzias latipes) were exposed to 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane at concentrations of 125 mg/L (mixed with dispersant (MEGAFAC F-142D) and diluted with ion-exchanged water) for a period off 48 hours.

 

The 48-hour LC50 of 1,1,1,2,2,3,3,4,4,5,5,6,6-tridecafluorohexane in Oryzias latipes was determined to be >125 mg/L.

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