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EC number: 235-935-5
CAS number: 13052-09-0
Respiration rate in O2/L/hr
Resp. rate (mg O2/L/hr
the request of ATOFINA, Paris-la-Defense, France, the effect of the test
item, 2,5-DIMETHYL-2,5-DI(2-ETHYLHEXANOYL PEROXY)HEXANE, on the
respiration of activated sewage sludge was evaluated using a 3-hour
static test according to OECD guideline (No. 209, 4th April 1984).
test solutions were prepared as follows:
control solutions: activated sludgeindechlorinated
water to obtain a final sludge concentration of 1.6 g/L,
test item solutions: activated sludge in dechlorinated water (at 1.6
g/L) and test item (to obtain final concentrations of 10, 31.6, 100, 316
and 1000 mg/L),
reference items (3,5-dichlorophenol) solutions: activated sludge in
dechlorinated water (at1.6 g/L)and reference item (to obtain final
concentrations of 4, 12 and 36mg/L).
oxygen consumption of these solutions was measured for approximately 10
minutes, with a chart recorder, after an aeration of 3 hours.
EC50 (concentration expected to cause 50% inhibition of the respiration
rate) of the test item and the reference item was determined considering
the oxygen consumption of the controls as 100
the validity criteria were met (the difference between the two controls
was below 15% and the EC50 of3,5-dichlorophenol was between 5 and 30mg/L),the
test was considered valid.
respiration rate of the test solution of highest concentration (1000mg/L)was
equivalent to the respiration rate of the first control (i.e. these
rates were within 15% of each other). Therefore, the oxygen consumption
rate of the four other test solutions (10 to 316mg/L)was
not determine EC50s,
based on nominal concentrations, were as follows:Test
item (3 hr EC50) > 1000 mg/LReference
item (3 hr EC50) = 10.9 mg/L
the experimental conditions, the 3-hour EC50 of the test item
2,5-DIMETHYL-2,5DI(2-ETHYLHEXANOYL PEROXY)HEXANE is > 1000 mg/L for
activated sludge. The test item should be considered as non-toxic for
the micro-organisms of a water treatment plant
One OECD 209 study is available and the 3 h EC50 of the test item
2,5-DIMETHYL-2,5-DI(2-ETHYLHEXANOYL PEROXY)HEXANE is > 1000 mg/L for
There is one OECD 209 study available
carried out under GLP. Under the experimental conditions, the 3-hour
EC50 of the test item 2,5-DIMETHYL-2,5-DI(2-ETHYLHEXANOYL PEROXY)HEXANE
is > 1000 mg/L for activated sludge.
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