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Ecotoxicological information

Short-term toxicity to fish

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Link to relevant study record(s)

Description of key information

1) WoE: A short term fish test (K4) (48h) was performed with Orange killifish (Oryzias latipes) according to the Japanese Industrial standard (JIS K 0102-1993-71 & GLP); LC50 (48h) = 17.2 mg/L 
2) WoE: A short-term fish test (K2) (7d) was performed with Rainbow trout (Oncorhynchus mykiss), however not according to any specific guideline.
3) WoE: A prolonged fish toxicity test (14 days) (OECD 204 & GLP) (K1) with Rainbow trout (Oncorhynchus mykiss) provided valuable information on the acute toxicity; EC50 (4d, mortality) = 0.48 mg/L; NOEC (4d) = 0.14 mg/L (WoE)
4) WoE: A prolonged fish toxicity test (14 days) (OECD 204 & GLP) (K1) with Common Carp (Cyprinus carpio) provided valuable information on the acute toxicity; EC50 (4d, mortality) > 0.67 mg/L; NOEC (4d) = 0.28 mg/L (WoE)

Key value for chemical safety assessment

LC50 for freshwater fish:
0.48 mg/L

Additional information

There are two test reports available describing acute toxicity to fish (Tadokoro, 1998; Dionne, 1997). Both studies were performed as range-finder tests for long-term fish toxicity studies. Consequently, concentrations were not analytically confirmed for all test conditions and therefore results display a certain level of uncertainty. Besides the actual acute test reports, the prolonged fish tests (OECD 204) performed on Rainbow trout (Oncorhynchus mykiss) (Dionne, 1997) and common carp (Cyprinus carpio) (Dionne, 1995) also provided very useful information on the short term toxicity of 1,4-benzenediamine, N,N'-mixed Ph and tolyl derivs. since 96h-LC50 values are 0.48 and > 0.67 mg/L, while NOECs (96h) are 0.14 and 0.28 mg/L for trout and carp, respectively. The concentrations used in these tests were analytically verified and since both tests were performed according to the OECD and GLP guidelines, the test results gathered in these tests were used for further risk assessment and classification purposes.