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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 August 2011 to 18 March 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- Due to low aqueous solubility of the test substance, it was not possible to develop a suitable method of analysis with sufficient sensitivity to verify concentrations during the study. Therefore, results are reported in terms of the nominal loading rates.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
The test medium (100 mg/L) was prepared by weighing the test substance (100 mg) onto a glass cover slip, which was then added to a volumetric flask (1 L) and made up to volume with dilution medium. The contents of the flask were stirred overnight in the dark before the stirrer was turned off and a siphon then inserted at the mid-vessel point. The bulk medium was then left to stand overnight, after which the mid-vessel fraction was siphoned off and used in the test (nominal loading rate of 100 mg/L WAF). - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia Magna
- Strain: Straus
- Source: in-house culture originating from a reputable supplier
- Feeding during test: no feed was given during the exposure period
- Method of breeding: Cultures were fed daily with a suspension of the unicellular green algae, Pseudokirchneriella subcapitata, to provide nominally 0.1 to 0.2 mg carbon per daphnid, per day, except during the initial three days when a slightly lower ration was given. Culture conditions ensure that the stock animals reproduce by parthenogenesis. The day before the start of the study, all juvenile Daphnia present were removed from the laboratory cultures. The following morning, juveniles produced by the gravid (egg-bearing) adult Daphnia were removed from the culture vessels and held in a separate holding vessel; these animals, which were less than 24 hours old, were used in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- None
- Hardness:
- 252 mg/L as CaCO3
- Test temperature:
- 20.8 - 20.5 °C
- pH:
- 7.83 - 7.97
- Dissolved oxygen:
- 96 - 103 (% Air Saturation Value)
- Salinity:
- Not applicable to a freshwater study.
- Nominal and measured concentrations:
- Nominal concentration = 100 mg/L
Measured concentrations were not determined due to the very low water solubility of the test substance. - Details on test conditions:
- TEST SYSTEM
- Test vessel: dish
- Type: loosely covered
- Material, size, headspace, fill volume: glass containing 100ml of test medium
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 20ml/daphnia
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised, reverse osmosis water.
- Culture medium: Elendt M4 culture medium
- Test medium: Elendt M4 culture medium
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 hours light : 8 hours dark with periods of subdued lighting at the beginning and end of each light phase
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Daphnia were considered to be immobile if they were unable to swim within approximately 15 seconds following gentle agitation of the test vessel. The numbers of mobile, immobile and floating Daphnia were counted approximately 24 and 48 hours after the start of the study.
TEST CONCENTRATIONS
The range finding study employed nominal loading rates (WAFs) of 1, 10 and 100 mg/L. After 48 hours, all of the Daphnia were mobile at all nominal loading rates. Based on these results, the definitive (limit) test employed a single nominal loading rates of 100 mg/L.
- Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobilisation or effects on the Daphnia were noted.
Based on these findings, neither the 48-hour EL50 nor the no-observed-effect loading rate could be identified - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable
- Validity criteria fulfilled:
- yes
- Remarks:
- For the study to be valid the number of floating and/or immobile Daphnia in the control group must not be exceed 10% at the end of the test and the concentrations of dissolved oxygen must be > 3 mg/L at the end of the test.
- Conclusions:
- LZ1554 was not found to be acutely toxic to Daphnia magna when dispersed in water at a nominal loading rate of 100 mg/L.
- Executive summary:
The acute daphnia toxicity was determined in a GLP-compliant study in accordance with OECD no. 202 and EC test method C.2. LZ1554 was not found to be acutely toxic to Daphnia magna when dispersed in water at a nominal loading rate of 100 mg/L.
Reference
Description of key information
Invertebrates
LZ1554 was not found to be acutely toxic to Daphnia magna when dispersed in water at a nominal loading rate of 100 mg/L. Hence, the48 hour EL50 and NOELR were determined to be > 100 mg/L nominal.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Remarks:
- nominal concentrations
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.