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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- CAS No.: 078567-28-9; Batch No.: WDJ 1853D-3; Purity: weight percentage about 100 %;
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling schedule of chemical analysis
Control: at 0 and 48 hours
Test concentrations: at 0 and 48 hours - Vehicle:
- no
- Details on test solutions:
- Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9): 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin. This standard dilution water is used for both, the maintenance of the test animals and the preparation of stock and test solutions of the test substance.
Pretreatment oe the test substance
To give the desired series oftest concentrations, the test substance was added to 1 litre of dilution water for each test concentration, then treated with an ultra turrax for 60 seconds at 8000 rpm and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test substance were removed by filtration using folded filters of pore size 7-12 Ilm. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Name: Daphnia magna STRAVS, parthenogenetic females
Source: Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1°C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semiquantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 14.8°dH (= 264.2 mg/I CaC03)
- Test temperature:
- mean +/- 1 °C (in the temperature range 18-22 °C)
- Nominal and measured concentrations:
- Test concentration/s: (nominal) 25, 50 and 100 mg/I
- Details on test conditions:
- Exposure conditions
Test vessels: 50 ml glass beakers holding 10 neonates in 20 ml of test medium
Experimental design: 3 test concentrations plus 1 control; 10 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period
Method of initiation: neonates were placed in prepared media
Aeration: none
Photoperiod 16h light: 8 h dark
Method of administration: direct weighing
Criteria of effects: the criterion of adverse effects used in this study was the substance-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours. - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL0
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- ca. 58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The results are expressed in terms of effective loadings (EL) at 24h and 48h. Considering the heterogeneous composition ofthe test substance, Water Accommodated Fractions (= WAFs) were tested. The results of the TOC determinations reflect a rough impression only of exposure conditions over time. No informations are available on a potential correlation between effective loadings and measured TOC values.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the nominal EL50 of the test substance in Daphnia magna was determined to be 58 mg/L.
- Executive summary:
A study was conducted to determine the short term toxicity of the test substance to Daphnia magna STRAUS under static conditions according to EU Method C.2, in compliance with GLP. The Daphnia were exposed to a range of nominal concentrations: 25, 50 and 100 mg/L. The test substance was dispersed in water, mixed with an ultra turrax, a magnetic stirrer and finally filtered. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. Under the study conditions, the following values were determined: Highest test concentration resulting in 0% immobilisation (EC 0 48 h): 25 mg/L. Lowest test concentration resulting in 100% immobilisation (EC 100 48 h): > 100 mg/L. 40% and 90% immobilization was observed after 48 h at loading rates of 50 and 100 mg/L respectively. The results are expressed in terms of effective loadings (EL) at 24 h and 48 h. Under the study conditions, the nominal EL50 of the test substance in Daphnia magna was determined to be 58 mg/L (Bruns, 2002).
Reference
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 58 mg/L
Additional information
A study was conducted to determine the short term toxicity of the test substance to Daphnia magna STRAUS under static conditions according to EU Method C.2, in compliance with GLP. The Daphnia were exposed to a range of nominal concentrations: 25, 50 and 100 mg/L. The test substance was dispersed in water, mixed with an ultra turrax, a magnetic stirrer and finally filtered. Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. Under the study conditions, the following values were determined: Highest test concentration resulting in 0% immobilisation (EC 0 48 h): 25 mg/L. Lowest test concentration resulting in 100% immobilisation (EC 100 48 h): > 100 mg/L. 40% and 90% immobilization was observed after 48 h at loading rates of 50 and 100 mg/L respectively. The results are expressed in terms of effective loadings (EL) at 24 h and 48 h. Under the study conditions, the nominal EL50 of the test substance in Daphnia magna was determined to be 58 mg/L (Bruns, 2002).
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