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EC number: 276-123-0 | CAS number: 71873-46-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From April 06th to September 27th, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted April 4, 1984
- Deviations:
- yes
- Remarks:
- less concentrations were run
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The test was run with aerobic activated sludge from a domestlc waste-water treatment plant (ARA, Sissach/Switzerland).
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- pH:
- Control: 7.7- 7.8 (start)
Test vessels: 7.7 (start) - Nominal and measured concentrations:
- Nominal 10 and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass-flask (> 500 ml).
- Aeration: flasks were aerated with 0.2 l/minute instead of 0.5 l/minute to prevent foam development.
- No. of vessels per concentration: 1 replicate.
- No. of vessels per control: 2 replicates.
- No. of vessels per positive control: 1 replicate.
MEASUREMENTS
After 180 ninutes of aeration, the flrst sample was taken for oxygen measurement over a period of 10 minutes. Thereafter, in 15 minutes intervals, the rest of the samples were submitted to measurement of oxygen concentration.
Sampling was started wlth the control 1; thereafter, the oxygen consumptlin of the positive control, the test article samples and of control 2 was determined.
DETERMINATION OF OXYGEN CONCENTRATION
Oxygen consumption was measured over a period of 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
EFFECT PARAMETERS MEASURED
The inhibitory effect of the test article on the respiration rate (oxygen consumption per minute) is expressed as percentage of the mean of the two control respiration rates.
VALIDITY OF THE TEST
The tests are validated by testing a known standard, i.e. 3,5-dichlorophenol. The IC50 value should be in the range of about 5 - 30 mg/l.
Furthernore, the variation between the two control samples (start, end) should not be greater than 15 %. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol sodium salt (conc. 3.2, 10 and 50 mg/l)
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Due to aeration of the test medium wlth test article, a foamy solution was obtained, where mainly the foam was coloured. Under these conditions the respiration rate of the sludge was moderately inhibited (37.3 %) at the highest concentration of 100 mg/l.
- Results with reference substance (positive control):
- The respiration rate was slightly inhibited (12.4 %) at a concentration of 3.2 mg/l. The IC50 was found to be about 8.3 mg/l after 3 hours of exposure.
- Conclusions:
- IC50 (3h) > 100 mg/l (nominal)
- Executive summary:
Acute toxicity to waste-water bacteria was investigated following the OECD 209 testing guideline. The method deternines the respiration rate (oxygen consumption) of an aerobic actlvated sludge fed with an excess but standard amount of biodegradable food as compared to the respiration rate obtained in an activated sludge run under identical conditions but containing the test chemical.
The nominal test concentrations applied were 10 and 100 mg/l.
Due to aeration of the test medium wlth test article, a foamy solution was obtained, where mainly the foam was coloured. Under these conditions the respiration rate of the sludge was moderately inhibited (37.3 %) at the highest concentration of 100 mg/l.
Conclusion
IC50 (3h) > 100 mg/l (nominal)
Reference
Influence of test item and reference substance on the oxygen consumption of activated sludge, after 3 hours of exposure
Concentration (mg/l) | Oxygen consumption (mgO2/l/min) | Inhibition (%) | Starting pH |
Control | 0.94 | -- | 7.7 |
Control | 1.07 | -- | 7.8 |
Mean± s | 1.01± 0.07 | ||
Test item 100 | 0.63 | 37.3 | 7.7 |
Test item 10 | 0.92 | 8.5 | 7.7 |
Reference substance 50 | 0.09 | 91.0 | 7.7 |
Reference substance 10 | 0.25 | 75.1 | 7.7 |
Reference substance 3.2 | 0.88 | 12.4 | 7.7 |
Description of key information
IC50 (3h) > 100 mg/l (nominal)
Key value for chemical safety assessment
Additional information
Acute toxicity to waste-water bacteria was investigated following the OECD 209 testing guideline. Due to aeration of the test medium wlth test article, a foamy solution was obtained, where mainly the foam was coloured. Under the tested conditions, the respiration rate of the sludge was moderately inhibited (37.3 %) at the highest concentration of 100 mg/l.
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