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EC number: 272-554-3 | CAS number: 68877-27-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
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- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
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- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Zinc sulphide
- EC Number:
- 215-251-3
- EC Name:
- Zinc sulphide
- Cas Number:
- 1314-98-3
- Molecular formula:
- SZn
- IUPAC Name:
- thioxozinc
- Details on test material:
- ZnS-SACHTOLITH HD-S; lot no 2230100; purity 98.3% +/- 0.6%; expiration date: 7 May 2003; storage conditions: at room temperature at about 20°C away from direct sunlight.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The following samples were taken:
Just before the start: duplicates amples from the test medium (without algae) and duplicate samples from the control (without algae);
After 72 hours: duplicate samples from the test medium (without algae) and duplicate samples from the control (without algae).
All samples were deep-frozen immediately after sampling. The concentration of the test item was analyzed in the duplicate test medium samples of the single test concentration for both sampling times (0 and 72h). From the control samples only one of the duplicate samples was analyzed from each of both sampling times.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- synthetic test water prepared according to test guidelines. Test was started by inoculation of 10000 algal cells per ml test medium.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Strain No. 86.81 SAG supplied by the Sammlung von Algenkulturen Göttingen, Germany. The algae had been grown in the lab under standard conditions according to test guidelines.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22°C
- pH:
- start: 8.1
end: 9.3 - Dissolved oxygen:
- /
- Nominal and measured concentrations:
- Loading rate of 100mg/l translated into a mean measured test item concentration of 0.13 mg/l.
- Details on test conditions:
- Due to the insoluble properties of the test item, the study was performed as a limit test, using an undiluted filtrate of a supersaturated dispersion with the maximum concentration of dissolved test item as the only test concentration. The loading rate of 100mg/l was prepared by weighing 220 mg of the test item into 2200 ml test water. No auxiliary solvent or emulsifier was used. The test item was mixed into the test water as homogeneously as possible by ultrasonic treatment for 15 minutes and by intense stirring for 3 hours at room temperature in the dark to dissolve a maximum concentration of the test item in the dispersion. Then the solution was filtered through a membrane filter (0.45 µm) just before the start of the test.
The stirring period of 3h was chosen according to the results of a pre-test (without GLP) which showed that the solution equilibrium was reached after this time. The undiluted filtrate was used as test medium.
The test concentration was based on the results of a range-finding test and on results of a pre-experiment to the solubility of the test item.
The test method is also based on the OECD Guidance document on Aquatic Toxicity testing of Difficult Substances and Mixtures (OECD, 2000).
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 13 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: dissolved concentration corresponding to 100mg/l loading of ZnS
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 13 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: dissolved concentration corresponding to 100mg/l loading of ZnS
- Details on results:
- No effects were observed at the loading rate of 100mg/l of the substance (ZnS), corresponding to 13µg Zn/l in solution. Therefore the EC50 is expressed as ">".The 72h NOEC may have been higher than 13 µg/l but the loading rates in excess of 100 mg/l were not tested in accordance to the test guidelines.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- test valid for assessing acute toxicity of the substance to algae; results demonstrate no toxicity accoring to classification criteria
- Executive summary:
A standard OECD 202 test on Scenedesmus subspicatus showes that at loading of 100mg substance/l, no effects are observed.
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