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EC number: 228-408-6
CAS number: 6259-76-3
Toxicity to Aquatic Invertebrates
purpose of this study was to evaluate the acute toxicity of hexyl
salicylate to Daphnia magna in a 48 hour, semi-static study
with renewal of the test media after 24 hours. This study was carried
out to OECD Guideline 202. Nominal exposure concentrations were 0
(control), 0 (solvent control), 0.05, 0.1, 0.23, 0.5 and 1.0 mg/L, the
corresponding mean measured concentrations were 0.03, 0.072, 0.140,
0.310 and 0.726 mg/L. The results of the study are based on the mean
measured exposure concentrations. The 48 hour EC50 was calculated to
be 0.357 mg/L (with 95% confidence limits of 0.228 to 0.871 mg/L), the
corresponding no-observed effect concentration was 0.140 mg/L (Pavic
and Wydra 2008).
Toxicity to Fish
from two fish acute toxicity studies are presented: Danio rerio were
exposed to the hexyl salicylate at nominal test concentrations of: 0,
1, 10 and 100 mg/L. This study was carried out to OECD Guideline 203,
the study appears to have been conducted as a range-finding test with
a concentration spacing factor or 10 and only three concentrations
tested. Hexyl salicylate was tested over the limit of solubility,
without specific preparation and with no analytical verification. At
the end of the 96 hour exposure period there was 20% mortality in the
nominal 100 mg/L test treatment, therefore the 96 hour LC50 was
observed to >100 mg/L (Licata-Messana, 2000).
read-across report was also provided as support for this data point.
The acute toxicity of amyl salicylate toDanio reriowas
determined. Based on mean measured concentrations the 96 hour LC0 was
determined to be 0.95 mg/L, the corresponding 96 hour LC100 was
determined to be 1.90 mg/L. The 96 hour LC50 of 1.34 mg/L was
determined by dividing the 96 hour LC0 and LC100 values (Bruns et al,
1992). Due to the similar structural, physical and chemical properties
of amyl salicylate, it was considered appropriate for the purposes of
read-across to hexyl salicylate.
to Aquatic Algae
Two algal studies were performed, one with hexyl salicylate and the
other with Cis-3 -Hexenyl Salicylate as a read-across study. The
hexyl salicylate study was conducted with a wide spread of
concentrations, the highest two of which were above the expected water
solubility value, additionally no analytical verification was
performed. The Cis-3-Hexenyl Salicylate study was
conducted with a range of concentrations that were less than the limit
of water solubility and with analytical verification. Therefore
the results of the Cis-3-Hexenyl Salicylate study will be used to
provide the appropriate endpoint for algae. Due to the similar
structural and chemical properties of Cis-3-Hexenyl Salicylate, it was
considered appropriate for the purposes of read-across to Hexyl
Salicylate.Based on the Cis-3-Hexenyl Salicylate the 72 ErC50
of 0.61 mg/L is the appropriate endpoint.
data has been generated to fulfil this data requirement. However, a
biodegradation study with hexyl salicylate was conducted according to
OECD guideline 301F with a nominal concentration of 100 mg/L. Under
the conditions of the test, hexyl salicylate underwent 91 %
biodegradation after 28 days. Biodegradation reached 82 % at the end
of the 10-day window (days 2 to 12).
hexyl salicylate should be regarded as readily biodegradable according
to this test.
the concentration used in the test (100 mg/L), hexyl salicylate is not
inhibitory to the micro-organisms.
the absence of toxicity study conducted according to OECD guideline
209 the ECHA guidance document, Chapter R184.108.40.206 states the
following: “The assumption that the substance under investigation is
not inhibitory to the micro-organisms when dosed in the test system is
implicit in ready biodegradability testing (i.e. ... OECD 301F). If a
compound degrades well in a ready biodegradability test, or does not
inhibit the degradation of a positive control at a certain
concentration, this concentration can be used as a NOEC value”.
it is considered appropriate to set the NOEC for this endpoint as 100
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