Reaction products of phosphoryl trichloride and 2-methyloxirane

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of chemical substance in fish and shellfish", order of the prime minister, the minister of health and welfare, the minister of international trade and industry No 1, Japan, 1974

Principles of method if other than guideline:

This guideline corresponds to OECD Guideline 305C (Bioaccumulation: Test for the degree of bioconcentration in Fish), 1981

GLP compliance:

not specified

Specific details on test material used for the study:

Industrial product: TCPP
Composition: 3:1 mixture of tris(1-chloro-2-propyl) phosphate and tris(2-chloro-1-propyl) phosphate

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms: every two weeks, n=2, control fish analysis: before the initiation and the termination of exposure, n=2
- Sampling intervals/frequency for test medium samples: twice a week, n=1

Vehicle:

not specified

Details on preparation of test solutions, spiked fish food or sediment:

It is necessary to prepare a solution of the test substance 100 times highly concentrated than that in the aquarium. If the test substance is not soluble enough in water, suitable solubilisers were used. More detailled information is not available for the substance tested .

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Supplier: Sugishima fish farm, Japan
Weight: about 30g, Length: about 10 cm, Lipid content: 2-6 % (at the initiation of exposure)

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test temperature:

25+-2°C

Dissolved oxygen:

6-8 mg/L

Details on test conditions:

Test tank: 100L glass tank
Number of fish: 15-20 fish/Level
Renewal rate of test solutions: flow rate 200-800 mL/min

Nominal and measured concentrations:

Considering of the result of preliminary test for 48-hr LC50 and analytically detecting limit of the test substance, test concentrations were decided.
Nominal: 0.2 and 0.02 mg/l

Reference substance (positive control):

not specified

Lipid content:

3.5 %

Time point:

other: average

Type:

BCF

Value:

0.8 - 2.8

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Peak A

Remarks:

Conc.in environment / dose:0.2 mg/l

Type:

BCF

Value:

1.9 - 4.6

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: Peak A

Remarks:

Conc.in environment / dose:0.02 mg/l

Type:

BCF

Value:

1.3 - 2.1

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: peak B

Remarks:

Conc.in environment / dose:0.2 mg/l

Type:

BCF

Value:

14

Basis:

whole body w.w.

Calculation basis:

steady state

Remarks on result:

other: peak B

Remarks:

Conc.in environment / dose:0.02 mg/l

Details on results:

peak A: BCF at 0.02 mg/l was <1.9 - 4.6
peak B: BCF at 0.2 mg/l was <1.3 - 2.1
peak B: BCF at 0.02 mg/l was <14

Calculation of bioconcentration factor

BCF = concentration of test substance in fish/concentration of test substance in water

Validity criteria fulfilled:

yes

Remarks:

Since the test is poorly reported the validity criteria cannot be assessed sufficiently. Nevertheless the test is considered as valid based on the data given.

Conclusions:

With a BCF of 0.8 to 14 TCPP has no potential for bioaccumulation in aquatic organisms.

Executive summary:

In a bioaccumulation test, corresponding to OECD Guideline 305C a BCF of 0.8 - < 14 for TCPP was determined on Cyprinus carpio as test organism within 6 weeks (MITI 1992), indicating no potential for bioaccumulation in aquatic organisms.

Description of key information

With a BCF of 0.8 to 14 TCPP has no potential for bioaccumulation in aquatic organisms.

Key value for chemical safety assessment

BCF (aquatic species):

14 dimensionless

Additional information

In a bioaccumulation test, corresponding to OECD Guideline 305C a BCF of 0.8 - < 14 for TCPP was determined on Cyprinus carpio as test organism within 6 weeks, indicating no potential for bioaccumulation in aquatic organisms.

In this test analytical monitoring was carried out using GC/MS. The test substance was a mixture and several peaks are shown in the GC chromatogram. 2 main peaks were assigned to A and B in decreasing order of area on GC-MS.