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Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP guideline study - only 4 strains tested

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Principles of method if other than guideline:
The mutagenic potential of dimethyl propylphosphonate was examined in the salmonella/microsome test. Bacteria of four histidine-auxotrophic Salmonella typhimurium LT2 mutant strains (TA 98, TA 100, TA 1535, and TA 1537) were exposed to doses up to 5000 µg per plate.
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Dimethyl propylphosphonate
EC Number:
242-555-3
EC Name:
Dimethyl propylphosphonate
Cas Number:
18755-43-6
Molecular formula:
C5H13O3P
IUPAC Name:
dimethyl propylphosphonate
Test material form:
other: colourless liquid
Details on test material:
- Purity: 98.7 %

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Additional strain / cell type characteristics:
other: Firstly, they are deep rough since certain lipopolysaccharide side chains are missing in the bacterial cell wall. Secondly, their reduced ability to repair damage from UV light allows the phenotypic detection of mutation events
Metabolic activation:
with and without
Metabolic activation system:
S9 mix
Test concentrations with justification for top dose:
first and repeat test:
0, 8, 40, 200, 1000, 5000 µg/plate
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
sodium azide
other: nitrofurantoin, 4-nitro-1,2-phenylenediamin, 2-aminoanthracene

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid

Any other information on results incl. tables

Doses up to and including 5000 µg per plate did not cause any bacteriotoxic effects: Total bacteria counts remained unchanged

and no growth inhibition was observed.

There was no evidence for mutagenic effects of dimethyl propylphosphonate with and without S9 mix. A biologically relevant

increase of the mutant count over control levels was not observed. Therefore, dimethyl propylphosphonate was considered

to be non-mutagenic with and without S9 mix in the Salmonella/ microsome test.

The positive controls sodium azide, nitrofurantoin, 4-nitro- 1,2-phenylene diamine and 2-aminoanthracene revealed marked

mutagenic effects, as indicated by a biologically relevant increase of mutant colony numbers over colony numbers of the negative controls.

Applicant's summary and conclusion

Conclusions:
Interpretation of results:
negative
Executive summary:

The mutagenic potential of dimethyl propylphosphonate was examined in the salmonella/microsome test (Ames test). Bacteria of four histidine-auxotrophic Salmonella typhimurium LT2 mutant strains (TA 98, TA 100, TA 1535, and TA 1537) were exposed to doses up to 5000 µg per plate.

Dimethyl propylphosphonate was negative in the salmonella/microsome test with and without metabolic activation.