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Diss Factsheets
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EC number: 500-288-2 | CAS number: 103213-20-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 18 Jan - 20 Jan 2005
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP - Guideline study, tested with the source substance Fatty acids, C18-unsatd., dimers, 2-ethylhexyl esters (CAS 68334-05-4). According to the ECHA guidance document “Practical guide 6: How to report read-across and categories (ECHA, 2012)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.”
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Fatty acids, C18-unsatd., dimers, 2-ethylhexyl esters
- EC Number:
- 500-204-4
- EC Name:
- Fatty acids, C18-unsatd., dimers, 2-ethylhexyl esters
- Cas Number:
- 68334-05-4
- Molecular formula:
- C44H80O4
- Details on test material:
- - Name of test material: Fatty acids, C18-unsatd., dimers, 2-ethylhexyl esters
- Physical state: liquid
- Analytical purity: no data
- Storage condition of test material: in the dark, at ambient temperature, in the container in which it was received until required for testing.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples for analysis were taken from the centre of the test solutions at start of the test period (0 h).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accommodated Fractions (WAF) with a loading rate of 100 mg/L were prepared based on ASTM D6081-98 for this test. The test concentration was prepared by the addition of a known amount of the test substance to dilution water. This solution was stirred for 24 hours in the dark using a PTFE coated magnetic stirring bar and then allowed to settle for > 1 hour. The resultant solution was clear and colourless with an oily surface film visible. A portion of the sub-surface liquid was siphoned off for use in the study. The control consisted of dilution water only.
Dissolved organic carbon analysis of the nominal 100 mg/L WAF gave essentially the same values as the dilution water control, which had been similarly treated.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: from own continuous laboratory cultures. The culture was originally obtained from IRCHA, France and the Daphnia were subsequently characterised by Sheffield University, UK as Clone 5.
- Age at study initiation: Daphnia < 24 hours old (first instar), obtained from a single culture vessel, were used for testing. The parent animals were 29 ± 1 days old.
- Method of breeding: Daphnia reproduction by diploid parthenogenesis
- Feeding during test: none
ACCLIMATION
- Acclimation conditions: The stock cultures of Daphnia were maintained in a reconstituted water medium (M4 medium), identical to the test dilution water, at a temperature of 20 ± 1 °C. The cultures were maintained in 2 L glass vessels. A photoperiod of 16 hours light: 8 hours dark, with 20 minute dusk and dawn transition periods was provided.
- Type and amount of food: The Daphnia cultures were fed a defined diet of algae Chlorella vulgaris, strain CCAP 211/12 and a commercially available fish food.
- Health during acclimation: No evidence of disease was observed.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 216 mg /L as CaCO3
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.9 - 8.2
- Dissolved oxygen:
- 8.2 to 9.2 mg/L (> 60% oxygen saturation)
- Nominal and measured concentrations:
- Nominal: 100 mg/L loading rate WAF
- Details on test conditions:
- TEST SYSTEM
- Test vessel: narrow necked borosilicate glass bottles of 60 mL nominal capacity. The bottles were covered with silicon lids and clamp sealed.
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water, M4 Medium according to Elendt and Bias
- Total organic carbon: 0.75 mg/L
- Conductivity: 586 µS/cm
- Culture medium different from test medium: No
- Intervals of water quality measurement: After 0 and 48 hours
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light : 8 hours dark, with 20 minute dusk and dawn transition periods
EFFECT PARAMETERS MEASURED: Immobility were recorded after 24 and 48 hours test duration.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Limit test as WAF were prepared. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: WAF
- Basis for effect:
- mobility
- Details on results:
- No immobilised daphnia were observed within this study - neither in the test concentrations nor in the controls. No symptoms of toxicity were observed in this study.
Any other information on results incl. tables
The test report mentions a NOEC of 100 mg/L, but due to the fact that WAFs were prepared a NOELR has to be reported instead.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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