Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-700-2 | CAS number: 1369492-55-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 February 2011 to 31 March 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant , guideline study. Acceptable without restriction.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Aerobic activated sludge from a wastewater treatment plant (ARA Ergolz II, Füllinsdorf, Switzerland) treating predominantly domestic wastewater was used.
- Preparation of inoculum for exposure: The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated. Based on the ratio of wet to dry weight calculated amounts of wet sludge were suspended in test water (see below) to obtain a concentration equivalent to 4 g (±10%) dry material per litre. During the holding period of three days prior to use, the sludge was aerated at room temperature. Prior to use, the sludge was first thoroughly mixed and then diluted with test water to a concentration of 1 g per litre (dry weight basis). Based on the determined dry weight of this diluted activated sludge, defined amounts were added to test water to obtain a final concentration of 30 mg dry material per litre.
- Pretreatment: None reported
- Concentration of sludge: 30 g dry material/L
- Duration of test (contact time):
- 28 d
- Initial conc.:
- >= 100 - <= 101 mg/L
- Based on:
- test mat.
- Initial conc.:
- 168 mg/L
- Based on:
- other: ThOD based on the absence of nitrification
- Initial conc.:
- 193 mg/L
- Based on:
- other: ThOD based on complete nitrification to NO3
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Purified water with dissolved analytical grade salts
- Test temperature: 22 °C
- pH: start: 7.3-7.4, end: 7.4 - 7.8
- pH adjusted: yes
- Aeration of dilution water: Test media was constantly stirred
- Suspended solids concentration: 30 mg dry material per litre.
- Continuous darkness: yes
- Other: SAPROMAT D12 (Voith GmbH, Heidenheim, Germany) used to incubate samples
TEST SYSTEM
- Culturing apparatus: 500ml reaction air tight flask containing 250 ml of test medium.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Test media was constantly stirred, consumed oxygen replenished daily by electrolytically generated oxygen from a copper sulphate solution.
- Measuring equipment: Oxygen consumption measured by absorbing CO2 in soda lime and detecting pressure drop by electrode type manometer. Oxygen consumption recorded manually each day.
- Details of trap for CO2 and volatile organics if used: CO2 trapped in soda lime
SAMPLING
- Sampling frequency: All test solutions sampled daily for 28 days
- Sampling method: CO2 absorbed on soda lime and pressure drop in airtight system measured with electrode type manometer. The consumed oxygen replaced by electrolytically generated oxygen from a copper sulphate solution.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes.
- Abiotic sterile control: No
- Toxicity control: Yes (test item plus reference item)
- Procedure control: Yes (reference item)
% biodegradation = [BOD (mg O2/mg chemical)/ThOD (mg O2/Mg chemical)] x 100%
BOD (Biochemical oxygen demand of the test or reference item) = (mg O2uptake/L by test/or reference item) - (mgO2 uptake/L by inoculum control)/mg test and/or reference item per litre - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- None reported
- Test performance:
- In the procedure controls, the reference item was degraded by an average of 88% by Exposure Day 14, thus confirming suitability of the activated sludge.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Based on a theoretical oxygen demand of 1.67 mg O2/mg test item without nitrification.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Remarks on result:
- other: Based on a theoretical oxygen demand of 1.92 mg O2/mg test item with nitrification.
- Details on results:
- Results are given as the average of the degradation in the two replicates.
The biochemical oxygen demand (BOD) of the test item in the test media was in the normal range found for the inoculum controls throughout the study period of 28 days. Consequently, was not biodegradable under the test conditions within 28 days.
In the toxicity control the course of biodegradation over the 28-day exposure period was similar to the two procedure controls, containing only the reference item. Within 14 days of exposure, biodegradation amounted to 43% and 40% based on the ThODNH4 and ThODNO3, respectively. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 101 mg/L because biodegradation in the toxicity control was >25% within 14 days. - Results with reference substance:
- In the procedure controls, the reference item was degraded by an average of 88% by Exposure Day 14, thus confirming suitability of the activated sludge. At the end of the test (Day 28), the reference item was degraded by an average of 96%.
