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EC number: 947-738-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- September 2017 - February 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- July, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- yes
- Remarks:
- stock solutions were prepared with chlorine-free tap water
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant Staufener Bucht was used as test system. The treatment plant has a capacity of 140 000 inhabitant equivalents and clarifies predominantly domestic wastewater. The Inoculum was not adapted. The activated sludge was kept aerated until use at test temperature. Dry solid of the activated sludge was determined as 3.8 g/L (range-finder test) and 5.3 g/L (full test) by weight measurements before and after drying at 105°C (mean of triplicate measurements). The activated sludge was washed twice by settling the sludge, decanting the supernatant and re-suspending the sludge in chlorine-free tap water. Before using, the activated sludge was diluted to 3 g/L dry solids with chlorine-free tap water, in order to obtain a final concentration of 1.5 g/L dry solids in the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- N/A
- Post exposure observation period:
- N/A
- Hardness:
- 2.19 mmol/L
- Test temperature:
- 18.3-21.8 °C
- pH:
- 7.3-7.4
- Dissolved oxygen:
- N/A
- Salinity:
- N/A
- Conductivity:
- N/A
- Nominal and measured concentrations:
- Range-finder test with three test concentrations: 10, 100 and 1000 mg/L
Full test with six concentrations (nominal): 62.5, 125, 250, 500, 1000, 2000 mg/L - Details on test conditions:
- The different vessels were started separately with an interval of exactly 15 min in order to be able to measure the respiration rates one by one while making sure that the aeration time is 3 h in every vessel. At time "0", 16 ml synthetic sewage and the test item or reference compound were added to the test vessel and made up to 250 mL with chlorine-free tap water. After measurement and adjustment of pH, 250 mL of activated sludge (3 g/L dry solids) were added consequently obtaining a total volume of 500 ml with 1.5 g/L dry solids. For the controls the same procedure was made as with the test vessels, however without test
or reference substance. All vessels were stirred and aerated throughout the contact time with room air to assure an oxygen saturation of 60-70% using a membrane pump. Before starting the test the oxygen saturation rate was measured.
After 3 h the aeration of the first vessel was shut off and the respiration rate was measured in a BOD-bottle with an oxygen electrode while mixing with a magnetic stirrer.
This determination was repeated for each vessel at 15-min intervals, ensuring that the contact time in each vessel was 3 h. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol 97% at concentrations 1, 10 and 30 mg/L (both in range finder and main test)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 359.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 62.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 125 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 250 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Range-finder
A range-finder test with the concentrations of 10, 100 and 1000 mg/L was performed. The pH in the test vessels was 7.3, an adjustment was not necessary.
An inhibition between 49 and 58% was observed in the test vessels with 1000 mg/L test concentration. In the test vessels with the test concentration of 100 mg/L the observed inhibition was between 17 and 27%.
The temperature range of the range-finder test within the test duration was between 20.7 and 22.0°C.
Full test
The inhibiting effect of the test item on the total respiration of activated sludge was observed with six concentrations (62.5, 125, 250, 500, 1000, 2000 mg/L).
At the lowest test concentration 62.5 mg/L no significant different respiration rate than in the blank controls was observed. In the test vessels with 125.0 mg/L test concentration an inhibition of 21% (mean of five replicates), with 2000.0 mg/L test concentration an inhibition of 86% (mean of five replicates) was observed.
The pH in all test vessels at the beginning of the test vessels was between 7.3 and 7.4.
Blanks
The mean respiration rate of six blanks was 47.9 mg/(L*h) per one gram activated sludge. The coefficient of variation (CV) was 25% and therefore less than 30%. The pH in the blank vessels was 7.3. - Results with reference substance (positive control):
- In the full test the EC50 of 3,5-dichlorphenol was calculated as 5.8 mg/l and was therefore within the acceptable range of 2 to 25 mg/l. The pH in the reference vessels was 7.3.
- Reported statistics and error estimates:
- EC50, LOEC and NOEC were given by ToxRat Professional Version 3.2.1, © ToxRat Solutions GmbH
EC50 = 359.4 mg/L (95%-CL: 157.201 – 840.753 mg/L)
EC10 = 62.6 mg/L (95%-CL: 30.085 – 130.182 mg/L)
LOEC = 250.0 mg/L
NOEC = 125.0 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The results for the inhibition of the total respiration of activated sludge were:
EC50 = 359.4 mg/L (95%-CL: 157.201 – 840.753 mg/L)
EC10 = 62.6 mg/L (95%-CL: 30.085 – 130.182 mg/L)
LOEC = 250.0 mg/L
NOEC = 125.0 mg/L
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 359.4 mg/L
- EC10 or NOEC for microorganisms:
- 62.6 mg/L
Additional information
NOEC >= 125 mg/L
EC10 = 62.6 mg/L
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