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EC number: 692-713-4
CAS number: 886588-62-1
The Ames II assay is a high throughput version showing a high
correlation with the traditional Salmonella assay. Concurrently with
positive and negative (vehicle) controls, the test article was tested
over a concentration range from 1 to 2500 μg/mL medium with and without
microsomal rat liver enzymes (Aroclor 1254-induced). The bacterial
tester strains S. typhimurium TA Mix (TA 7001, TA 7002, TA 7003, TA
7004, TA 7005 and TA 7006, sensitive to base-pair substitution) and TA
98 (susceptible to frameshift mutagens) are histidine-auxotrophic and
were exposed with the test substance for 90 minutes. Using the liquid
fluctuation technique revertant growth was quantified colorimetrically
in 384-well plates after 48 hours at 37°C.
Solubility and Toxicity
CD 713 TD showed neither precipitation nor bacteriotoxicity up to the
highest soluble concentration of 2500 μg/mL. The highest planned test
concentration of 5000 μg/mL was not soluble in DMSO.
CD 713 TD did not increase consistently the number of positive wells in
the different tester strains with and without S9 mix as compared to the
vehicle control. The negative control showed a mean number of positive
wells for S. typhimurium TA Mix (TA 7001- 7006) and TA 98 similar to
those described in the literature (≤8/48 wells).
Based on the described results it is concluded, that CD 713 TD, when
tested up to insoluble concentrations, caused neither base-pair
substitutions nor frameshift mutations in bacteria. No evidence of
genotoxic activity was observed in a series of S. typhimurium tester
strains (TA Mix and TA 98) in the absence and presence of metabolic
activation. The test article is, therefore, classified as "Ames II
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