- Validity criteria fulfilled:
- yes
- Remarks:
- In the procedure controls, the reference item was degraded by an average of 88% by Exposure Day 14.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test material was found not to be biodegradable under the conditions of the test. The results from the toxicity control show that the test item is not inhibitory to activated sludge.
- Executive summary:
The ready biodegradability of the test material was determined in a GLP compliant guideline study by observing the oxygen consumption over 28 days. A blank control, procedure control (sodium benzoate) and a toxicity control (sodium benzoate and test material) were also incubated for 28 days. Aerobic activated sludge was used as the inoculum. The validity criteria of the test were met. The test material was found not to be biodegradable under the conditions of the test within 28 days. The results from the toxicity control show that the test material is not inhibitory to activated sludge.
Reference
Table 2 Biodegradation in the Test Flask | |||||||||
Percentage Biodegradation1 | |||||||||
Test Item based on | Procedure control based on | Toxicity control based on | |||||||
ThODHN4 | ThODNO3 | ThOD | ThODHN4 | ThODNO3 | |||||
Time | Replicate No. | Replicate No. | Replicate No. | Replicate No. | |||||
Days | 1 | 2 | 1 | 2 | 1 | 2 | 1 | 2 | |
0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
1 | -1 | 0 | -1 | 0 | 13 | 13 | 7 | 7 | |
2 | -1 | -1 | -1 | -1 | 56 | 56 | 28 | 26 | |
3 | -- | -- | -- | -- | -- | -- | |||
4 | -1 | 0 | -1 | 0 | 73 | 73 | 37 | 34 | |
5 | -1 | 0 | -1 | 0 | 78 | 78 | 39 | 36 | |
6 | -1 | 0 | -1 | 0 | 81 | 81 | 40 | 37 | |
7 | -1 | 0 | -1 | 0 | 81 | 81 | 40 | 37 | |
8 | -1 | 0 | -1 | 0 | 82 | 82 | 41 | 38 | |
9 | -- | -- | -- | -- | -- | -- | -- | -- | |
10 | -- | -- | -- | -- | -- | -- | -- | -- | |
11 | -1 | 0 | -1 | 0 | 85 | 86 | 42 | 39 | |
12 | -1 | 0 | -1 | 0 | 86 | 87 | 43 | 40 | |
13 | -1 | -1 | -1 | -1 | 87 | 88 | 43 | 40 | |
14 | -1 | -1 | -1 | -1 | 87 | 89 | 43 | 40 | |
15 | -1 | -1 | -1 | -1 | 88 | 89 | 43 | 40 | |
16 | -- | -- | -- | -- | -- | -- | -- | -- | |
17 | -- | -- | -- | -- | -- | -- | -- | -- | |
18 | -1 | -1 | -1 | -1 | 90 | 91 | 44 | 41 | |
19 | -2 | -1 | -2 | -1 | 91 | 92 | 44 | 41 | |
20 | -1 | -1 | -1 | -1 | 91 | 93 | 44 | 41 | |
21 | -1 | -1 | -1 | -1 | 92 | 93 | 45 | 42 | |
22 | -1 | -1 | -1 | -1 | 93 | 94 | 45 | 42 | |
23 | -- | -- | -- | -- | -- | -- | -- | -- | |
24 | -- | -- | -- | -- | -- | -- | -- | -- | |
25 | -1 | -1 | -1 | -1 | 94 | 90 | 46 | 42 | |
26 | -1 | -1 | -1 | -1 | 94 | 96 | 46 | 43 | |
27 | -1 | -1 | -1 | -1 | 95 | 96 | 46 | 43 | |
28 | -1 | -1 | -1 | -1 | 96 | 97 | 47 | 44 | |
Mean (28 days) | (-1)* | (-1)* | 96 | Not applicable | |||||
1 Corrected for mean oxygen uptake of the inoculum controls | |||||||||
-- Not determined | |||||||||
* Negative value due to higher oxygen consumption in the inoculum controls than in the test flasks with test item |
Description of key information
The result of the study indicates that the substance is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Only a single experimental study for biodegradation in water are available for the test substance. The result of the study indicates that the substance is not readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.