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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not to GLP, no timeframe or dosing schedule reported. Follows basic scientific principles.

Data source

Reference
Reference Type:
publication
Title:
Behavioural and toxic profiles of some essential trace metals salts in mice and rats
Author:
Singh PP and Junnarkar AY
Year:
1991
Bibliographic source:
Indian Journal of Pharmacology, 23(3): 153-159

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
In all tests trace metal salts were dissolved in deionised triple glass distilled water and were administered by ip, po and iv routes.

GROSS BEHAVIOURAL EFFECT STUDY: 1/10 of LD50 dose was administered by the p.o route. The gross behavioural changes were recorded at 30, 60, 120 and 240 mins. The changes in rectal temperature were recorded by using the thermocouple probes on the Digital precision thermometer - DU - 3S,. Behavioural effects were scored on a scale of 0-8 were 4 was normal, <4 indicated a decrease in activity and > 4 indicated an increase in activity. For body temperature the normal score was 0.

PENTOBARBITONE-INDUCED HYPNOSIS IN RATS: Salts were administered at 1/10th of LD50 dose one hour before pentobarbitone sodium (40 mg / kg ip). The time lapse from the loss and gain of righting reflex was calculated and expressed as sleeping time,.
GLP compliance:
not specified
Test type:
other: acute toxicity, behavioural parameters assessed

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
No data

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals and environmental conditions:
Animals were kept in polycarbonate cages at an ambient temperature of 24 ± 0.5°C, maintaining a 12 hour dark and 12 hour light cycle. Animals were fed Gold Mohur Hind Lever diet and give water ad libitum.

Administration / exposure

Route of administration:
other: oral gavage, i.p and i.v
Vehicle:
water
Doses:
No data
No. of animals per sex per dose:
No data
Control animals:
yes

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LD50
Effect level:
2 150 mg/kg bw
Remarks on result:
other: MnSO4
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 470 mg/kg bw
Remarks on result:
other: MnCl2
Mortality:
No data
Clinical signs:
MnSO4 did not have any effect of behavioural parameters.
Body weight:
No data
Gross pathology:
No data
Other findings:
No data

Any other information on results incl. tables

Salt

LD50 mg / kg in rat

p.o.

i.p.

i.v.

MnSO4

2150.0

92.6

44.1

MnCl2

1470.0

147.0

92.6

Applicant's summary and conclusion

Conclusions:
MnSO4 is not acutely toxic under the conditions of this study.