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Diss Factsheets
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EC number: 200-262-8 | CAS number: 56-23-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: A scientifically sound study but no individual data, no necroscopy or histological data.
Data source
Reference
- Reference Type:
- publication
- Title:
- Comparative percutaneous toxicity of ten industrial solvents in the guinea pig
- Author:
- Wahlberg, J., E.; Boman, A.;
- Year:
- 1 979
- Bibliographic source:
- Scand. j. work environ. & health, vol. 5, p. 345-351
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- 0.5 and 2.0 mL of CTC were applied via intraperitoneal injection to guinea pigs weighting 372 ± 3 g (low dose group) and 373 ± 6 g (high dose group); the mortality rate was determined after an observation period of 35 d
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Carbon tetrachloride
- EC Number:
- 200-262-8
- EC Name:
- Carbon tetrachloride
- Cas Number:
- 56-23-5
- Molecular formula:
- CCl4
- IUPAC Name:
- tetrachloromethane
- Details on test material:
- - Name of test material (as cited in study report): carbon tetrachloride
- Physical state: not reported
- Analytical purity: p. a.
- supplier: Merck, Darmstadt, Germany
- Stability under test conditions: not reported
- Storage condition of test material: not reported
Constituent 1
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: not reported
- Age at study initiation: not reported
- Weight at study initiation: 372 ± 3 g low dose group, 373 ± 5 g high dose group
- Fasting period before study: not reported
- Housing: in groups of three in Makrolon cages
- Diet (e.g. ad libitum): standard laboratory animal food (Astra Ewos, Sodcttalje, Sweden), ad libitum
- Water (e.g. ad libitum): water supplemented with ascorbic acid, ad libitum
- Acclimation period: not reported
ENVIRONMENTAL CONDITIONS
- Temperature (°C): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- unchanged (no vehicle)
- Details on exposure:
- 0.5 and 2.0 mL of CTC were applied via intraperitoneal injection to guinea pigs weighting 372 ± 3 g (low dose group) and 373 ± 6 g (high dose group); the mortility rate was determined after an observation period of 35 d, control animals were injected with distilled water
- Doses:
- - 0.5 and 2.0 ml (= 0.792 and 3.17 g, on average 2130 and 8500 mg/kg bw)
- No. of animals per sex per dose:
- 20 per dose, sex not indicated
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 35 days
- Frequency of weighing: daily, but not reported
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other: no - Statistics:
- - no statictic analysis conducted
Results and discussion
Effect levels
- Sex:
- not specified
- Dose descriptor:
- other: approximated LD50
- Effect level:
- > 2 130 - < 8 500 mg/kg bw
- Mortality:
- after 3 days: 2130 mg/kg bw 9/20
8500 mg/kg bw 10/20
after 35 days: 2130 mg/kg bw 9/20
8500 mg/kg bw 10/20 - Clinical signs:
- - not analyzed
- Body weight:
- - not repoted
- Gross pathology:
- - not analyzed
Applicant's summary and conclusion
- Conclusions:
- From the present study (Wahlberg, 1979) the LD50 (guinea pig, i.p. injection) can be approximated to lie between 2130 and 8500 mg/kg bw.
- Executive summary:
The potential of the test substance CTC (named carbon tetrachloride in the study report) to induce acute toxicity after intraperitoneal injection was evaluated in a study following no official guideline. Guinea pigs, unspecified concerning sex and strain, were injected i.p. with CTC and were observed for 35 days for mortality and body weight only, but the body weight development was not reported. No pathological or histological analysis were conducted. Treatment with the the test substance resulted in mortalities of 45 % at 2130 mg/kg bw and 50 % at 8500 mg/kg bw after 35 d of observation. All deaths occured within 3 days post exposure without any further deaths during the rest of the observation period. So this effect is monophasic in contrast to the occurence of deaths after dermal application which was biphasic. As only two concentration were tested which both lead to about 50 % mortality, the LD50 could only be approximated to lie between the two tested doses. The approximated LD50 was derived to be >2000 mg/kg bw.
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