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Diss Factsheets
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EC number: 617-769-9 | CAS number: 858956-08-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 Jul - 02 Aug 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations at 0 h and 48 h
- Sampling method: collected at mid-depth of vessel
- Sample storage conditions before analysis: processed immediately - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the highest test concentration was prepared by mixing the required amount of test substance with dilution water. The test concentrations were prepared separately by dilution of the stock solution.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Age at study initiation: < 24 h
- Method of breeding: same conditions as in test
- Source: bred at testing facility
- Feeding during test : no
ACCLIMATION
- Acclimation period: 21 d
- Acclimation conditions (same as test or not): breeding conditions same as in test
- Food type: mixture of yeast, cereal grass media and trout chow
- Frequency: daily
- Health during acclimation (any mortality observed): no mortality observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 136 mg/L as CaCO3
- Test temperature:
- 19.0 - 20.3 °C
- pH:
- 7.7 - 8.4
- Dissolved oxygen:
- 8.4 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal test substance concentrations: 3.8, 7.5, 15, 30, 60 and 120 mg a.s./L
Mean meas. test substance concentrations: 3.7, 7.4, 15, 30, 59 and 120 mg a.s./L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Fill volume: 220 mL
- Headspace: 30 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water located on the Wildlife International, Ltd. site, passed through sand filter and aerated. Prior to use filtered to 0.45 μm and passed through an ultraviolet sterilizer.
- Total organic carbon: <1 mg C/L
- Alkalinity:184 mg/L as CaCO3
- Conductivity: 310 μmhos/cm
- Intervals of water quality measurement: at 0, 24 and 48 h
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day-nigh cycle
- Light intensity: Fluorescent light 198 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mobility after 5, 24 and 48 h - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 43 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% Fiducial Interval: 30 and 69 mg a.s./L
- Details on results:
- - Behavioural abnormalities:
lethargy at test concentrations of 15 mg/L after 24 h
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses: no - Reported statistics and error estimates:
- Probit analysis, the moving average method, and binomial probability with nonlinear interpolation using software of Stephan, C.E., U.S. EPA. 1978.
- Validity criteria fulfilled:
- yes
- Remarks:
- Please see Table 2 at "Any other information on results and tables" for more details
Reference
Table 1: Immobilisation and sublethal effects after 48 hours
Mean, Measured Concentration [mg a.s./L] |
Cumulative number of immobilised/ total number of daphnids after 48 h |
Observations |
3.7 |
0/20 |
- |
7.4 |
0/20 |
- |
15 |
6/20 |
- |
30 |
11/20 |
- |
59 |
8/20 |
3 lethargic specimen |
120 |
16/20 |
4 lethargic specimen |
control | 0/20 | - |
Table 2: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
> 8 mg/L |
yes |
Description of key information
EC50 (48 h) = 43 mg/L (mean measured, OECD 202, Daphnia magna, immobility)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 43 mg/L
Additional information
One experimental study testing the acute toxicity of the test substance towards aquatic invertebrates is available.
This key freshwater
study was performed according
to GLP and OECD 202.
Daphnia
magna was exposed to concentrations ranging from 3.7 to 120 mg a.s./L
(mean measured). Test
substance concentrations were measured directly by
high performance liquid chromatography (HPLC) with UV detection. Mean
measured concentrations were between 99.2 to 101% of nominal
concentrations.
The
48-hour EC50 value, based on mobility,
was 43 mg a.s./L (mean measured, 95%-CI:
30 to 69 mg a.s./L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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