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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Study period:
25 Aug - 08 Sep 1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given: comparable to standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Internal BASF-Test: comparable to standard methods
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Citral
EC Number:
226-394-6
EC Name:
Citral
Cas Number:
5392-40-5
Molecular formula:
C10H16O
IUPAC Name:
3,7-dimethylocta-2,6-dienal
Constituent 2
Chemical structure
Reference substance name:
(Z)-3,7-dimethylocta-2,6-dienal
EC Number:
203-379-2
EC Name:
(Z)-3,7-dimethylocta-2,6-dienal
Cas Number:
106-26-3
Molecular formula:
C10H16O
IUPAC Name:
(2Z)-3,7-dimethylocta-2,6-dienal
Details on test material:
3,7-Dimethyloctadien-2,6-al-1
Purity: >= 95 %

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Wiga
- Weight at study initiation: males 26 g, females 22 g

Administration / exposure

Route of administration:
intraperitoneal
Details on exposure:
Injection of 0.681 - 50 % emulsion in 0.5 % aqueous CMC-solution with 2-3 drops of Cremophor EL
Doses:
215, 147, 100 and 68.1 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Volume of application: 10 ml/kg bw
- Duration of observation period following administration: 14 days
- Frequency of observations: 15 and 30 min, 1, 2, 4, and 5 hours, 1, 4, 5, 6, 7, 8, 11, 12, 13 and 14 days
- Necropsy of survivors performed: yes
- Body weight: at the initiation of the study, at day 4, 7 and 14

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
147 - 215 mg/kg bw
Remarks on result:
other: mortality: 215 mg/kg: males 5/5, females 2/5; 147 mg/kg: 0/10
Mortality:
215 mg/kg bw; males: 5 out of 5 animals died during the study (1 animal at day 3, 4 animals at day 4); females: 2 out of 5 animals died during the study (both at day 4)
147, 100 and 68.1 mg/kg bw: no animal died during the study
Clinical signs:
215 and 147 mg/kg bw; The animals showed dyspnoe, apathy, tremor, spastic movement and poor general state during the first 24 hours after injection

100 and 68.1 mg/kg bw:
no symptoms detectable
Body weight:
Nothing abnormal detected
Gross pathology:
Dead animals: no findings;
Sacrificed animals: liver agglutination

Applicant's summary and conclusion

Conclusions:
After intraperitoneal application of citral (vehicle carboxymethyl cellulose) to mice, no mortalities were observed at a dose of 147 mg/kg, whereas 5/5 males and 2/5 females died at a dose of 215 mg/kg.
Executive summary:

After intraperitoneal application of citral (vehicle carboxymethyl cellulose) to NMRI mice (groups of 5 males and 5 females) at doses ranging from 68 to 215 mg/kg, no mortalities were observed at a dose of 147 mg/kg, whereas 5/5 males and 2/5 females died at a dose of 215 mg/kg.