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EC number: 209-711-2 | CAS number: 591-27-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 h of incubation in the dark at 25 1°C, the root elongation of each seed was measured to 1 mm.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Toxicant test solutions were obtained from the stock solutions prepared by adding the accurately weighed chemicals to dilution water (deionized water) and stirring for a minimum of 20 h. Solution of title compound in water was applied.
- Species:
- Cucumis sativus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Common name: Cucumber
Plant family: Cucurbitaceae
Source of seed: Seeds purchased commercially
Prior seed treatment/sterilization: The seeds were sterilized with 0.1 % NaClO solution for 20 min. soaked for 10 min. and washed three times with deionized water before use.
Historical germination of seed (germination of seed lot tested): The seeds of Cucumis sativus (Sprout containing >= 95 %, purity >=95) - Test type:
- seed germination/root elongation toxicity test
- Study type:
- laboratory study
- Substrate type:
- filter paper
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 25+/- 1⁰C
- pH:
- 6.2
- Details on test conditions:
- Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 hr of incubation in the dark at 25+/- 1⁰C, pH 6.21 the root elongation of each seed was measured to 1 mm.
Range finding study: Range-finding tests were performed to determine the range of the test solutions and the dilution factors and to get information about the solubility and behavior of the compounds in aqueous solution. - Nominal and measured concentrations:
- Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration
- Reference substance (positive control):
- not specified
- Key result
- Species:
- Cucumis sativus
- Duration:
- 48 h
- Dose descriptor:
- other: RC50
- Effect conc.:
- 1 000 other: mg/l
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Root elongation inhibition
- Remarks on result:
- other: Reported as RC50
- Details on results:
- RC50: Root elongation half inhibition concentration
- Reported statistics and error estimates:
- RC50 (in mg/L), the concentration on which the average root length was half of the corresponding control for each chemical, was calculated by the log-linear regression analysis in Statistica and was transformed as the negative logarithmic form (mol/L) for each chemical as its toxic potency, expressed as log1/RC50
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Root elongation half inhibition concentration (RC50) of test chemical in Cucumis sativus after 48 hours of exposure was reported to be 1000 mg/L
- Executive summary:
The comparative inhibition effect of test chemical on the germination rate of cucumis sativus was determined. Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 h of incubation in the dark at 25 1°C, the root elongation of each seed was measured to 1 mm. After 48 h of incubation in the dark at 25 +/- 1 deg C, the root elongation of each seed was measured to 1 mm. Root elongation half inhibition concentration (RC50) was investigated after 48 hours of exposure. RC50 for test chemical was reported to be 1000 mg/L.
Reference
Description of key information
The comparative inhibition effect of test chemical on the germination rate of cucumis sativus was determined. Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 h of incubation in the dark at 25 ± 1°C, the root elongation of each seed was measured to 1 mm. After 48 h of incubation in the dark at 25 +/- 1 deg C, the root elongation of each seed was measured to 1 mm. Root elongation half inhibition concentration (RC50) was investigated after 48 hours of exposure. RC50 for 3-aminophenol was reported to be 1000 mg/L.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for terrestrial plants:
- 1 000 mg/kg soil dw
Additional information
Based on the various experimental data for the test chemical study have been reviewed to determine the mode of action of test chemical on the growth of terrestrial plants. The studies are as mentioned below:
In the first experimental key study from peer reviewed journal 2002, the comparative inhibition effect of test chemical on the germination rate of cucumis sativus was determined. Phytotoxicity was conducted according to OECD guidelines. The tests were conducted using 100 x 15 mm disposable petri dishes and Whatman No. 1 filter paper. Fifteen pretreated, undamaged, and plump seeds of almost identical size were placed evenly on the filter paper in each dish. Each dish was filled with 5 ml test solution or deionized water in the control. The dishes then were sealed with Parafilm laboratory film during the period of incubation to avoid the loss of chemicals due to volatility. Six concentrations in geometric series were set, ranging from no effect to 100% inhibition concentration, and four replicates were set for each concentration. Solutions were renewed every 12 h to achieve semistatic exposure. Deionized water without test compounds served as control. After 48 h of incubation in the dark at 25 ± 1°C, the root elongation of each seed was measured to 1 mm. After 48 h of incubation in the dark at 25 +/- 1 deg C, the root elongation of each seed was measured to 1 mm. Root elongation half inhibition concentration (RC50) was investigated after 48 hours of exposure. RC50 for test chemical was reported to be 1000 mg/L.
First study was supported by the second study from peer reviewed journal 2001. Seed germination rate inhibition studies were conducted in Cucumis sativus for 48 hours with different concentrations of test chemical. Comparative inhibition effect of selected nitrogen-containing aromatics on germination rate of Cucumis sativus was investigated in which 15 seeds were germinated in solution of test chemical for 48 h in dark at 25 deg C; pH 6.24; 6 concentrations ranging from no effect to 100 percent inhibition concentration were tested and the germination rate was calculated. The GC50 (concentration that inhibited germination rate by 50 percent) value of test chemical in Cucumis sativus was observed to be 1203.9 mg/l.
Based on the both studies chemical toxicity value ranges from 1000 mg/l to 1203.9 mg/l.
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